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Using photochemiluminescence, the interaction between carnosine and superoxide anion was measured directly. Carnosine at physiological concentrations decreased the amplitude of luminol chemiluminescence like superoxide dismutase (SOD) did, and prolonged the lag‐period of the chemiluminescence similar to the effect of ascorbic acid. From the interaction of nitro blue tetrazolium with superoxide anion generated by the xanthine oxidase system, the constant for interaction of carnosine with O2* was calculated to be 105 M‐1. sec‐1. The possible biological significance of the quenching of superoxide anion by carnosine is discussed.

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Dihydroquercetin is a natural flavonoid with antioxidant properties that are determined by its ability to scavenge free radicals and chelate transition metal ions. Chemiluminescent studies showed that iron (II) ions in complex with dihydroquercetin are inactive and cannot catalyze decomposition of hydrogen peroxide (Fenton reaction).

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