&lt;title&gt;The examinations of microorganisms by correlation optics method&lt;/title&gt;

Bilyi, Olexander I.

doi:10.1117/12.560055

Cited by 2 publications

(2 citation statements)

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“…It is common knowledge that index of refraction is a physical parameter, which determine light scattering properties of examined particles. Systematic refractometric analysis of bacterial suspension showed [20,21] that it is incorrect to speak about absolute refraction index value of bacteria from given species, as there is data spread of this values within given bacterial population. The cell of any nature consists of the following component: -nucleus, cytoplasm and plasma cell membrane, with different refraction indexes.…”

Section: Bacterial Cells Monitoring By Light Scattering Methodmentioning

confidence: 99%

Rapid detection of bacterial cells by light scattering method

et al. 2008

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Method of rapid detection of bacterial cells by light scattering is described. Determination of quantitative changes of bacteria is the given method based on the changes of their size distributing in the process of cultivation. Liquid medial are diluted and analyzed by the proposed technology to determine presence of bacteria. A method includes sounding of flow suspended bacterial cells by monochromatic coherent light, registration of signals of co-operation of sounding radiation with the explored microbiological objects by detects amplitudes and durations of scattered light impulses. Distribution of particles by sizes is determined from the measured functional dependence of number of registered particles from amplitude and duration of the proper electric impulses on the output photoreceiver. Detection is done for a range of particle size from 0.1 to 10 mkm, and thus particle's size distribution is determined. The results of studying of rapid detection of Escherichia coli by light scattering are described.

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Section: Bacterial Cells Monitoring By Light Scattering Methodmentioning

confidence: 99%

Rapid detection of bacterial cells by light scattering method

et al. 2008

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“…It is common knowledge that ndex of refraction is a physical parameter, which determine light scattering properties of examined particles. Systematic refractometric analysis of bacterial suspension showed [10,11] that it is incorrect to speak about absolute refraction index value of bacteria from given species, as there is data spread of this values within given bacterial population. Distribution curve for different bacterial species by the value of refraction index has normal pattern.…”

Section: Bacterial Cells Monitoring By Light Scattering Methodmentioning

confidence: 99%

Light scattering application for bacterial cell monitoring during cultivation process

Kotsyumbas

Kushnir

Bilyy

et al. 2007

Novel Optical Instrumentation for Biomedical Applications III

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Monitoring of bacterial cell numbers is of great importance not only in microbiological industry but also for control of liquids contamination in the food and pharmaceutical industries. Here we describe a novel low-cost and highly efficient technology for bacterial cell monitoring during cultivation process. The technology incorporates previously developed monitoring device and algorithm of its action. The devise analyses light scattered by suspended bacterial cells. Current stage utilizes monochromatic coherent light and detects amplitudes and durations of scattered light impulses, it does not require any labeling of bacterial cell. The system is calibrated using highly purificated bacteria-free water as standard. Liquid medial are diluted and analyzed by the proposed technology to determine presence of bacteria. Detection is done for a range of particle size from 0.1 to 10 um, and thus particles size distribution is determined. We analyzed a set of different bacterial suspensions and also their changes in quantity and size distribution during cultivation. Based on the obtained results we conclude that proposed technology can be very effective for bacteria monitoring during cultivation process, providing benefits of low simplicity and low cost of analysis with simultaneous high detection precision.

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<title>The examinations of microorganisms by correlation optics method</title>

Cited by 2 publications

References 0 publications

Rapid detection of bacterial cells by light scattering method

Rapid detection of bacterial cells by light scattering method

Light scattering application for bacterial cell monitoring during cultivation process

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