Introduction In ZnSe the prominent Y line at 2.6 eV has been attributed to extended defects [1][2][3]. Very recently the radiative recombination of an exciton bound to a dissociated 60 a-type glide dislocation has been revealed as origin of the emission. Structural differences of the particular dislocation core have been associated with a spectral splitting of the Y line, which gives rise to a characteristic peak series [4]. The current understanding of the strain relaxation processes in mismatched heterostructures is ascribed to the models of Matthews-Blakeslee [5] and DodsonTsao [6], respectively. In the first one, threading dislocations breaking through the heterointerface and ending up in the layer surface are assumed to be an essential precondition for the relaxation process. In contrast, dislocation multiplication is required in the framework of the Dodson-Tsao model. Dislocation nucleation at the surface of the epilayer has been considered by various authors [7,8]. Surface nucleation of dislocation half-loops starts when the layer thickness exceeds a critical value. Subsequent strain driven propagation and interaction of dislocations cause major plastic relaxation and realize a final misfit dislocation network. Preferential formation of dissociated dislocations aligned in ½1 1 10 have been observed by transmission electron microscope investigations in epitaxially grown (001) ZnSe films [9], just above the critical layer thickness. During this transient relaxation state, the appearing layer stress is clearly anisotropic [8].In the present letter we report on cathodoluminescence studies analyzing lateral differences of the spectral splitting of the Y line in a thin ZnSe film. The Y line originates most frequently from 2 mm wide dislocated areas, exclusively aligned collinearly to ½1 1 10, differing locally by the number and peak energies of individual Y line subcomponents. We explain our findings by the formation of dislocation bundles consisting of parallel and dissociated a glide dislocations. These dislocation bundles are supposed to be generated by surface nucleation and slid into the interior of the ZnSe film.