&lt;title&gt;Interaction of photosensitizers with membranes of liposomes and of erythrocytes&lt;/title&gt;

Klebanov, G. I.; Stranadko, Eugeny Ph.; Teselkin, Yu. O.; Babenkova, I. V.; Chichuk, T. V.

doi:10.1117/12.260758

Cited by 16 publications

(19 citation statements)

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“…--suspension of liposomes obtained by homogenization of egg yolk in phosphate buffer (1:5) [4]. Preparations in concentrations compatible with therapeutic doses were added to model systems, and the maximum amplitude of slow flash, light sum, and latent period between fast and slow CL flashes were recorded.…”

Section: Methodsmentioning

confidence: 99%

Effects of different antibiotics on free radical oxidationIn Vitro andIn Vivo

et al. 1998

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Effects of naturally occurring and semisynthetic penicillins, cephalosporines, aminoglycosides, tetracyclines, and lincomycin on free-radical oxidation were studied in 4 model systems and in intact Wistar rats. Liver and kidney tissue and blood plasma were examined by chemiluminescence. Antibiotics of all groups in therapeutic concentrations exerted antioxidant effect in vitro and in vivo. Key Words: antibiotics; free-radical oxidation; chemiluminescenceAntibiotics play the leading role in chemotherapy of bacterial infections [6]. Impaired regulation of freeradical oxidation (FRO) is a nonspecific component in the pathogenesis of many diseases, including diseases of bacterial etiology [3]. Today we know that many drugs possessing pro-and antioxidant properties can influence FRO [2]. From this viewpoint, new approaches to antibiotic therapy can be developed, aimed at altering FRO, i. e., the production of active oxygen forms and lipid peroxidation (LPO). A great variety of antibiotics (more than 6000) and their great significance in the management of inflammatory processes prompt the search for simple test systems for predicting effects on FRO [2,3,8].We 'studied of the effects of widely used antibioties on FRO in vitro and in vivo using the chemiluminescent (CL) registration [2,3,7]. Our objectives were as follows:1. Study of antibiotic effects in model systems generating active oxygen forms and in a liposome suspension in which LPO reactions occur.2. Study of antibiotic effect on LPO in vivo in blood plasma and hepatic and renal tissue of intact Wistar rats after an 8-day course of intramuscular injections in therapeutic concentrations.

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Section: Methodsmentioning

confidence: 99%

Effects of different antibiotics on free radical oxidationIn Vitro andIn Vivo

et al. 1998

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“…Free-radical oxidation was measured in a model system consisting of multilayer liposomes prepared from egg-yolk lipoproteins as described [3]. The intensity of FRO was assessed by the kinetics of Fe2+-induced chemiluminescence (CL), and the concentration of thiobarbituric acid reactive substances (TBARS) was determined as described elsewhere [5].…”

Section: Methodsmentioning

confidence: 99%

“…The concentration of TBARS and antioxidant activities of the drugs in a model system of multilayer liposomes from egg-yolk lipoproteins were determined by standard methods [3,5].…”

Section: Methodsmentioning

confidence: 99%

“…However, these drugs display a broad spectrum of pharmacological activities. For example, some antihistamine preparations inhibit free-radical reactions [3,4]. Elucidation of the mechanisms responsible for the antioxidant activity of antihistamine drugs can be helpful in expanding the range of indications for these drugs, since the inhibitors of free-radical oxidation (FRO) are known to increase cell resistance to damaging factors by stabilizing plasma membranes.…”

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The antioxidant properties of histamine receptor blockers: a comparative study in a model system

Сергеев

Chukaev

1996

Bull Exp Biol Med

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Antioxidant activities of seven histamine receptor blockers are compared using a model system of multilayer liposomes, chemiluminescence analysis, and 2-thiobarbituric acidreactive substance assay. According to a decrease in the antioxidant activity, the drugs can be arranged as follows: diprazine, suprastin, tavegil, diazoline, dimedrol, phencarol, and ranitidine. The possible mechanisms of the antioxidant activity and the significance of the antioxidant properties of these drugs for experimental research and clinical practice are discussed.

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“…After 3 weeks the rats were decapitated, and the blood was collected. Plasma AOA activity was measured in a model system of yolk lipoprotein oxidation [5] by the concentrations of TBAactive agents using Biokont TBK kits. The data were statistically analyzed using Student's t test.…”

mentioning

confidence: 99%

Antioxidant effects of solusulfonum and α-tocopherol

et al. 2000

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The effects of t~-tocopherol and solusulfonum on LPO and plasma antioxidant activity were compared in Wistar rats. The data attest to a pronounced antioxidant effect and synergy of these preparations.Key Words: antioxidant activity; LPO, solusulfonum; t~-tocopherol Stability of total antioxidant activity (AOA) in tissues, its peculiarity in each organ, and the intensity of free radical oxidation (FRO) are the basic indices of homeostasis [3]. Changes of these indices are essential for the pathogenesis of some diseases [4]. Since application of antioxidants as nonspecific therapeutic agents modulates the interaction between the antioxidant mechanisms and FRO processes, the specificity of the action of natural and synthetic antioxidants needs to be studied.Numerous works of the last decades established the key role of biological antioxidants in protective and adaptive reactions of the organism, which can be explained by their capacity to regulate the state of cell membranes [1][2][3][4]. A particular role in this regulation is played by t~-tocopherol, which participates in maintaining FRO at a physiological level. According to the antioxidant theory, ~-tocopherol acts as a biological antioxidant by inactivating free radicals thereby preventing the development of nonenzymatic free radical peroxidation of unsaturated lipids in tissues with molecular oxygen [7].Some antibacterial preparations, in particular sulfones, used as antilepric drugs possess antioxidant properties and can affect LPO [6]. They are also used in the treatment of other infectious diseases and are the drugs of choice in the treatment of herpetiform (Duhring's) dermatitis. However. it is not clear whether the therapeutic effect of sulfones depends on their antioxidant properties. Our aim was to compare the [nstitute of Lepra, Astrakhan antioxidant effects of solusulfonum and t~-tocopherol under the normal physiological conditions, MATERIALS AND METHODSThe study was carried out on male Wistar rats weighing 200 g. The intensity of AOA and the content of malonic dialdehyde (MDA) were determined in rats treated with c~-tocopherol (10% oil solution, 20 mg/kg) and solusulfonum (25 and 125 mg/kg). The rats were divided into five groups (7 rats per group). Group 1 was control. Group 2 rats received (~-tocopherol in a dose of 20 mg/kg, groups 3 and 4 rats received solusulfonum in doses of 25 and 125 mg/kg, respectively, and group 5 rats received solusulfonum (125 mg/kg) and ~-tocopherol (20 mg/kg). Water solution of solusulfonum was injected intramuscularly 2 times a week. while o~-tocopherol was given perorally according to the same scheme. After 3 weeks the rats were decapitated, and the blood was collected. Plasma AOA activity was measured in a model system of yolk lipoprotein oxidation [5] by the concentrations of TBAactive agents using Biokont TBK kits. The data were statistically analyzed using Student's t test.

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<title>Interaction of photosensitizers with membranes of liposomes and of erythrocytes</title>

Cited by 16 publications

References 0 publications

Effects of different antibiotics on free radical oxidationIn Vitro andIn Vivo

Effects of different antibiotics on free radical oxidationIn Vitro andIn Vivo

The antioxidant properties of histamine receptor blockers: a comparative study in a model system

Antioxidant effects of solusulfonum and α-tocopherol

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