Luteimonas arsenica sp. nov., an arsenic-tolerant bacterium isolated from arsenic-contaminated soil

Mu, Yao; Pan, Yunfan; Shi, Wanxia; Liu, Lan; Zhao, Jiuran; Luo, Xuesong; Zeng, Xian‐Chun; Li, Wenjun

doi:10.1099/ijsem.0.001024

Cited by 35 publications

(13 citation statements)

References 29 publications

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“…and Comamonadaceae (Methylibium sp.) were associated with the conventional farming system in our dataset, which have been previously used in the bioremediation of hydrocarboncontaminated soil (Kane et al, 2007;Mu et al, 2016). The latter can also induce disease resistance through the production of antimicrobial compounds (Lagos et al, 2015).…”

Section: Impact Of Maize Populations and Farming System On The Associmentioning

confidence: 99%

Effect of Low-Input Organic and Conventional Farming Systems on Maize Rhizosphere in Two Portuguese Open-Pollinated Varieties (OPV), “Pigarro” (Improved Landrace) and “SinPre” (a Composite Cross Population)

et al. 2021

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Maize is one of the most important crops worldwide and is the number one arable crop in Portugal. A transition from the conventional farming system to organic agriculture requires optimization of cultivars and management, the interaction of plant–soil rhizosphere microbiota being pivotal. The objectives of this study were to unravel the effect of population genotype and farming system on microbial communities in the rhizosphere of maize. Rhizosphere soil samples of two open-pollinated maize populations (“SinPre” and “Pigarro”) cultivated under conventional and organic farming systems were taken during flowering and analyzed by next-generation sequencing (NGS). Phenological data were collected from the replicated field trial. A total of 266 fungi and 317 bacteria genera were identified in “SinPre” and “Pigarro” populations, of which 186 (69.9%) and 277 (87.4%) were shared among them. The microbiota of “Pigarro” showed a significant higher (P < 0.05) average abundance than the microbiota of “SinPre.” The farming system had a statistically significant impact (P < 0.05) on the soil rhizosphere microbiota, and several fungal and bacterial taxa were found to be farming system-specific. The rhizosphere microbiota diversity in the organic farming system was higher than that in the conventional system for both varieties. The presence of arbuscular mycorrhizae (Glomeromycota) was mainly detected in the microbiota of the “SinPre” population under the organic farming systems and very rare under conventional systems. A detailed metagenome function prediction was performed. At the fungal level, pathotroph–saprotroph and pathotroph–symbiotroph lifestyles were modified by the farming system. For bacterial microbiota, the main functions altered by the farming system were membrane transport, transcription, translation, cell motility, and signal transduction. This study allowed identifying groups of microorganisms known for their role as plant growth-promoting rhizobacteria (PGPR) and with the capacity to improve crop tolerance for stress conditions, allowing to minimize the use of synthetic fertilizers and pesticides. Arbuscular mycorrhizae (phyla Glomeromycota) were among the most important functional groups in the fungal microbiota and Achromobacter, Burkholderia, Erwinia, Lysinibacillus, Paenibacillus, Pseudomonas, and Stenotrophomonas in the bacterial microbiota. In this perspective, the potential role of these microorganisms will be explored in future research.

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Section: Impact Of Maize Populations and Farming System On The Associmentioning

confidence: 99%

Effect of Low-Input Organic and Conventional Farming Systems on Maize Rhizosphere in Two Portuguese Open-Pollinated Varieties (OPV), “Pigarro” (Improved Landrace) and “SinPre” (a Composite Cross Population)

et al. 2021

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“…Luteimonas species can be non-motile (L. pelagia) [23] or motile (L. abyssi, L. mephitis, L. notoginsengisoli, L. rhizosphaerae, L. terrae and L. yindakuii) [24][25][26][27][28][29]. These two isolates were motile as tested in semisolid BHI agar, an observation supported by the physical presence of a single polar flagellum ( Fig.…”

Section: Luteimonas Chenhongjianii a Novel Species Isolated From Recmentioning

confidence: 75%

“…Among other findings from analysis of the newly generated genomes, strain 100111 T carries a gene encoding ArsH, an arsenical resistance protein, a finding in line with seven genomes of other Luteimonas species with validly published names (L. abyssi, L. aestuarii, L. arsenica, L. huabeiensis, L. mephitis, L. terrae, L. terricola). Notably, L. arsenica was isolated from arsenic-contaminated soil, capable of resisting arsenate and arsenite [28], and it remains to be seen whether the arsH gene is functional in strains 100111 T and 100069.…”

Section: Luteimonas Chenhongjianii a Novel Species Isolated From Recmentioning

confidence: 99%

“…Within the tested range of 0.5-8.5 % NaCl (w/v; at 1.0 % intervals), strains 100069 and 100111 T could grow in BHI broth with 0.5-3.5 % NaCl (w/v; optimum, 0.5 %). In BHI broth of different pH (pH 3.0-11.0, at intervals of 1.0 pH units) as buffered with citrate/phosphate buffer or Tris/hydrochloride buffer after 5 days of incubation at 37 °C [34], strains 100069 and 100111 T grew between pH 6.0-10.0 (optimum, pH 7.0-8.0 [23,28,[34][35][36][37][38][39][40]. The low ANI value (76.93 %) between strain 100111 T and L. yindakuii can distinguish these two species.…”

Section: Luteimonas Chenhongjianii a Novel Species Isolated From Recmentioning

confidence: 99%

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Luteimonas chenhongjianii, a novel species isolated from rectal contents of Tibetan Plateau pika (Ochotona curzoniae)

Gui

Lai

Yang

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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Two Gram-stain-negative, strictly aerobic, bright-yellow-pigmented and rod-shaped bacteria (strains 100069 and 100111T) with a single polar flagellum were isolated from the rectal contents of plateau pika (Ochotona curzoniae). Based on the results of nearly full-length 16S rRNA gene sequence and phylogenetic analyses, strains 100069 and 100111T belong to the genus Luteimonas , and are closest to Luteimonas rhizosphaerae 4-12T (98.02 % similarity), Luteimonas aestuarii B9T (97.8 %) and Luteimonas terrae THG-MD21T (97.74 %). The DNA G+C contents of these two isolates were 68.30 mol% and 68.29 mol%, respectively. The highest average nucleotide identity (ANI) value between strain 100111T and its closely related species was 83.34 %, well below the threshold of 95–96 %. The major cellular fatty acids were iso-C11 : 0, iso-C15 : 0 and iso-C17 : 1 ω9. Polar lipid content was dominated by diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified lipid. Ubiquinone-8 (Q-8) was the predominant respiratory quinone. These two isolates grew optimally at 35–37 °C, pH 7.0–8.0 and with 1.0 % (w/v) NaCl. The results of ANI analysis and other characteristics obtained from our polyphasic study showed that strains 100069 and 100111T represent a novel species in genus Luteimonas , for which the name Luteimonas chenhongjianii sp. nov. (type strain 100111T=DSM 104077T=CGMCC 1.16429T) is proposed.

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“…A protein sequence homology search against the GenBank database was performed using the BLAST server. Multiple sequence alignments were conducted using the ClustalW2 software as described elsewhere ( Zhang et al, 2015 ; Mu et al, 2016a ). A phylogenetic tree of the obtained ArsMs and their closely related homologues was constructed using the maximum likelihood method implemented in MEGA 6.0 as described previously ( Xu et al, 2014 ; Mu et al, 2016b ).…”

Section: Methodsmentioning

confidence: 99%

Microbially Mediated Methylation of Arsenic in the Arsenic-Rich Soils and Sediments of Jianghan Plain

et al. 2018

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Almost nothing is known about the activities and diversities of microbial communities involved in As methylation in arsenic-rich shallow and deep sediments; the correlations between As biomethylation and environmental parameters also remain to be elucidated. To address these issues, we collected 9 arsenic-rich soil/sediment samples from the depths of 1, 30, 65, 95, 114, 135, 175, 200, and 223 m in Jianghan Plain, China. We used microcosm assays to determine the As-methylating activities of the microbial communities in the samples. To exclude false negative results, we amended the microcosms with 0.2 mM As(III) and 20.0 mM lactate. The results indicated that the microbial communities in all of the samples significantly catalyzed arsenic methylation. The arsM genes were detectable from all the samples with the exception of 175 m, and 90 different arsM genes were identified. All of these genes code for new or new-type ArsM proteins, suggesting that new As-methylating microorganisms are widely distributed in the samples from shallow to deep sediments. To determine whether microbial biomethylation of As occurs in the sediments under natural geochemical conditions, we conducted microcosm assays without exogenous As and carbons. After 80.0 days of incubation, approximately 4.5–15.5 μg/L DMAsV were detected in all of the microcosms with the exception of that from 30 m, and 2.0–9.0 μg/L MMAsV were detected in the microcosms of 65, 114, 135, 175, 200, and 223 m; moreover, approximately 18.7–151.5 μg/L soluble As(V) were detected from the nine sediment samples. This suggests that approximately 5.3, 0, 8.1, 28.9, 18.0, 8.7, 13.8, 10.2, and 14.9% of total dissolved As were methylated by the microbial communities in the sediment samples from 1, 30, 65, 95, 114, 135, 175, 200, and 223 m, respectively. The concentrations of biogenic DMAsV show significant positive correlations with the depths of sediments, and negative correlations with the environmental NH4+ and NaCl concentrations, but show no significant correlations with other environmental parameters, such as NO3-, SO42+, TOC, TON, Fe, Sb, Cu, K, Ca, Mg, Mn, and Al. This work helps to better understand the biogeochemical cycles of arsenic in arsenic-rich shallow and deep sediments.

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Luteimonas arsenica sp. nov., an arsenic-tolerant bacterium isolated from arsenic-contaminated soil

Cited by 35 publications

References 29 publications

Effect of Low-Input Organic and Conventional Farming Systems on Maize Rhizosphere in Two Portuguese Open-Pollinated Varieties (OPV), “Pigarro” (Improved Landrace) and “SinPre” (a Composite Cross Population)

Effect of Low-Input Organic and Conventional Farming Systems on Maize Rhizosphere in Two Portuguese Open-Pollinated Varieties (OPV), “Pigarro” (Improved Landrace) and “SinPre” (a Composite Cross Population)

Luteimonas chenhongjianii, a novel species isolated from rectal contents of Tibetan Plateau pika (Ochotona curzoniae)

Microbially Mediated Methylation of Arsenic in the Arsenic-Rich Soils and Sediments of Jianghan Plain

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