Luminescence decay kinetics in relation to the relaxation of the transthylakoid ΔpH from high and low CO<sub>2</sub> adapted cells of <i>Scenedesmus obliquus</i>

Sundblad, Lars-Göran; Palmqvist, Kristin; Samuelsson, Göran

doi:10.1016/0014-5793(86)81078-x

Cited by 9 publications

(6 citation statements)

References 17 publications

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“…As a consequence, reoxidation of PQ may well proceed at a high rate even though the rate of energy turnover through PSII has decreased (14). Taken together, our results support the idea that increased cyclic electron transport around PSI may provide the cells with extra ATP for the CCM (24,26,29), but apparently at the cost of a decreased noncyclic electron transport (Fig. 6).…”

Section: Low-ci-induced Changes Of Fluorescence Quenchingsupporting

confidence: 79%

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Photosynthetic Light Utilization Efficiency, Photosystem II Heterogeneity, and Fluorescence Quenching in Chlamydomonas reinhardtii during the Induction of the CO₂-Concentrating Mechanism

Falk

Palmqvist²

1992

Plant Physiol.

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The photosynthetic light-response curve, the relative amounts of the different photosystem II (PSII) units, and fluorescence quenching were altered in an adaptive manner when CO(2)-enriched wild-type Chlamydomonas reinhardtii cells were transferred to low levels of CO(2). This treatment is known to result in the induction of an energy-dependent CO(2)-concentrating mechanism (CCM) that increases the internal inorganic carbon concentration and thus the photosynthetic CO(2) utilization efficiency. After 3 to 6 h of low inorganic carbon treatment, several changes in the photosynthetic energy-transducing reactions appeared and proceeded for about 12 h. After this time, the fluorescence parameter variable/maximal fluorescence yield and the amounts of both PSIIalpha and PSIIbeta (secondary quinone electron acceptor of PSII-reducing) centers had decreased, whereas the amount of PSIIbeta (secondary quinone electron acceptor of PSII-nonreducing) centers had increased. The yield of noncyclic electron transport also decreased during the induction of the CCM, whereas both photochemical and nonphotochemical quenching of PSII fluorescence increased. Concurrent with these changes, the photosynthetic light-utilization efficiency also decreased significantly, largely attributed to a decline in the curvature parameter theta, the convexity of the photosynthetic light-response curve. Thus, it is concluded that the increased CO(2) utilization efficiency in algal cells possessing the CCM is maintained at the cost of a reduced light utilization efficiency, most probably due to the reduced energy flow through PSII.

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Section: Low-ci-induced Changes Of Fluorescence Quenchingsupporting

confidence: 79%

“…Several studies have dealt with changes in photosynthetic C02-response characteristics and changes in carbonic anhydrase levels that occur when algae are transferred from high to low Ci concentrations (2-4, 13, 17, 22, 23). However, there are few studies dealing with the regulation of the photosynthetic energy transducing reactions (24,26,29) …”

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confidence: 99%

Photosynthetic Light Utilization Efficiency, Photosystem II Heterogeneity, and Fluorescence Quenching in Chlamydomonas reinhardtii during the Induction of the CO₂-Concentrating Mechanism

Falk

Palmqvist²

1992

Plant Physiol.

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“…In Sundblad et al (1986b) the luminescence maximum from low CO2 adapted Scenedesmus was shown to be correlated to a rapid relaxation of nonphotochemical quenching of maximal fluorescence. It was concluded that the luminescence maximum appeared as a result of a rapid energetic turnover in low CO2 adapted cells and that the luminescence maximum and the rapid relaxation of nonphotochemical quenching reflected a rapid ApH relaxation.…”

Section: Resultsmentioning

confidence: 99%

Luminescence decay kinetics in relation to quenching and stimulation of dark fluorescence from high and low CO2 adapted cells of Scenedesmus obliquus and Chlamydomonas reinhardtii

et al. 1990

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Two green algal species, Chlamydomonas reinhardtii and Scenedesmus obliquus, exhibited a relative maximum during the decay of luminescence, when adapted to low CO2 conditions that was not observed in high CO2 adapted cells.From the kinetics of transient changes in the level of dark fluorescence, after illumination and parallel to the luminescence maxima, it was concluded that the maximum in Scenedesmus was mainly related to a decrease in nonphotochemical quenching, whereas in Chlamydomonas the maximum was mainly related to a dark reduction of the primary PS II acceptor QA.ATP/ADP ratios from low CO2 adapted Scenedesmus showed transient high levels after a dark/light transition that was not observed in high CO2 adapted cells. After 30 s of illumination the ATP/ADP ratios however stabilized at the same steady state level as in high CO2 adapted cells.Dark addition of HCO3 (-) to low CO2 adapted cells of Chlamydomonas resulted in a rapid transient quenching of luminescence that was not observed in low CO2 adapted cells of neither species.It is concluded that the luminescence maxima present in both low CO2 adapted Scenedesmus and Chlamydomonas reflect adaptation of the cells to low CO2 conditions. It is further suggested that the difference in mechanistic origin of luminescence maxima in the two species reflects differences in adaptation.

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“…An I AG /I B ratio of 1.17 has been determined in L. japonicus plants grown under air conditions (Table ). As already mentioned, the intensity of AG emission is considered as an in vivo indicator of the assimilatory potential (NADPH + ATP) when it is inducible by a white light illumination in green algae (Mellvig and Tillberg , Palmqvist et al , Sundblad et al ) or flash excitation in plants (Miranda and Ducruet , Krieger et al ). The assimilatory potential, which is also dependent on the cellular energetic status, has also been correlated to the dihydroxyacetone‐P/PGA ratio (Heber et al ).…”

Section: Discussionmentioning

confidence: 99%

The afterglow thermoluminescence band as an indicator of changes in the photorespiratory metabolism of the model legumeLotus japonicus

García‐Calderón

Betti

Márquez

et al. 2019

Physiologia Plantarum

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The afterglow (AG) luminescence is a delayed chlorophyll fluorescence emitted by the photosystem II that seems to reflect the level of assimilatory potential (NADPH+ATP) in chloroplast. In this work, the thermoluminescence (TL) emissions corresponding to the AG band were investigated in plants of the WT and the Ljgln2‐2 photorespiratory mutant from Lotus japonicus grown under either photorespiratory (air) or non‐photorespiratory (high concentration of CO2) conditions. TL glow curves obtained after two flashes induced the strongest overall TL emissions, which could be decomposed in two components: B band (tmax = 27–29°C) and AG band (tmax = 44–45°C). Under photorespiratory conditions, WT plants showed a ratio of 1.17 between the intensity of the AG and B bands (IAG/IB). This ratio increased considerably under non‐photorespiratory conditions (2.12). In contrast, mutant Ljgln2‐2 plants grown under both conditions showed a high IAG/IB ratio, similar to that of WT plants grown under non‐photorespiratory conditions. In addition, high temperature thermoluminescence (HTL) emissions associated to lipid peroxidation were also recorded. WT and Ljgln2‐2 mutant plants grown under photorespiratory conditions showed both a significant HTL band, which increased significantly under non‐photorespiratory conditions. The results of this work indicate that changes in the amplitude of IAG/IB ratio could be used as an in vivo indicator of alteration in the level of photorespiratory metabolism in L. japonicus chloroplasts. Moreover, the HTL results suggest that photorespiration plays some role in the protection of the chloroplast against lipid peroxidation.

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Luminescence decay kinetics in relation to the relaxation of the transthylakoid ΔpH from high and low CO₂ adapted cells of Scenedesmus obliquus

Cited by 9 publications

References 17 publications

Photosynthetic Light Utilization Efficiency, Photosystem II Heterogeneity, and Fluorescence Quenching in Chlamydomonas reinhardtii during the Induction of the CO₂-Concentrating Mechanism

Photosynthetic Light Utilization Efficiency, Photosystem II Heterogeneity, and Fluorescence Quenching in Chlamydomonas reinhardtii during the Induction of the CO₂-Concentrating Mechanism

Luminescence decay kinetics in relation to quenching and stimulation of dark fluorescence from high and low CO2 adapted cells of Scenedesmus obliquus and Chlamydomonas reinhardtii

The afterglow thermoluminescence band as an indicator of changes in the photorespiratory metabolism of the model legumeLotus japonicus

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Luminescence decay kinetics in relation to the relaxation of the transthylakoid ΔpH from high and low CO2 adapted cells of Scenedesmus obliquus

Cited by 9 publications

References 17 publications

Photosynthetic Light Utilization Efficiency, Photosystem II Heterogeneity, and Fluorescence Quenching in Chlamydomonas reinhardtii during the Induction of the CO2-Concentrating Mechanism

Photosynthetic Light Utilization Efficiency, Photosystem II Heterogeneity, and Fluorescence Quenching in Chlamydomonas reinhardtii during the Induction of the CO2-Concentrating Mechanism

Luminescence decay kinetics in relation to quenching and stimulation of dark fluorescence from high and low CO2 adapted cells of Scenedesmus obliquus and Chlamydomonas reinhardtii

The afterglow thermoluminescence band as an indicator of changes in the photorespiratory metabolism of the model legumeLotus japonicus

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Luminescence decay kinetics in relation to the relaxation of the transthylakoid ΔpH from high and low CO₂ adapted cells of Scenedesmus obliquus

Photosynthetic Light Utilization Efficiency, Photosystem II Heterogeneity, and Fluorescence Quenching in Chlamydomonas reinhardtii during the Induction of the CO₂-Concentrating Mechanism

Photosynthetic Light Utilization Efficiency, Photosystem II Heterogeneity, and Fluorescence Quenching in Chlamydomonas reinhardtii during the Induction of the CO₂-Concentrating Mechanism