&lt;title&gt;A new method of quantitative determination of apoptotic parameters in cellular suspensions&lt;/title&gt;

Bilyi, Olexander I.; Bilyy, Rostyslav; Getman, Vasyl B.

doi:10.1117/12.560052

Cited by 3 publications

(2 citation statements)

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“…Those cells were chosen as a testing model because their integrity can be easily evaluated by conventional methods (microscopy, agglutination observation), they were proven to be a good model for study the interaction with nanoparticles of different nature [9], besied the tests can be scaled up for automated analysis as described previously [10,11]. Human leukemia Jurkat T-cells were cultured in RPMI 1640 culture medium supplemented with 10% of heat inactivated fetal calf serum (Sigma) and gentamycin (50 mg/ml, Sigma).…”

Section: Methodsmentioning

confidence: 99%

The interaction of the carbon nanoparticles with human cell plasma membrane

et al. 2013

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The study of carbon nanostructures is a highly topical branch of bionanotechnology because of their potential application in biomedicine. Carbon nanotubes (CNTs) are known for their ability to kill tumor cells causing hyperthermia shock and can be used in photothermal therapy respectively. Also chemically modified CNTs can be used for drug delivery. The needle-like shape of CNTs allows them to penetrate into the cell plasma membrane without killing the cell. C 60 fullerenes are regarded as valuable nanocarriers for different hydrophobic molecules as well as potential antiviral agents or photosensitizers.In our previous studies we have demonstrated that all types of carbon nanoparticles cause externalization of phosphatidylserine (PS) from the inner to the outer layer of the cell membrane in the small local patches (points of contact), leaving the other parts of plasma membrane PS-negative. In the current work there were studied the interactions of pristine C 60 fullerenes and different types of CNTs with human blood cells (erythrocytes and Jurkat T-cells). We have shown, that carbon nanoparticles do not have any hemolytic effects, if judged by the dynamics of acidic hemolysis, although they are capable of permeabilizating the cells and facilitating the internalization of propidium iodide into the nuclei.

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Section: Methodsmentioning

confidence: 99%

The interaction of the carbon nanoparticles with human cell plasma membrane

et al. 2013

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“…F U t is measured value, ( , , ) K U t r is the kernel function, which depends on the photoreceiver registers the array of impulses with amplitudes U and durations t, measuring, r is a size parameter, ( ) n r is the size distribution function, n(r)dr is the number of particles in the size range[ , ] The algorithm of the solution of Fredgholm integral equation(6) are presented in[24,25].3. PROCEDURE OF MEASURINGDetermination of quantity of bacterial cells in the process of their cultivation is achieved by conducting of by turn researches of time-domain measurements of light scattering parameters of liquid growth media which the bacterial cells are cultivated in, with cellular objects, and without them.…”

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Rapid detection of bacterial cells by light scattering method

et al. 2008

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Method of rapid detection of bacterial cells by light scattering is described. Determination of quantitative changes of bacteria is the given method based on the changes of their size distributing in the process of cultivation. Liquid medial are diluted and analyzed by the proposed technology to determine presence of bacteria. A method includes sounding of flow suspended bacterial cells by monochromatic coherent light, registration of signals of co-operation of sounding radiation with the explored microbiological objects by detects amplitudes and durations of scattered light impulses. Distribution of particles by sizes is determined from the measured functional dependence of number of registered particles from amplitude and duration of the proper electric impulses on the output photoreceiver. Detection is done for a range of particle size from 0.1 to 10 mkm, and thus particle's size distribution is determined. The results of studying of rapid detection of Escherichia coli by light scattering are described.

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Light scattering application for bacterial cell monitoring during cultivation process

Kotsyumbas

Kushnir

Bilyy

et al. 2007

Novel Optical Instrumentation for Biomedical Applications III

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Monitoring of bacterial cell numbers is of great importance not only in microbiological industry but also for control of liquids contamination in the food and pharmaceutical industries. Here we describe a novel low-cost and highly efficient technology for bacterial cell monitoring during cultivation process. The technology incorporates previously developed monitoring device and algorithm of its action. The devise analyses light scattered by suspended bacterial cells. Current stage utilizes monochromatic coherent light and detects amplitudes and durations of scattered light impulses, it does not require any labeling of bacterial cell. The system is calibrated using highly purificated bacteria-free water as standard. Liquid medial are diluted and analyzed by the proposed technology to determine presence of bacteria. Detection is done for a range of particle size from 0.1 to 10 um, and thus particles size distribution is determined. We analyzed a set of different bacterial suspensions and also their changes in quantity and size distribution during cultivation. Based on the obtained results we conclude that proposed technology can be very effective for bacteria monitoring during cultivation process, providing benefits of low simplicity and low cost of analysis with simultaneous high detection precision.

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<title>A new method of quantitative determination of apoptotic parameters in cellular suspensions</title>

Cited by 3 publications

References 1 publication

The interaction of the carbon nanoparticles with human cell plasma membrane

The interaction of the carbon nanoparticles with human cell plasma membrane

Rapid detection of bacterial cells by light scattering method

Light scattering application for bacterial cell monitoring during cultivation process

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