LUM is the hub gene of advanced fibrosis in nonalcoholic fatty liver disease patients

Chang, Yue; He, Jiange; Xiang, Xiao-Hui; Li, Hai

doi:10.1016/j.clinre.2020.04.006

Cited by 10 publications

(6 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In liver fibrosis, lumican regulates the assembly of collagen fibers and activates TGF-β and SMA. Moreover, upregulated lumican expression is observed in patients with non-alcoholic fatty liver disease and non-alcoholic steatohepatitis, substantiating its role in liver fibrosis ( Charlton et al ., 2009 ; Barbariga et al ., 2019 ; Mohammadzadeh et al ., 2019 ; Chang et al ., 2021 ). In our study, Il1r2 and Irak3 emerged as the most strongly affected genes following PHMG-p treatment.…”

Section: Discussionmentioning

confidence: 99%

A New Murine Liver Fibrosis Model Induced by Polyhexamethylene Guanidine-Phosphate

Kim

Hur

Kim

et al. 2021

Biomol Ther (Seoul)

View full text Add to dashboard Cite

Liver fibrosis is part of the wound healing process to help the liver recover from the injuries caused by various liver-damaging insults. However, liver fibrosis often progresses to life-threatening cirrhosis and hepatocellular carcinoma. To overcome the limitations of current in vivo liver fibrosis models for studying the pathophysiology of liver fibrosis and establishing effective treatment strategies, we developed a new mouse model of liver fibrosis using polyhexamethylene guanidine phosphate (PHMG-p), a humidifier sterilizer known to induce lung fibrosis in humans. Male C57/BL6 mice were intraperitoneally injected with PHMG-p (0.03% and 0.1%) twice a week for 5 weeks. Subsequently, liver tissues were examined histologically and RNA-sequencing was performed to evaluate the expression of key genes and pathways affected by PHMG-p. PHMG-p injection resulted in body weight loss of ~15% and worsening of physical condition. Necropsy revealed diffuse fibrotic lesions in the liver with no effect on the lungs. Histology, collagen staining, immunohistochemistry for smooth muscle actin and collagen, and polymerase chain reaction analysis of fibrotic genes revealed that PHMG-p induced liver fibrosis in the peri-central, peri-portal, and capsule regions. RNA-sequencing revealed that PHMG-p affected several pathways associated with human liver fibrosis, especially with upregulation of lumican and IRAK3, and downregulation of GSTp1 and GSTp2, which are closely involved in liver fibrosis pathogenesis. Collectively we demonstrated that the PHMG-p-induced liver fibrosis model can be employed to study human liver fibrosis.

show abstract

Section: Discussionmentioning

confidence: 99%

A New Murine Liver Fibrosis Model Induced by Polyhexamethylene Guanidine-Phosphate

Kim

Hur

Kim

et al. 2021

Biomol Ther (Seoul)

View full text Add to dashboard Cite

show abstract

“…LUM and DCN exert influence on ECM composition, thereby modulating the fibrotic progression. It was found that elevated LUM levels observed in the hepatic tissue and serum of individuals afflicted with advanced fibrosis suggest the potential utility of LUM as a diagnostic biomarker for assessing the severity of NAFLD liver fibrosis [ 38 ]. DCN operates by sequestering transforming growth factor β (TGFβ), a pivotal profibrogenic cytokine, thereby impeding its activity.…”

Section: Discussionmentioning

confidence: 99%

Integrative proteomics and metabolomics explore the effect and mechanism of Qiyin granules on improving nonalcoholic fatty liver disease

Yan,

Liu,

Huang

et al. 2024

Heliyon

View full text Add to dashboard Cite

“…The levels of type III collagen formation and degradation were significantly increased in ALD patients compared to healthy individuals ( 46 ). In addition, LUM has been identified as a biomarker for advanced fibrosis in non-alcoholic fatty liver disease ( 47 ). THBS2 is a novel biomarker for predicting the prognosis of metastatic pancreatic ductal adenocarcinoma ( 48 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of biomarkers co-associated with M1 macrophages, ferroptosis and cuproptosis in alcoholic hepatitis by bioinformatics and experimental verification

et al. 2023

View full text Add to dashboard Cite

BackgroundsAlcoholic hepatitis (AH) is a major health problem worldwide. There is increasing evidence that immune cells, iron metabolism and copper metabolism play important roles in the development of AH. We aimed to explore biomarkers that are co-associated with M1 macrophages, ferroptosis and cuproptosis in AH patients.MethodsGSE28619 and GSE103580 datasets were integrated, CIBERSORT algorithm was used to analyze the infiltration of 22 types of immune cells and GSVA algorithm was used to calculate ferroptosis and cuproptosis scores. Using the “WGCNA” R package, we established a gene co-expression network and analyzed the correlation between M1 macrophages, ferroptosis and cuproptosis scores and module characteristic genes. Subsequently, candidate genes were screened by WGCNA and differential expression gene analysis. The LASSO-SVM analysis was used to identify biomarkers co-associated with M1 macrophages, ferroptosis and cuproptosis. Finally, we validated these potential biomarkers using GEO datasets (GSE155907, GSE142530 and GSE97234) and a mouse model of AH.ResultsThe infiltration level of M1 macrophages was significantly increased in AH patients. Ferroptosis and cuproptosis scores were also increased in AH patients. In addition, M1 macrophages, ferroptosis and cuproptosis were positively correlated with each other. Combining bioinformatics analysis with a mouse model of AH, we found that ALDOA, COL3A1, LUM, THBS2 and TIMP1 may be potential biomarkers co-associated with M1 macrophages, ferroptosis and cuproptosis in AH patients.ConclusionWe identified 5 potential biomarkers that are promising new targets for the treatment and diagnosis of AH patients.

show abstract

LUM is the hub gene of advanced fibrosis in nonalcoholic fatty liver disease patients

Cited by 10 publications

References 27 publications

A New Murine Liver Fibrosis Model Induced by Polyhexamethylene Guanidine-Phosphate

A New Murine Liver Fibrosis Model Induced by Polyhexamethylene Guanidine-Phosphate

Integrative proteomics and metabolomics explore the effect and mechanism of Qiyin granules on improving nonalcoholic fatty liver disease

Identification of biomarkers co-associated with M1 macrophages, ferroptosis and cuproptosis in alcoholic hepatitis by bioinformatics and experimental verification

Contact Info

Product

Resources

About