<title>Time-resolved fluorescence spectroscopy of tetrasulfonated aluminum phthalocyanine in solution and cells</title>

Abstract

Tetrasulphonated aluminum phthalocyanine (A1PcS4) is one of the extensively studied second generation sensitizers for photodynamic therapy of cancer. We've applied picosecond fluorescence spectroscopy to study the fluorescence decay lifetimes of A1PcS4 in phosphate buffer at different pH values and in monolayers of cultured cells incubated in photosensitizer solution. The decay time for the cells in the buffer is more close to the one obtained for acidic aqueous solution indicating that at least part ofthe phthalocyanine could be found in the acidic microenviroment (lysosomes, etc.).

Imaging of singlet oxygen feedback delayed fluorescence and lysosome permeabilization in tumor in vivo during photodynamic therapy with aluminum phthalocyanine

“…Although there may be a systematic bias up to ∼0.5 ns due to the relatively broad laser pulse (3 ns) and gate width (3 ns), these values are in line with the previously reported PF lifetimes in solutions (5 to 6 ns) and cells (4 to 5 ns). 42,43,48 PF lifetime in acidic pH 4.1 was reported to be shorter by 0.5 ns, when compared to neutral pH 7. 48 This is one of the possible reasons for the PF lifetime increase after lysosomal permeabilization, because the interior of lysosomes is known to be more acidic than cytosol.…”

<title>Pecularities of clinical photodynamic therapy of cancer</title>

“…The preparation is made synthetically from aluminium phthalocyanine. Physico-chemial properties, pharmacokinetics, mechanism of action and the results of experimental PDT with phthalocyanines are presented in a number ofpublications (3,4,5).…”