&lt;title&gt;Numerous applications of fiber optic evanescent wave Fourier transform infrared (FEW-FTIR) spectroscopy for subsurface structural analysis&lt;/title&gt;

Afanasyeva, Natalia I.; Welser, L. A.; Bruch, R.; Kano, Angelique; Makhine, Volodymyr

doi:10.1117/12.365688

Cited by 6 publications

(5 citation statements)

References 17 publications

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“…The bands located at 1086 and 1234 cm À1 are symmetric and asymmetric stretching associated with the phosphate groups associated with the DNA backbone (7,8), which is based on a repeated pattern of a sugar group and a phosphate group. The peptide amide I and II bands were identified at 1683 and 1540 cm À1 , respectively (8,(22)(23)(24). Aliphatics of the C-H group, which is comprised of biomolecules such as proteins, lipids and carbohydrates (10,23), were located at 1455, 2846 and 2938 cm À1 .…”

Section: Resultsmentioning

confidence: 99%

“…Then, to investigate the temporal evolution of these alterations in the chemical groups during the 8 weeks following infection, the ratio between the optical bands in each spectrum was obtained in relation to the most stable band, that of amide II (1545 cm −1 ), which is associated with the N–H group bending present in the amide protein group . The spectral changes were compared with the histological observations.…”

Section: Resultsmentioning

confidence: 99%

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Morphological and Structural Changes in Lung Tissue Infected by Paracoccidioides brasiliensis: FTIR Photoacoustic Spectroscopy and Histological Analysis

Morato

Morais

Sato

et al. 2013

Photochem & Photobiology

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This study evaluated physical, chemical and morphological changes in lungs of mice infected with Paracoccidioides brasiliensis. The animals were inoculated with 0.1 mL of fungal suspension of the P. brasiliensis 18 isolate and were euthanized 1, 2, 4 and 8 weeks after inoculation. The upper left lobe of the lung was isolated, fixed and processed for paraffin embedding. The sections were stained with H&E for histopathological study, with Gomori-Grocott to locate and identify the fungus, and with TUNEL immunostaining to detect the occurrence of programmed cell death. The lower and middle right lobes were analyzed by Fourier Transform Infrared Photoacoustic Spectrocopy (FTIR-PAS) to investigate physical and chemical features of the infected lungs. The results showed that lungs infected by P. brasiliensis underwent structural changes that varied according to the time period analyzed, and that changes in the absorption bands of different chemical groups resulted from these morphological changes. The results suggest that the combination of FTIR-PAS spectroscopy with morphological evaluation is an effective procedure for the study of paracoccidioidomycosis, one of the most important systemic mycoses that can damage the lung architecture and consequently impair the respiratory function.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Morphological and Structural Changes in Lung Tissue Infected by Paracoccidioides brasiliensis: FTIR Photoacoustic Spectroscopy and Histological Analysis

Morato

Morais

Sato

et al. 2013

Photochem & Photobiology

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“…Potential applications of such fiber probes attached to optical cables range from noninvasive diagnostics to skin treatment. The FTIR-FEW method used has been described in detail by Afanasyeva and coworkers 6 . In brief a typical experimental setup consists of a FTIR spectrometer, nitrogencooled mercury cadmium telluride (MCT) detector, (MIR) fiber optics, and fiber probes that operate in the attenuated total reflection (ATR) regime 36 .…”

Section: Biomedical Spectroscopy and The Ftir-few Spectroscopy Methodsmentioning

confidence: 99%

“…In fact lipids, proteins, nucleic acids and water are common constituents of human tissue [5][6][7][8][9][10][11][12] . The specific biochemical changes that occur in tumors are also dependent on the environment and location of cancer [13][14][15][16][17][18][19] .…”

Section: Introductionmentioning

confidence: 99%

<title>Chemical factor analysis of skin cancer FTIR-FEW spectroscopic data</title>

Bruch

Sukuta

2002

SPIE Proceedings

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Chemical Factor Analysis (CFA) algorithms were applied to transform complex Fourier transform infrared fiberoptical evanescent wave (FTIR-FEW) normal and malignant skin tissue spectra into factor spaces for analysis and classification. The factor space approach classified melanoma beyond prior pathological classifications related to specific biochemical alterations to health states in cluster diagrams allowing diagnosis with more biochemical specificity, resolving biochemical component spectra and employing health state eigenvector angular configurations as disease state sensors. This study demonstrated a wealth of new information from in vivo FTIR-FEW spectral tissue data, without extensive a priori information or clinically invasive procedures. In particular, we employed a variety of methods used in CFA to select the rank of spectroscopic data sets of normal benign and cancerous skin tissue. We used the Malinowski indicator function (IND), significance level (%MSL), and F-Tests to rank our data matrices. Normal skin tissue, melanoma and benign tumors were modeled by four, two and seven principal abstract factors, respectively. We also showed that the spectrum of the first eigenvalue was equivalent to the mean spectrum. The graphical depiction of angular disparities between the first abstract factors can be adopted as a new way to characterize and diagnose melanoma cancer.

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“…FTIR‐FEWS technique is based on the phenomenon of attenuated total reflection (ATR) and on IR transparent optical fibres which serve as a wave guide for the IR light. This technique was found useful in industrial (Afanasyeva et al , 2000) and biomedical fields (Bindig et al , 2001; Ring et al , 2006). In the medical fields FEWS was studied for the detection of cancerous tissues (Goldberg et al , 2004; Ring et al , 2006), but in most cases the obtained results were not conclusive due to low signal‐to‐noise ratio compared to results obtained in transmission mode using an IR microscope (Rave & Katzir, 2002).…”

Section: Introductionmentioning

confidence: 99%

Potential of ‘flat’ fibre evanescent wave spectroscopy to discriminate between normal and malignant cells in vitro

Hammody

Huleihel

Salman

et al. 2007

Journal of Microscopy

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SummaryThe present study focuses on evaluating the potential of flattened AgClBr fibre-optic evanescent wave spectroscopy (FTIR-FEWS) technique for detection and identification of cancer cells in vitro using cell culture as a model system. The FTIR-FEWS results are compared to those from FTIRmicrospectroscopy (FTIR-MSP) method extensively used to identify spectral properties of intact cells. Ten different samples of control and malignant cells were measured in parallel by the above two methods. Our results show a significant similarity between the results obtained by the two methodologies. The absorbance level of Amide I/Amide II, phosphates and carbohydrates were significantly altered in malignant compared to the normal cells using both systems. Thus, common biomarkers such as Amide I/Amide II, phosphate and carbohydrate levels can be derived to discern between normal and cancer cells. However, marked differences are also noted between the two methodologies in the protein bands due to CH 3 bending vibration (1480-1350 cm −1 ). The spectral differences may be attributed to the variation in the penetration depth of the two methodologies. The use of flattened fibre rather than the standard cylindrical fibre has several practical advantages and is considered as an important step towards in vivo measurements in real time, such as that of skin nevi and melanoma using special designs of fibre-optic-based sensors.

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<title>Numerous applications of fiber optic evanescent wave Fourier transform infrared (FEW-FTIR) spectroscopy for subsurface structural analysis</title>

Cited by 6 publications

References 17 publications

Morphological and Structural Changes in Lung Tissue Infected by Paracoccidioides brasiliensis: FTIR Photoacoustic Spectroscopy and Histological Analysis

Morphological and Structural Changes in Lung Tissue Infected by Paracoccidioides brasiliensis: FTIR Photoacoustic Spectroscopy and Histological Analysis

<title>Chemical factor analysis of skin cancer FTIR-FEW spectroscopic data</title>

Potential of ‘flat’ fibre evanescent wave spectroscopy to discriminate between normal and malignant cells in vitro

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