&lt;title&gt;Near-infrared fluorescent probes&lt;/title&gt;

Casay, Guillermo A.; Czuppon, Tibor; Lipowski, Jacek; Patonay, Gábor

doi:10.1117/12.144722

Cited by 3 publications

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“…Recently, the fluorescence technology has been widely used in the phototherapy, diagnosis, visual detection, and other fields. − The advantages of fluorescent probes such as noninvasive, real-time sampling capability, and sensitive and high spatiotemporal resolution make it much more convenient for the specific assay of target enzymes. − Among various fluorophores, the near-infrared (NIR) fluorescent probes exhibited some prominent advantages owing to its excellent tissue penetration and low background fluorescence, which allowed the visual detection of some vital metabolic enzymes for in vivo imaging. − …”

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Endoplasmic Reticulum-Targeting Near-Infrared Fluorescent Probe for CYP2J2 Activity and Its Imaging Application in Endoplasmic Reticulum Stress and Tumor

et al. 2022

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CYP2J2 as an endoplasmic reticulum (ER)-expressed vital cytochrome P450 isoform participates in the metabolism of endogenous polyunsaturated fatty acids. Its abnormal expression and function are closely related to the progress of cancer and cardiovascular diseases. Herein, an ERtargeting near-infrared (NIR) fluorescent probe ER-BnXPI was developed for monitoring CYP2J2 activity, which possessed a high selectivity and sensitivity toward CYP2J2 among various CYP450 isoforms and exhibited excellent subcellular localization for ER. Then, the CYP2J2 variation behavior under the ER stress model was imaged by ER-BnXPI in living cells and successfully used for the in vivo imaging in different tumors that well distinguished tumor tissues from para-cancerous tissues. All these findings fully demonstrated that ER-BnXPI could be used as a promising tool for exploring the physiological function of CYP2J2 and provided some novel approach for the diagnosis and therapy of CYP2J2-related vascular inflammation and cancer.

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Endoplasmic Reticulum-Targeting Near-Infrared Fluorescent Probe for CYP2J2 Activity and Its Imaging Application in Endoplasmic Reticulum Stress and Tumor

et al. 2022

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“…The addition of sulfonate groups to the dye structures greatly improves their solubility, and the addition of reactive groups such as isothiocyanate functionalities makes them suitable for use as covalent labels . Reported uses of NIR dyes for analytical applications include enzymatic assays, hydrophobicity, metal ion and pH probes, immunoassay applications, and as labels for DNA sequencing studies. − …”

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Ultrasensitive Detection of Closely Related Angiotensin I Peptides Using Capillary Electrophoresis with Near-Infrared Laser-Induced Fluorescence Detection

Baars

Patonay

1998

Anal. Chem.

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A novel near-infrared (NIR) fluorescent dye (NN382, LICOR, Inc.) was evaluated as an ultrasensitive peptide-labeling reagent for use with capillary electrophoresis (CE). Six angiotensin I (Ang-I) variants were selected as model peptides for the derivatization and separation studies. The closely related decapeptides were labeled with the NIR dye, separated using CE, and detected by NIR laser-induced fluorescence. Derivatization of the peptides was achieved under aqueous conditions using 2.5-500 pmol of Ang-I in a 50-microL sample (5 x 10(-8)-1 x 10(-5)M), and between 1.3 and 254 amol of the labeled peptides were injected on column. The fluorescence response was linear over a 200-fold range (correlation r > or = 0.9986). The limit of detection (SNR = 3, signal/RMS noise) ranged from 100 to 300 zmol, for the six Ang-I variants. Four of six peptides were resolved from each other and excess dye using capillary zone electrophoresis with a simple 50 mM phosphate run buffer, pH 7.2. Two pairs of coeluting peptides were successfully resolved using micellar electrokinetic chromatography with a nonionic surfactant, Triton X-100. The NIR amine-labeling reagent NN382 is a viable alternative to using visible fluorophores for CE methods requiring high sensitivity.

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Near-Infrared Fluorescence Probes

Casay

Shealy

Patonay

Topics in Fluorescence Spectroscopy

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<title>Near-infrared fluorescent probes</title>

Cited by 3 publications

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Endoplasmic Reticulum-Targeting Near-Infrared Fluorescent Probe for CYP2J2 Activity and Its Imaging Application in Endoplasmic Reticulum Stress and Tumor

Endoplasmic Reticulum-Targeting Near-Infrared Fluorescent Probe for CYP2J2 Activity and Its Imaging Application in Endoplasmic Reticulum Stress and Tumor

Ultrasensitive Detection of Closely Related Angiotensin I Peptides Using Capillary Electrophoresis with Near-Infrared Laser-Induced Fluorescence Detection

Near-Infrared Fluorescence Probes

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