Luminance white noise electroretinograms (wnERGs) in mice

Stallwitz, Nina; Joachimsthaler, Anneka; Kremers, Jan

doi:10.3389/fnins.2022.1075126

Cited by 2 publications

(16 citation statements)

References 25 publications

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“…The detailed description of TWN stimulus can be found in our previous publication ( Stallwitz et al, 2022 ). Briefly, the TWN stimuli were created by modulating the luminance outputs of three differently colored light emitting diodes (LEDs).…”

Section: Methodsmentioning

confidence: 99%

“…In each animal, recordings were performed in four separate sessions: one session for luminance modulation as described in Stallwitz et al (2022) and three sessions for opsin-isolating stimuli described in the present study. The sessions were at least 1 week apart so that the animals could fully recover from a previous recording.…”

Section: Methodsmentioning

confidence: 99%

“…In vision research, this stimulus has been extensively used in recordings from single neurons ( Chichilnisky, 2001 ; Field et al, 2010 ). The TWN stimulus has been recently introduced in ERG measurements, to characterize ERG generating mechanisms in human subjects ( Saul and Still, 2016 ; Zele et al, 2017 ; Adhikari et al, 2019 ), monkeys ( Kremers et al, 2022 ) and mice ( Wang et al, 2019 ; Stallwitz et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…We previously investigated the ERG responses elicited by luminance TWN stimuli (white noise ERGs; wnERGs) in LIAIS mice ( Stallwitz et al, 2022 ). We studied the dependency of the IRFs and MTFs on mean luminance.…”

Section: Introductionmentioning

confidence: 99%

“…We studied the dependency of the IRFs and MTFs on mean luminance. We also studied the correlations between wnERGs obtained at identical measurements, describing the reproducibility of the wnERGs, and at different MLs, giving information about the underlying ERG generating mechanisms ( Stallwitz et al, 2022 ). In the present study, we extended these measurements by using photopigment-isolating TWN stimuli.…”

Section: Introductionmentioning

confidence: 99%

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Single opsin driven white noise ERGs in mice

Stallwitz,

Joachimsthaler,

Kremers

2023

Front. Neurosci.

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PurposeElectroretinograms elicited by photopigment isolating white noise stimuli (wnERGs) in mice were measured. The dependency of rod- and cone-opsin-driven wnERGs on mean luminance was studied.MethodsTemporal white noise stimuli (containing all frequencies up to 20 Hz, equal amplitudes, random phases) that modulated either rhodopsin, S-opsin or L*-opsin, using the double silent substitution technique, were used to record wnERGs in mice expressing a human L*-opsin instead of the native murine M-opsin. Responses were recorded at 4 mean luminances (MLs).Impulse response functions (IRFs) were obtained by cross-correlating the wnERG recordings with the corresponding modulation of the photopigment excitation elicited by the stimulus. So-called modulation transfer functions (MTFs) were obtained by performing a Fourier transform on the IRFs.Potentials of two repeated wnERG recordings at corresponding time points were plotted against each other. The correlation coefficient (r2repr) of the linear regression through these data was used to quantify reproducibility. Another correlation coefficient (r2ML) was used to quantify the correlations of the wnERGs obtained at different MLs with those at the highest (for cone isolating stimuli) or lowest (for rod isolating stimuli) ML.ResultsIRFs showed an initial negative (a-wave like) trough N1 and a subsequent positive (b-wave like) peak P1. No oscillatory potential-like components were observed. At 0.4 and 1.0 log cd/m2 ML robust L*- and S-opsin-driven IRFs were obtained that displayed similar latencies and dependencies on ML. L*-opsin-driven IRFs were 2.5–3 times larger than S-opsin-driven IRFs. Rhodopsin-driven IRFs were observed at −0.8 and − 0.2 log cd/m2 and decreased in amplitude with increasing ML. They displayed an additional pronounced late negativity (N2), which may be a correlate of retinal ganglion cell activity.R2repr and r2ML values increased for cones with increasing ML whereas they decreased for rods. For rhodopsin-driven MTFs at low MLs and L*-opsin-driven MTFs at high MLs amplitudes decreased with increasing frequency, with much faster decreasing amplitudes for rhodopsin. A delay was calculated from MTF phases showing larger delays for rhodopsin- vs. low delays for L*-opsin-driven responses.ConclusionOpsin-isolating wnERGs in mice show characteristics of different retinal cell types and their connected pathways.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Single opsin driven white noise ERGs in mice

Stallwitz,

Joachimsthaler,

Kremers

2023

Front. Neurosci.

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Melanopsin-mediated amplification of cone signals in the human visual cortex

Adhikari,

Uprety,

Feigl

et al. 2024

Proc. R. Soc. B.

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The ambient daylight variation is coded by melanopsin photoreceptors and their luxotonic activity increases towards midday when colour temperatures are cooler, and irradiances are higher. Although melanopsin and cone photoresponses can be mediated via separate pathways, the connectivity of melanopsin cells across all levels of the retina enables them to modify cone signals. The downstream effects of melanopsin-cone interactions on human vision are however, incompletely understood. Here, we determined how the change in daytime melanopsin activation affects the human cone pathway signals in the visual cortex. A 5-primary silent-substitution method was developed to evaluate the dependence of cone-mediated signals on melanopsin activation by spectrally tuning the lights and stabilizing the rhodopsin activation under a constant cone photometric luminance. The retinal (white noise electroretinogram) and cortical responses (visual evoked potential) were simultaneously recorded with the photoreceptor-directed lights in 10 observers. By increasing the melanopsin activation, a reverse response pattern was observed with cone signals being supressed in the retina by 27% ( p = 0.03) and subsequently amplified by 16% ( p = 0.01) as they reach the cortex. We infer that melanopsin activity can amplify cone signals at sites distal to retinal bipolar cells to cause a decrease in the psychophysical Weber fraction for cone vision.

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Luminance white noise electroretinograms (wnERGs) in mice

Cited by 2 publications

References 25 publications

Single opsin driven white noise ERGs in mice

Single opsin driven white noise ERGs in mice

Melanopsin-mediated amplification of cone signals in the human visual cortex

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