<p>The Regulatory Role of circRNA_101308 in Cervical Cancer and the Prediction of Its Mechanism</p>

Abstract

Purpose: Accumulating evidence indicates that circular RNAs (circRNAs) are closely involved in canceration and cancer progression. However, the role of circRNAs in cervical cancer (CC) is largely unknown. Here, we characterized the role of circRNA_101308 in CC. Materials and Methods: The expression of circRNA_101308 in CC tissues was measured by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Then, circRNA_101308 was overexpressed in CC cells to detect its function by proliferation and apoptosis assays, Transwell assays and animal experiments. The potential mechanism of circRNA_101308 in CC was explored by RNA pull-down, Gene Ontology (GO) and pathway analyses. Results: CircRNA_101308 was significantly downregulated in CC tissues. The level of circRNA_101308 was much lower in CC patients with lymph node metastasis or deep myometrial invasion compared to those patients without lymph node metastasis and superficial myometrial invasion. CircRNA_101308 overexpression inhibited CC cell proliferation, invasion and migration. MiR-26a-5p, miR-196a-5p, miR-196b-5p, miR-335-3p, and miR-1307-3p were found to be sponged by circRNA_101308 in CC cells. Further, GO and pathway analyses predicted the potential functional processes and pathways of circRNA_101308 in CC. Conclusion: CircRNA_101308 is downregulated and acts as a tumor suppressor in CC. CircRNA_101308 can participate in many different processes by sponging different miRNAs in CC cells. This exploration of circRNA_101308 provides new directions for research on cancer development and the clinical treatment of CC.

The diagnostic and prognostic values of microRNA-196a in cancer

“…miR-196a-5p and miR-196a-3p are two , and we also previously reported that it was associated with occurrence of cancers [10]. As a regulator, it could be also regulated by non-coding RNAs, such as lncRNA FEZF-AS1 [11], circRNA 101308 [12], H19 [13], lncRNA SNHG1 [14], which were involved in tumorgenesis and tumor progression.…”

“…Meanwhile, polymorphism in miR-196a-2 was reported to confer occurrence risk or progression of cancers, such as it was associated with HCC recurrence after liver transplantation [ 9 ], and we also previously reported that it was associated with occurrence of cancers [ 10 ]. As a regulator, it could be also regulated by non-coding RNAs, such as lncRNA FEZF-AS1 [ 11 ], circRNA 101308 [ 12 ], H19 [ 13 ], lncRNA SNHG1 [ 14 ], which were involved in tumorigenesis and tumor progression.…”

Circular RNAs: Novel biomarkers for cervical, ovarian and endometrial cancer (Review)

“…Another circRNA, circ_0005576, has been associated with a higher pathological stage and lymph node metastasis (50). However, alternative studies have indicated that reduced circRNA expression also promotes metastasis; Jiao et al (51) revealed that low expression levels of circRNA_101308 were associated with deep myometrial invasion and lymph node metastasis. Therefore, selecting the appropriate circRNA biomarker for a specific pathology requires more thorough consideration.…”

“…CircRNA_101308 expression was also revealed to be decreased in cervical cancer tissues and cell lines, compared with that in normal tissues. CircRNA_101308 can bind various different miRNAs and regulate their downstream genes to suppress the proliferation, invasiveness and metastasis of cervical cancer cells, both in vitro and in vivo (51). Furthermore, circ-ITCH has been revealed to be associated with the regulation of tumor growth, and the proliferation and apoptosis of cancer cells in bladder (59) and breast cancer (60).…”

Neutralizing shapeshifting pericytes enhances glioblastoma therapeutic efficacy

“…The studies in this paper interrogate the utility of MVC in preclinical GBM models. MVC and other CCL5-CCR5 pathway inhibitors are also undergoing clinical evaluation for multiple tumor types 6 . The CCL5-CCR5 signaling axis has been studied extensively and contributes to tumor progression both by direct effects on proliferation and by indirect induction of angiogenesis and recruitment of immune cells to the GBM microenvironment 7,8 .…”