<p>The Overexpression of Kinesin Superfamily Protein 2A (KIF2A) was Associated with the Proliferation and Prognosis of Esophageal Squamous Cell Carcinoma</p>

Abstract

Aim: Kinesin family member 2A (KIF2A) is a member of the kinesin-13 superfamily protein. KIF2A played a role in the development of many tumors. However, the role of KIF2A in esophageal squamous cell carcinoma (ESCC) remains unclear. In this study, we aimed to investigate the role of KIF2A in ESCC. Methods: We used bioinformatics analysis to study the expression levels and prognosis of KIF2A in ESCC and normal tissues. We also used our own samples to verify the results by immunohistochemistry. Then, the biological functions of KIF2A in ESCC was studied by cell experiments and animal experiments. Results: Both the TCGA database and our samples showed that KIF2A was relatively highly expressed in ESCC tissues and was significantly associated with disease-free survival (P =0.037) in TCGA database. Colony formation assay, CCK8 and Western blotting results showed that knockdown of KIF2A can significantly reduce colony forming ability and proliferation ability. The results of animal experiments showed that knocking down KIF2A can significantly reduce the tumor volume of mice. Conclusion: KIF2A might be used as a prognostic factor for ESCC, and knockdown of KIF2A could inhibit ESCC proliferation in vitro and in vivo, respectively. KIF2A could serve as a potential prognostic biomarker and therapeutic target for future ESCC.

Kinesin family member 2A links with advanced tumor stage, reduced chemosensitivity and worse prognosis in gastric cancer

“…Regarding the correlation of KIF2A with prognosis, previous studies have been performed to investigate its association with prognosis in various tumors. 13 , 14 , 17 , 24 For example, upregulated KIF2A independently associates with worse OS in facilitate lung adenocarcinoma patients 13 ; EOC patients with overexpression of KIF2A has a shorter OS. 24 Our study found that tumor KIF2A high was associated with poor accumulating OS in GC patients, which was similar to the findings of a previous study.…”

“… 16 Clinically, KIF2A could serve as a potential prognostic biomarker for facilitate prognostication of lung adenocarcinoma and esophageal squamous cell carcinoma patients. 13 , 17 Based on the above‐mentioned information, we hypothesized that KIF2A might be a potential biomarker for GC. However, few previous studies had been performed.…”

Kinesin family member 2A acts as a potential prognostic marker and treatment target via interaction with PI3K/AKT and RhoA/ROCK pathways in acute myeloid leukemia

“…However, KIF2A has been found to be correlated with clinical features or prognosis in other types of cancer. For example, a previous study based on The Cancer Genome Atlas database demonstrated that KIF2A is upregulated in esophageal squamous cell carcinoma tissue and its expression is correlated with worse disease-free survival in patients ( 19 ). Another study observed increased expression of KIF2A in patients with gastric cancer, and KIF2A expression is associated with worse histological type, higher TNM stage, lymph node metastasis and decreased 5-year survival rate; in addition, KIF2A is also an independent predicting factor for worse prognosis in patients with gastric cancer ( 20 ).…”

Aberrant kinesin family member 2A signifies tumor size and invasion, and may help predict prognosis of patients with papillary thyroid carcinoma

“…The relative expression of KIF2A was calculated based on the 2 −ΔΔCq method and β-actin was used as the internal reference ( 20 ). The primers for KIF2A and β-actin were designed as follows: KIF2A forward, 5′-GCCTTTGATGACTCAGCTCC-3′ and reverse, 5′-TTCCTGAAAAGTCACCACCC-3′; β-actin forward, 5′-TGACGTGGACATCCGCAAAG-3′ and reverse, 5′-CTGGAAGGTGGACAGCGAGG-3′ ( 21 ).…”