Lunasin protease inhibitor concentrate decreases pro-inflammatory cytokines and improves histopathological markers in dextran sodium sulfate-induced ulcerative colitis

Nieto-Veloza, Andrea; Wang, Zhihong; Zhong, Qixin; D’Souza, Doris H.; Krishnan, Hari B.; Dia, Vermont P.

doi:10.1016/j.fshw.2022.06.008

Cited by 11 publications

(11 citation statements)

References 35 publications

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“…Caspase-1 gene-deficient mice significantly reduced their susceptibility to UC by inhibiting the secretion of pro-inflammatory cytokines IL-1β and IL-18 . IL-1β induces the synthesis of inflammatory factors (e.g., IL-6) and acts synergistically with IL-17 and IL-18 to mediate intestinal inflammatory responses. , In this study, serum levels of IL-1β, IL-6, TNF-α, IL-17A, and IL-18 were elevated in the DSS-fed mice. Some polyphenolic compounds (such as chlorogenic acid and wogonoside) have been shown to inhibit the NLRP3 inflammasome, thus preventing colitis. , In our previous study, SA could inhibit the NLRP3 cascade reaction and inflammation in DSS-fed mice with colitis.…”

Section: Discussionmentioning

confidence: 49%

“…41 IL-1β induces the synthesis of inflammatory factors (e.g., IL-6) and acts synergistically with IL-17 and IL-18 to mediate intestinal inflammatory responses. 42,43 In this study, serum levels of IL-1β, IL-6, TNF-α, IL-17A, and IL-18 were elevated in the DSSfed mice. Some polyphenolic compounds (such as chlorogenic acid and wogonoside) have been shown to inhibit the NLRP3 inflammasome, thus preventing colitis.…”

Section: Discussionmentioning

confidence: 49%

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Sinapic Acid Attenuates Chronic DSS-Induced Intestinal Fibrosis in C57BL/6J Mice by Modulating NLRP3 Inflammasome Activation and the Autophagy Pathway

Li,

Liu,

Wang

et al. 2023

ACS Omega

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Ulcerative colitis (UC) is a chronic gastrointestinal disease that results from repeated inflammation and serious complications. Sinapic acid (SA) is a hydroxycinnamic acid present in a variety of plants that has antioxidant, antiinflammatory, anticancer, and other protective effects. This study investigated the antifibrotic effect of SA on chronic colitis induced by dextran sulfate sodium salt (DSS) in mice. We observed that SA could significantly reduce clinical symptoms (such as improved body weight loss, increased colon length, and decreased disease activity index score) and pathological changes in mice with chronic colitis. SA supplementation has been demonstrated to repair intestinal mucosal barrier function and maintain epithelial homeostasis by inhibiting activation of the NLRP3 inflammasome and decreasing the expression of IL-6, TNF-α, IL-17A, IL-18, and IL-1β. Furthermore, SA could induce the expression of antioxidant enzymes (Cat, Sod1, Sod2, Mgst1) by activating the Nrf2/keap1 pathway, thus improving antioxidant capacity. Additionally, SA could increase the protein expression of downstream LC3-II/LC3-I and Beclin1 and induce autophagy by regulating the AMPK-Akt/mTOR signaling pathway, thereby reducing the production of intestinal fibrosis-associated proteins Collagen-I and α-SMA. These findings suggest that SA can enhance intestinal antioxidant enzymes, reduce oxidative stress, expedite intestinal epithelial repair, and promote autophagy, thereby ameliorating DSS-induced colitis and intestinal fibrosis.

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Section: Discussionmentioning

confidence: 49%

Section: Discussionmentioning

confidence: 49%

Sinapic Acid Attenuates Chronic DSS-Induced Intestinal Fibrosis in C57BL/6J Mice by Modulating NLRP3 Inflammasome Activation and the Autophagy Pathway

Li,

Liu,

Wang

et al. 2023

ACS Omega

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“…NO is a potent pro‐inflammatory mediator that studies have reported its increased levels in the inflamed tissue of the colon. It has an important role in the production of inflammatory cytokines in relevant areas 35 . On the other hand, damage to the colon, increases due to increased levels of NO and, following that, reactive nitrogenous species, and reactive oxygen species 36 …”

Section: Discussionmentioning

confidence: 99%

“…It has an important role in the production of inflammatory cytokines in relevant areas. 35 On the other hand, damage to the colon, increases due to increased levels of NO and, following that, reactive nitrogenous species, and reactive oxygen species. 36 It indicates the importance of NF-κB-INOS-NO signaling pathways in imposing oxidative stress and inflammation due to colitis.…”

Section: Discussionmentioning

confidence: 99%

Quinic acid ameliorates ulcerative colitis in rats, through the inhibition of two TLR4‐NF‐κB and NF‐κB‐INOS‐NO signaling pathways

Ghasemi‐Dehnoo

Lorigooini

Amini‐Khoei

et al. 2023

Immunity Inflam & Disease

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ObjectiveIn this study, the therapeutic effect of quinic acid (QA), which has anti‐inflammatory activity, was investigated on acetic acid‐induced colitis in male Wistar rats.MethodsUlcerative colitis (UC) was induced in rats by acetic acid intrarectally, and the protective effects of QA in 10, 30, 60, and 100 mg/kg doses were investigated. Rats were treated for 5 days and their colon tissues were dissected out at the end. Macroscopic and histopathological examinations were performed in colon tissues. Also, the expression of inflammatory and apoptotic genes, including TLR4, IL‐1β, INOS, IL‐6, TNF‐α, NF‐κB, Caspase‐3, Caspase‐8, Bax, and Bcl‐2, was measured. Biochemistry indices, such as malondialdehyde (MDA) and nitrite oxide (NO) content, in addition to, total antioxidant capacity (TAC), superoxide dismutase (SOD), catalase (CAT), and enzymes activities were also assessed.ResultsColitis increased the levels of MDA and NO, and enhanced the inflammatory and apoptotic gene expressions, while reducing the SOD and CAT enzymes activity, and TAC levels in the colitis rats. Also, results showed that colitis was associated with the infiltration of inflammatory cells, epithelium damage, and edema in colon tissue. QA significantly ameliorated histopathological indices, oxidative stress, inflammation, and apoptosis in colitis rats.ConclusionQA ameliorated UC through the inhibition of two TLR4‐NF‐κB and NF‐κB‐INOS‐NO signaling pathways, which results in the reduction of colitis complications, including oxidative stress, inflammation, apoptosis and histopathological injuries in rats. Therefore it can be concluded, that QA exerts its therapeutic effects through antiapoptotic, antioxidant, and anti‐inflammatory properties.

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“…[ 17 ]. They can block the activity of trypsin and chymotrypsin in the gastrointestinal system, protect against the hydrolysis of dietary functional proteins and peptides [ 18 , 19 ], and have anti-cancer, anti-inflammatory, anti-bacterial, and hypoglycemic functional properties [ 20 , 21 , 22 ]. Studies have shown that the coadministration of soybean trypsin inhibitor with teriparatide has a significant effect on the oral absorption of teriparatide [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Interactions between Soybean Trypsin Inhibitor and Chitosan in an Aqueous Solution

Zhang

Liu

et al. 2023

Polymers

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Supramolecular structures obtained from protein–polysaccharide association may be applied to encapsulate bioactive compounds or to improve the physical stability and texture properties of colloid–based products. In this study, the interaction of 0.1 wt% soybean trypsin inhibitor (STI) with different concentrations of chitosan (CS) in aqueous solutions was investigated under different pH by the analysis of state diagram, turbidity, zeta potential, spectroscopy, and microstructure; the protective effect of STI–CS complex coacervates on STI stability in simulated gastric juice was also discussed. The results suggested that interactions between STI and CS could form soluble/insoluble complexes mainly through hydrophobic interactions (pH 4.0) or electrostatic interactions (pH 6.0). The CD spectra showed that the secondary structure of STI did not change significantly when CS with the same charge was mixed with STI, and the secondary structure of STI was slightly changed when CS with the opposite charge was mixed with STI. Simulated gastric digestion experiments showed that the complex formed by non-covalent bonding had a protective effect on the active protein. This study provides information about the effect of different CS concentrations and pH values on the formation of complexes of CS and STI in an aqueous solution and provides theoretical references for the construction of supramolecular-structured carrier substances based on CS and STI.

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Lunasin protease inhibitor concentrate decreases pro-inflammatory cytokines and improves histopathological markers in dextran sodium sulfate-induced ulcerative colitis

Cited by 11 publications

References 35 publications

Sinapic Acid Attenuates Chronic DSS-Induced Intestinal Fibrosis in C57BL/6J Mice by Modulating NLRP3 Inflammasome Activation and the Autophagy Pathway

Sinapic Acid Attenuates Chronic DSS-Induced Intestinal Fibrosis in C57BL/6J Mice by Modulating NLRP3 Inflammasome Activation and the Autophagy Pathway

Quinic acid ameliorates ulcerative colitis in rats, through the inhibition of two TLR4‐NF‐κB and NF‐κB‐INOS‐NO signaling pathways

Interactions between Soybean Trypsin Inhibitor and Chitosan in an Aqueous Solution

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