Luminescence method for detection of aflatoxin B1 using ATP-releasing nucleotides

Hu, Dongyue; Xiao, Shusen; Guo, Qiaqia; Yue, Rongrong; Geng, Demin; Ji, Debin

doi:10.1039/d1ra03870b

Cited by 3 publications

(1 citation statement)

References 27 publications

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“…Multiplex and real-time PCR assays have been developed for this purpose, and they target genes, including aflD(nor), omtA, aflM(ver), aflR, and aflJ [40]. Hu and colleagues (2021) developed a novel luminescence detection method that employs ATP-releasing nucleotides (ARNs) and AFB1 aptamer for AFB1 detection [41]. The authors synthesized two ARNs (dTP 4 A and dGP 4 A) without using ATP as the starting material, and the method provided a lower detection limit of 0.3 pM.…”

Section: Molecular-based Techniquesmentioning

confidence: 99%

Aflatoxin Contamination: An Overview on Health Issues, Detection and Management Strategies

Alameri

Kong

Aljaafari

et al. 2023

Toxins

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Aflatoxins (AFs) represent one of the main mycotoxins produced by Aspergillus flavus and Aspergillus parasiticus, with the most prevalent and lethal subtypes being AFB1, AFB2, AFG1, and AFG2. AFs are responsible for causing significant public health issues and economic concerns that affect consumers and farmers globally. Chronic exposure to AFs has been linked to liver cancer, oxidative stress, and fetal growth abnormalities among other health-related risks. Although there are various technologies, such as physical, chemical, and biological controls that have been employed to alleviate the toxic effects of AF, there is still no clearly elucidated universal method available to reduce AF levels in food and feed; the only mitigation is early detection of the toxin in the management of AF contamination. Numerous detection methods, including cultures, molecular techniques, immunochemical, electrochemical immunosensor, chromatographic, and spectroscopic means, are used to determine AF contamination in agricultural products. Recent research has shown that incorporating crops with higher resistance, such as sorghum, into animal feed can reduce the risk of AF contamination in milk and cheese. This review provides a current overview of the health-related risks of chronic dietary AF exposure, recent detection techniques, and management strategies to guide future researchers in developing better detection and management strategies for this toxin.

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Section: Molecular-based Techniquesmentioning

confidence: 99%

Aflatoxin Contamination: An Overview on Health Issues, Detection and Management Strategies

Alameri

Kong

Aljaafari

et al. 2023

Toxins

View full text Add to dashboard Cite

show abstract

An efficient aggregation-induced electrochemiluminescent immunosensor by using TiO2 nanoparticles as coreaction accelerator and energy donor for aflatoxin B1 detection

Miao

et al. 2022

Anal Bioanal Chem

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A fluorescent aptasensor based on nitrogen-doped carbon supported palladium and exonuclease III-assisted signal amplification for sensitive detection of AFB1

Zhao

Suo

et al. 2022

Analytica Chimica Acta

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Luminescence method for detection of aflatoxin B1 using ATP-releasing nucleotides

Abstract: Schematic illustration of a luminescence short DNA sequence detection method using ATP-releasing nucleotides. Combined with AFB1 aptamer, this method is used to detect AFB1.

Cited by 3 publications

References 27 publications

Aflatoxin Contamination: An Overview on Health Issues, Detection and Management Strategies

Aflatoxin Contamination: An Overview on Health Issues, Detection and Management Strategies

An efficient aggregation-induced electrochemiluminescent immunosensor by using TiO2 nanoparticles as coreaction accelerator and energy donor for aflatoxin B1 detection

A fluorescent aptasensor based on nitrogen-doped carbon supported palladium and exonuclease III-assisted signal amplification for sensitive detection of AFB1

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