Luteolin Arrests Cell Cycling, Induces Apoptosis and Inhibits the JAK/STAT3 Pathway in Human Cholangiocarcinoma Cells

Aneknan, Ploypailin; Kukongviriyapan, Veerapol; Prawan, Auemduan; Kongpetch, Sarinya; Sripa, Banchob; Senggunprai, Laddawan

doi:10.7314/apjcp.2014.15.12.5071

Cited by 46 publications

(36 citation statements)

References 25 publications

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“…They mediate anticancer effects with selective toxicity towards cancer cells [16]. The antitumor effect of flavonoids including luteolin, quercetin, and EGCG in CCA are mediated through the suppression of JAK/STAT signaling [9,17] and induction of mitochondrial cell death pathway [18]. In the present study, candidone showed a cytotoxic effect against two CCA cells, KKU-M156 and KKU-M213, in the micromolar range.…”

Section: Discussionsupporting

confidence: 49%

“…The scratch wound healing assay was employed to evaluate cell migration as previously described [9]. Briefly, KKU-M156 cells were seeded into a 24-well plate at a density of 250,000 cells/well and allowed to grow for 24 h. An opened wound was scratched using a sterile 200 μL pipette tip.…”

Section: Wound Healing Assaymentioning

confidence: 99%

“…For example, apigenin exhibits antiproliferation and induces apoptosis in renal carcinoma [8]. In CCA, luteolin suppresses the proliferation and migration of CCA cell lines [9].…”

Section: Introductionmentioning

confidence: 99%

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Antitumor effects of candidone extracted from <i>Derris indica</i> (Lamk) Bennet in cholangiocarcinoma cells

Kurasug¹,

Kukongviriyapan²,

Prawan³

et al. 2018

Trop. J. Pharm Res

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Section: Discussionsupporting

confidence: 49%

Section: Wound Healing Assaymentioning

confidence: 99%

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Antitumor effects of candidone extracted from <i>Derris indica</i> (Lamk) Bennet in cholangiocarcinoma cells

Kurasug¹,

Kukongviriyapan²,

Prawan³

et al. 2018

Trop. J. Pharm Res

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“…Cells were cultured at 37˚C in a 5% CO 2 incubator for another 10 days to obtain cell colonies and the Ham's F-12 complete medium was freshly renewed every 3 days. On the last experiment day, the cell colonies were fixed with absolute methanol at room temperature (25˚C) for 20 min, stained with 0.5% crystal violet solution at room temperature (25˚C) for 15 min and the number of cell colonies was counted (23).…”

Section: Methodsmentioning

confidence: 99%

Downregulation of NAD(P)H:quinone oxidoreductase 1 inhibits proliferation, cell cycle and migration of cholangiocarcinoma cells

et al. 2017

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Abstract. We previously reported that upregulation of NAD(P)H:quinone oxidoreductase 1 (NQO1) in cholangiocarcinoma (CCA; a fatal bile duct cancer) was associated with poor prognosis. It was also demonstrated that the suppression of NQO1 was able to enhance the chemosensitivity of CCA cells. In the present study, in order to elucidate the biological role of NQO1 in CCA, the effects of small interfering RNA (siRNA)-mediated knockdown of NQO1 on cell proliferation, cell cycle and migration were determined in KKU-100 CCA cells, which notably expressed NQO1. The cell proliferation ability and cell cycle distribution were identified by clonogenic cell survival assay and flow cytometric analysis, respectively. Wound healing and Transwell migration assays were performed to evaluate cell migration. The molecules involved in cell proliferation and migration were determined by western blot analysis and reverse transcription-quantitative polymerase chain reaction analysis. The results demonstrated that NQO1 siRNA-mediated knockdown effectively impaired colony formation capacity, induced cell cycle arrest at the G 1 phase and suppressed migration of KKU-100 cells. CCA cells transfected with NQO1 siRNA exhibited increased expression levels of p21 and decreased cyclin D1 protein expression levels. Furthermore, the ratio of matrix metalloproteinase 9/tissue inhibitors of metalloproteinases 1 (TIMP1) mRNA expression level was decreased in the NQO1-knockdown cells. Therefore, the present study provided evidence supporting the biological role of NQO1 in the regulation of cell proliferation, cell cycle and migration of CCA cells. Therefore, NQO1 may prove to be a potential molecular target to enhance CCA treatment.

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“…In a separate study, suppression of IL-6 mediated pSTAT3 reduced colony forming ability and promoted cell-cycle arrest in CCA cell lines [92].…”

Section: Accepted Manuscriptmentioning

confidence: 93%

Pathogenesis of cholangiocarcinoma: From genetics to signalling pathways

Kongpetch

Jusakul

Ong

et al. 2015

Best Practice & Research Clinical Gastroenterology

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Luteolin Arrests Cell Cycling, Induces Apoptosis and Inhibits the JAK/STAT3 Pathway in Human Cholangiocarcinoma Cells

Cited by 46 publications

References 25 publications

Antitumor effects of candidone extracted from <i>Derris indica</i> (Lamk) Bennet in cholangiocarcinoma cells

Antitumor effects of candidone extracted from <i>Derris indica</i> (Lamk) Bennet in cholangiocarcinoma cells

Downregulation of NAD(P)H:quinone oxidoreductase 1 inhibits proliferation, cell cycle and migration of cholangiocarcinoma cells

Pathogenesis of cholangiocarcinoma: From genetics to signalling pathways

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