Laser skin perforation is an e®ective and promising technique for use in blood collection. In this study, the relation between the perforation pro¯le of skin and laser irradiation at various energies is discussed. Increasing laser energy does not uniformly expand the size and depth of a hole because the shallow depth of¯eld (DOF) of the focused light primarily concentrates energy on the skin surface. In practice, the hole gradually transforms from a semielliptical shape to an upsidedown avocado shape as the laser energy increases. This phenomenon can increase the amount of bleeding and reduce pain. The¯ndings support the feasibility of developing an accurate laser skin perforation method.