Luminescent silicon nanoparticles for distinctive tracking of cellular targeting and trafficking

Kim, Gi‐Heon; Lee, Goun; Kang, Myoung Hee; Kim, Minjong; Jin, Yusung; Beck, Sungjun; Cheon, Jihyun; Sung, Junyeong; Joo, Jinmyoung

doi:10.1039/c9fd00124g

Cited by 9 publications

(4 citation statements)

References 66 publications

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“… 33 − 36 SiNPs with excellent biocompatibility have also been successfully applied in cell imaging and cell tracking. 37 , 38 The above reports indicated that SiNPs have excellent application potential in the field of analytical detection. So far, the fluorescence method based on SiNPs for VB 2 detection has not been reported.…”

Section: Introductionmentioning

confidence: 98%

“…As novel fluorescent probes, SiNPs have attracted the interest of researchers. In recent years, SiNPs have been used to detect metal ions such as Cu 2+ , Ag + , Fe 3+ , Hg 2+ , and Mn 2+ ; − tetracycline antibiotic drugs such as tetracycline and oxytetracycline; , and essential nutrients for human body like vitamins, amino acids, glucose, and cholesterol. − SiNPs with excellent biocompatibility have also been successfully applied in cell imaging and cell tracking. , The above reports indicated that SiNPs have excellent application potential in the field of analytical detection. So far, the fluorescence method based on SiNPs for VB 2 detection has not been reported.…”

Section: Introductionmentioning

confidence: 99%

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Silicon Nanoparticle-Based Ratiometric Fluorescence Probes for Highly Sensitive and Visual Detection of VB₂

Pan

Qin

et al. 2023

ACS Omega

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In this work, blue fluorescent silicon nanoparticles (SiNPs) were prepared by a simple one-step hydrothermal method using (3-aminopropyl) triethoxy silane (APTES) and eriochrome black T as raw materials. The SiNPs showed favorable water solubility, thermal stability, pH stability, salt tolerance, and photobleaching resistance. At an excitation wavelength of 376 nm, the SiNPs emitted bright blue fluorescence at 460 nm. In the presence of vitamin B2 (VB2), the fluorescence intensity (FL intensity) of the SiNPs at 460 nm decreased obviously, and a new peak appeared at 521 nm. Based on this, a novel ratiometric fluorescence method was established for VB2 detection. There was a good linear relationship between the fluorescence intensity ratio (F 521/F 460) and VB2 concentration from 0.5 to 60 μM with a detection limit of 135 nM. This method was successfully applied to detect VB2 content in the samples of vitamin B2 drugs and beverages. Additionally, a simple paper sensor based on the SiNPs was designed to visualize detection of VB2. With the support of color recognition software on a smartphone, the visual quantitative analysis of VB2 was realized, ranging from 40 to 800 μM.

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Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Silicon Nanoparticle-Based Ratiometric Fluorescence Probes for Highly Sensitive and Visual Detection of VB₂

Pan

Qin

et al. 2023

ACS Omega

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“…Lately, fluorescent probes based on SiNPs were successfully used to detect heavy metal ions, , dopamine, amino acids, vitamins, nitro explosives, etc. Furthermore, SiNPs could also be used for pH sensing and bioimaging. ,− Nevertheless, the application of SiNPs in the detection of active ingredient of Chinese medicine is relatively rare.…”

Section: Introductionmentioning

confidence: 99%

Water Soluble Silicon Nanoparticles as a Fluorescent Probe for Highly Sensitive Detection of Rutin

Pan

Qin

et al. 2022

ACS Omega

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In this work, water-soluble fluorescent silicon nanoparticles (SiNPs) were prepared by one-pot hydrothermal method using 3-(2-aminoethylamino)propyldimethoxymethylsilane (AEAPDMMS) as a silicon source and amidol as a reducing agent. The prepared SiNPs showed bright green fluorescence, excellent stability against photobleaching, salt tolerance, temperature stability, and good water solubility. Due to the internal filtration effect (IFE), rutin could selectively quench the fluorescence of the SiNPs. Based on such phenomena, a highly sensitive fluorescence method was established for rutin detection. The linear range and limit of detection (LOD) were 0.05–400 μM and 15.2 nM, respectively. This method was successfully applied to detect rutin in the samples of rutin tablets, Sophora japonica , fry Sophora japonica , and S. japonica carbon with satisfactory recovery.

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“…During the past few years, taking advantage of the low toxicity of silicon, fluorescent SiNPs have been extensively explored as prime candidates for biocompatible fluorescent nanostructures, holding tremendous promise for biosensing and bioimaging applications. Specifically, fluorescent SiNPs have been shown to be superbly suitable for use in long-term and real-time imaging, cellular targeting and trafficking, fluorescence ink, the detection and photodynamic treatment of bacterial infections, and cancer cell detection and therapy [ 25 , 26 , 27 , 28 , 29 , 30 ]. There have been advances in the understanding of the toxicity of SiNPs in both cell cultures and mammalian animal models, but these knowledges are still fragmented and sometimes inconsistent.…”

Section: Introductionmentioning

confidence: 99%

The In Vivo Toxicity Assessments of Water-Dispersed Fluorescent Silicon Nanoparticles in Caenorhabditis elegans

Wang

Zhu

Song

et al. 2022

IJERPH

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Fluorescent silicon nanoparticles (SiNPs), resembling a typical zero-dimensional silicon nanomaterial, have shown great potential in a wide range of biological and biomedical applications. However, information regarding the toxicity of this material in live organisms is still very scarce. In this study, we utilized Caenorhabditis elegans (C. elegans), a simple but biologically and anatomically well-described model, as a platform to systematically investigate the in vivo toxicity of SiNPs in live organisms at the whole-animal, cellular, subcellular, and molecular levels. We calculated the effect of SiNPs on C. elegans body length (N ≥ 75), lifespan (N ≥ 30), reproductive capacity (N ≥ 10), endocytic sorting (N ≥ 20), endoplasmic reticulum (ER) stress (N ≥ 20), mitochondrial stress (N ≥ 20), oxidative stress (N ≥ 20), immune response (N ≥ 20), apoptosis (N ≥ 200), hypoxia response (N ≥ 200), metal detoxification (N ≥ 200), and aging (N ≥ 200). The studies showed that SiNPs had no significant effect on development, lifespan, or reproductive ability (p > 0.05), even when the worms were treated with a high concentration (e.g., 50 mg/mL) of SiNPs at all growth and development stages. Subcellular analysis of the SiNP-treated worms revealed that the intracellular processes of the C. elegans intestine were not disturbed by the presence of SiNPs (p > 0.05). Toxicity analyses at the molecular level also demonstrated that the SiNPs did not induce harmful or defensive cellular events, such as ER stress, mitochondria stress, or oxidative stress (p > 0.05). Together, these findings confirmed that the SiNPs are low in toxicity and biocompatible, supporting the suggestion that the material is an ideal fluorescent nanoprobe for wide-ranging biological and biomedical applications.

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Luminescent silicon nanoparticles for distinctive tracking of cellular targeting and trafficking

Abstract: We demonstrate tracking of silicon nanoparticles through intrinsic photoluminescence during the course of cellular targeting and uptake.

Cited by 9 publications

References 66 publications

Silicon Nanoparticle-Based Ratiometric Fluorescence Probes for Highly Sensitive and Visual Detection of VB₂

Silicon Nanoparticle-Based Ratiometric Fluorescence Probes for Highly Sensitive and Visual Detection of VB₂

Water Soluble Silicon Nanoparticles as a Fluorescent Probe for Highly Sensitive Detection of Rutin

The In Vivo Toxicity Assessments of Water-Dispersed Fluorescent Silicon Nanoparticles in Caenorhabditis elegans

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Luminescent silicon nanoparticles for distinctive tracking of cellular targeting and trafficking

Abstract: We demonstrate tracking of silicon nanoparticles through intrinsic photoluminescence during the course of cellular targeting and uptake.

Cited by 9 publications

References 66 publications

Silicon Nanoparticle-Based Ratiometric Fluorescence Probes for Highly Sensitive and Visual Detection of VB2

Silicon Nanoparticle-Based Ratiometric Fluorescence Probes for Highly Sensitive and Visual Detection of VB2

Water Soluble Silicon Nanoparticles as a Fluorescent Probe for Highly Sensitive Detection of Rutin

The In Vivo Toxicity Assessments of Water-Dispersed Fluorescent Silicon Nanoparticles in Caenorhabditis elegans

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Silicon Nanoparticle-Based Ratiometric Fluorescence Probes for Highly Sensitive and Visual Detection of VB₂

Silicon Nanoparticle-Based Ratiometric Fluorescence Probes for Highly Sensitive and Visual Detection of VB₂