Luminol‐enhanced chemiluminescence after reaction of hydroperoxides with opsonized zymosan

Heinle, H.; Dessouki, Jasmin El

doi:10.1002/bio.1170100202

Cited by 7 publications

(5 citation statements)

References 9 publications

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“…Despite the known cytotoxic effects of DMSO, no adverse effects on LDCL were observed at the concentration of 1% (v/v) used in this study, indicating safety and applicability of low DMSO concentrations towards bovine blood and milk PMN functional assays. This finding is also in agreement with a previous study in human PMN (37). Contrarily, at concentrations higher than 1% (v/v), adverse effects of DMSO on LDCL of both blood and milk PMN were not surprising.…”

Section: Discussionsupporting

confidence: 93%

A comparative study of bovine blood and milk neutrophil functions with luminol‐dependent chemiluminescence

et al. 2001

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In this study, a technique was developed for the chemiluminescence (CL) measurement of bovine milk polymorphonuclear leukocytes (PMN). In the first study, the effects of cell number and the concentration of phorbol-12-myristate-13-acetate (PMA), luminol, latex bead particles, dimethyl sulphoxide (DMSO) and gelatin on the luminol-dependent cellular CL (LDCL) response were assessed with healthy cows in different stages of lactation. In the second study, the LDCL and in vitro bactericidal activity of blood and milk PMN towards Staphylococcus aureus was investigated. In general, the CL activity of blood PMN was consistently higher than that of milk PMN. We found that (a) the optimal cell density in blood and milk cells for maximal LDCL response ranged from 1.5 x 10(6) to 5 x 10(6) cells/mL; (b) the optimal concentrations of PMA, latex beads and luminol for maximal LDCL response were 100-200 ng/ml, 500 particles/PMN and 0.1 mmol/L, respectively. Concentrations of DMSO of 0.5-1% (v/v) did not significantly affect the maximal CL response of PMN. Gelatin concentrations of 0.1 -0.5 mg/ml had no effect on the LDCL of PMN. In addition, the LDCL of PMN was significantly correlated with bactericidal activity towards S. aureus (r = 0.78, p < 0.001 for blood PMN and r = 0.66, p < 0.01 for milk PMN). Under the optimal experimental conditions for measurement of CL produced by bovine blood and milk PMN defined in this study, LDCL assay is an accurate and reproducible technique for the rapid quantification of PMN bactericidal activity in physiological and pathological conditions of high-yielding dairy cows.

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Section: Discussionsupporting

confidence: 93%

A comparative study of bovine blood and milk neutrophil functions with luminol‐dependent chemiluminescence

et al. 2001

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“…The amount of hydroperoxide formation in the early stages of photo-degradation of polypropylene, which was one of the RDF components in the present study, was determined using chemiluminescence (George & Ghaemy 1991, Ghaemy & George 1993, Kron et al 1996. Moreover, the chemiluminescence method has been commonly used to study the oxidation of various substances involved in hydroperoxides, including low-density polyethylene (Catalina et al 2002), oxidative stress in Alzheimer patients (Repetto et al 1999), lipids (Benov & Ribarov 1990), and characterization of zymosan-induced phagocytosis (Heinle & Dessouki 1995).…”

Section: Resultsmentioning

confidence: 89%

Investigation on the spontaneous combustion of refuse-derived fuels during storage using a chemiluminescence technique

et al. 2008

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Refuse-derived fuel (RDF), a high-caloric material, is used by various combustion processes, such as power plants, as alternative fuel. Several explosion accidents, however, possibly initiated by the spontaneous combustion of stored RDF, have been reported in Japan. Therefore the spontaneous combustion of RDF prepared from domestic garbage was investigated using chemiluminescence. RDF samples were heated either under air or under nitrogen for 1, 2, or 4 h at 120 or 140 degrees C and then cooled by an air or nitrogen stream. All RDF samples exhibited chemiluminescence. In air-treated RDF samples (heated and cooled by air), chemiluminescence after ageing was shown to be slightly lower than before ageing, whereas in nitrogen-treated samples (both heated and cooled by nitrogen) chemiluminescence decreased significantly after ageing. When nitrogen was replaced with air during aging, however, a sudden increase of chemiluminescence was observed. On the other hand, when cooling was done with air, chemiluminescence increased. Higher chemiluminescence was also observed during high-temperature treatment. Further experiments on cellulose, one of the major components of domestic garbage, exhibited similar chemiluminescence patterns to those of RDF when treated by the same methods as those used for RDF ageing. Chemiluminescence from cellulose increased significantly when the atmospheric gas was changed from nitrogen to air, suggesting that oxygen in the air promoted the formation of hydroperoxide from cellulose. Therefore, it is hypothesized that cellulose plays an important role in the formation of chemiluminescence from RDF. The formation of chemiluminescence indicated that radicals are formed from RDF by oxidation or thermal degradation at room or atmospheric temperatures and may subsequently lead to spontaneous combustion.

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“…The potent inhibitor of cellular superoxide production NDGA [41] shows the same antioxidant effect as the steroid compounds mentioned above.…”

Section: Discussionmentioning

confidence: 87%

Systematic Investigation of Different Steroid Precursors with Respect to their Effect on Superoxide Anion Production by Human Neutrophil Granulocytes

2004

Horm Metab Res

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Free radicals are involved in several pathological processes in living organisms, for example in athero- and oncogenesis. Some steroids are known to be effective antioxidants, while others do not play any such role. The aim of our study was to examine the antioxidant capability of different metabolites in the synthesis of steroid hormones. As a model, we chose human neutrophils producing superoxide anion, which is the source of many other radicals. Neutrophils were separated from healthy volunteers. Isolated cells were incubated with varying concentrations of steroid compounds and stimulated with N-formyl-Met-Leu-Phe. Superoxide anion production was determined by photometry. Neutrophils incubated with corticosterone and 18-hydroxy-deoxycorticosterone showed a significant reduction in superoxide production, whereas we found a significant enhancement in the presence of 11beta-hydroxyprogesterone. Furthermore, we observed a non-significant decreasing trend after incubation with cholesterol 3-sulphate and an increasing tendency using 11-hydroxyandrostenedione. We were also able to produce newer morphological and functional evidence of the role of myeloperoxidase enzyme in the steroidal antioxidant effect by electronic microscopy and use of sodium hypochlorite in our incubation model. Based on these results, we conclude that not only steroid end products but also their intermediate metabolites, most of which are also present in human plasma, partly influence free radical metabolism. Thus, this study provides further argument for the search for the molecular basis responsible for the antioxidant effect of steroid structures. This may lead to new opportunities for finding really efficient antioxidants, which might perhaps be used in a combined manner with other agents in the fight against certain life-threatening diseases.

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Luminol‐enhanced chemiluminescence after reaction of hydroperoxides with opsonized zymosan

Cited by 7 publications

References 9 publications

A comparative study of bovine blood and milk neutrophil functions with luminol‐dependent chemiluminescence

A comparative study of bovine blood and milk neutrophil functions with luminol‐dependent chemiluminescence

Investigation on the spontaneous combustion of refuse-derived fuels during storage using a chemiluminescence technique

Systematic Investigation of Different Steroid Precursors with Respect to their Effect on Superoxide Anion Production by Human Neutrophil Granulocytes

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