Luteotropic and Luteolytic Effects of Peptides in the Porcine and Human Corpus Luteum

Wuttke, W.; Jarry, Hubertus; Pitzel, L.; Dietrich, Eva-Maria; Spieß, S.

doi:10.1007/978-1-4613-8336-9_13

Cited by 7 publications

(6 citation statements)

References 17 publications

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“…There is much evidence for a systemic role of OT in the control of luteal function, in which there is a positive feedback loop at luteolysis between luteal OT and endometrial prostaglandin (PG) F2α [6]. On the other hand, a local effect of luteal OT on steroidogenesis has previously been suggested in cow [7,8], sheep [8] and swine [9,10]. The local role of luteal OT has been confirmed by the finding of specific binding sites for OT in the cyclic bovine CL [11,12].…”

Section: Luteotropic Role Of Luteal Oxytocinmentioning

confidence: 99%

Luteotropic Mechanisms in the Bovine Corpus Luteum: Role of Oxytocin, Prostaglandin F2 α, Progesterone and Noradrenaline

Skarżyński

Jaroszewski

Okuda

2001

Journal of Reproduction and Development

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Abstract. The main function of the corpus luteum (CL) is production of progesterone (P4). Adequate luteal function to secrete P4 is crucial for determining the physiological duration of the estrous cycle and for achieving a successful pregnancy. The bovine CL grows very fast and regresses within two days at luteolysis. The mechanism controlling the development and secretory function of the bovine CL may involve many factors that are produced both within and outside the CL. Some of these regulators seem to be prostaglandins (PGs), oxytocin (OT) and adrenergic factors such as noradrenaline (NA). Recently, there has been some evidence that P4 acts within the bovine CL as an autocrine and/or paracrine regulator. Each of these factors may act on the CL independently or may modify the actions of others. The purpose of this paper is to review and discuss the possible roles of OT, PGs, P4 and NA as luteotropic auto/paracrine regulators in the bovine CL. Furthermore, intraluteal mechanisms controlling the sensitivity of the CL to extragonadal PGF2α are discussed.

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Section: Luteotropic Role Of Luteal Oxytocinmentioning

confidence: 99%

Luteotropic Mechanisms in the Bovine Corpus Luteum: Role of Oxytocin, Prostaglandin F2 α, Progesterone and Noradrenaline

Skarżyński

Jaroszewski

Okuda

2001

Journal of Reproduction and Development

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“…gesterone production [7]. TNF␣ administration by microdialysis within the CL induced functional luteal regression in pigs [8]. It has been suggested that an increase in the number of TNF receptors or a sudden release of TNF␣ may inhibit the luteotropic action of LH and induce luteal regression by inhibition of adenyl cyclase [3].…”

Section: Introductionmentioning

confidence: 98%

Macrophages Are the Major Source of Tumor Necrosis Factor α in the Porcine Corpus Luteum1

Zhao

Burbach

Roby

et al. 1998

Biology of Reproduction

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This study was designed to determine the source of tumor necrosis factor (TNF) alpha within the porcine corpus luteum (CL). 1) Sections of frozen or paraffin-embedded CL from various stages of the estrous cycle were incubated with the following primary antibodies: anti-human recombinant TNFalpha, anti-porcine macrophage-specific antigen, or anti-alpha-actin (marker of pericyte and smooth muscle cells). Dolichos biflorus lectin-peroxidase was used as an endothelial cell label. Positive immunostaining for TNFalpha was apparent in porcine CL throughout the estrous cycle. TNFalpha immunoreactivity was primarily localized in cells along septal/vascular tracts, and exhibited spatial and temporal distribution similar to that of cells labeled with anti-macrophage antibodies. Large luteal cells exhibited weak staining for TNFalpha in paraffin sections, whereas microvascular endothelial cells were consistently negative in both frozen and paraffin sections. 2) Enriched subpopulations of macrophages, endothelial cells, and large and small luteal cells were isolated by density gradient and immunomagnetic bead separation techniques. TNFalpha secretion by each subpopulation was determined by measuring bioactive TNFalpha in incubation media using a specific in vitro bioassay. Macrophage subpopulations secreted up to 100-fold greater quantities of bioactive TNFalpha (up to 400 pg/10(6) cells) than did other subpopulations. In contrast, endothelial cell and small luteal cell subpopulations released very small amounts (< 8 pg/10(6) cells) of bioactive TNFalpha. Large luteal cells secreted slightly greater amounts of TNFalpha (10-15 pg/10(6) cells). Local macrophages appear to be the primary source of TNFalpha in the porcine CL.

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“…Oxytocin (OT) and oxytocin receptors (OTRs) are both expressed in human and subhuman primate ovaries [1][2][3][4][5][6], and it has been suggested that the local production of OT is important in regulating ovarian function [7,8]. More specifically, OT may be a paracrine mediator of luteinization in primate ovary [5,6].…”

Section: Introductionmentioning

confidence: 99%

Oxytocin Receptor Regulation and Action in a Human Granulosa-Lutein Cell Line1

Copland

Zlatnik

Ives

et al. 2002

Biology of Reproduction

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Although oxytocin and its receptor have been identified in human ovary, its regulatory role in granulosa cell or corpus luteum function has not been clearly defined. To better understand oxytocin action in the human ovary, we have characterized the expression and function of oxytocin receptors in an immortalized human granulosa-lutein cell line, HGL5. Expression of oxytocin receptor mRNA was demonstrated by reverse transcriptase-polymerase chain reaction analysis, and by specific binding of an iodinated oxytocin antagonist (apparent dissociation constant of 131 +/- 0.15 pM, and a B(max) of 12 +/- 0.5 fmol/microg DNA). Receptor levels were down-regulated by serum starvation, and rapidly up-regulated by serum restoration. Stimulation of protein kinase C activity increased oxytocin receptor levels in a concentration-dependent manner. Conversely, protein kinase C inhibition blocked up-regulation of oxytocin receptors. Treatment of cells with 10 nM oxytocin resulted in a rapid, transient increase in intracellular Ca(2+), and the response was blocked by an oxytocin antagonist. Because HGL5 cells secrete progesterone and estradiol in response to agents that elevate intracellular cAMP concentrations, we studied the effect of oxytocin on steroid production. Oxytocin enhanced the effects of forskolin on progesterone production. These results suggest that oxytocin augments the activity of luteotropins in vivo. Our studies are the first to show an ovarian cell line that expresses functional oxytocin receptors. These cells can serve as a useful model for studying oxytocin signal pathways and their cross-talk with respect to progesterone synthesis. These cells also will be useful in the analysis of mechanisms of oxytocin receptor regulation, including regulation of its gene.

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Luteotropic and Luteolytic Effects of Peptides in the Porcine and Human Corpus Luteum

Cited by 7 publications

References 17 publications

Luteotropic Mechanisms in the Bovine Corpus Luteum: Role of Oxytocin, Prostaglandin F<sub>2</sub> <sub>α</sub>, Progesterone and Noradrenaline

Luteotropic Mechanisms in the Bovine Corpus Luteum: Role of Oxytocin, Prostaglandin F<sub>2</sub> <sub>α</sub>, Progesterone and Noradrenaline

Macrophages Are the Major Source of Tumor Necrosis Factor α in the Porcine Corpus Luteum1

Oxytocin Receptor Regulation and Action in a Human Granulosa-Lutein Cell Line1

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Luteotropic and Luteolytic Effects of Peptides in the Porcine and Human Corpus Luteum

Cited by 7 publications

References 17 publications

Luteotropic Mechanisms in the Bovine Corpus Luteum: Role of Oxytocin, Prostaglandin F<sub>2</sub> <sub>&alpha;</sub>, Progesterone and Noradrenaline

Luteotropic Mechanisms in the Bovine Corpus Luteum: Role of Oxytocin, Prostaglandin F<sub>2</sub> <sub>&alpha;</sub>, Progesterone and Noradrenaline

Macrophages Are the Major Source of Tumor Necrosis Factor α in the Porcine Corpus Luteum1

Oxytocin Receptor Regulation and Action in a Human Granulosa-Lutein Cell Line1

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Product

Resources

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Luteotropic Mechanisms in the Bovine Corpus Luteum: Role of Oxytocin, Prostaglandin F<sub>2</sub> <sub>α</sub>, Progesterone and Noradrenaline

Luteotropic Mechanisms in the Bovine Corpus Luteum: Role of Oxytocin, Prostaglandin F<sub>2</sub> <sub>α</sub>, Progesterone and Noradrenaline