Lycium barbarum Polysaccharides Protect Human Lens Epithelial Cells against Oxidative Stress–Induced Apoptosis and Senescence

Abstract

ObjectivesWe aimed to investigate the protective effect of Lycium barbarum polysaccharides (LBPs) against oxidative stress–induced apoptosis and senescence in human lens epithelial cells.MethodsTo study apoptosis, SRA01/04 cells, a human lens epithelial cell lines, were exposed to 200 µM hydrogen peroxide (H2O2) for 24 h with or without pretreatment with LBPs. Cell viability was measured using a Cell Counting Kit-8 (CCK-8) assay. Cell apoptosis, intracellular reactive oxygen species (ROS), and the loss of mitochondria membrane potential (Δψm) were detected by flow cytometric analyses. Expression levels of Bcl-2 and Bax proteins were measured by western blot analysis. The levels of malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione (GSH) were quantized using commercial enzymatic kits according to the manufacturer's instructions. To study senescence, SRA01/04 cells were pre-incubated with LBPs and all cells were then exposed to 100 µM H2O2 for 96 h. Cellular senescence was assessed by morphologic examination and senescence-associated β-galactosidase (SA-β-gal) staining.ResultsLBPs significantly reduced H2O2-induced cell apoptosis, the generation of ROS, the loss of Δψm, and the levels of MDA. LBPs also inhibited H2O2-induced downregulated Bcl-2 and upregulated Bax proteins and increased the levels of SOD and GSH enzyme activity. Moreover, LBPs significantly attenuated H2O2-induced cellular senescence.ConclusionsThese findings suggested that LBPs protect human lens epithelial cells from H2O2-induced apoptosis by modulating the generation of ROS, loss of Δψm, Bcl-2 family, and antioxidant enzyme activity and attenuating cellular senescence.

Lycium Barbarum Polysaccharides Alleviates Oxidative Damage Induced by H2O2 Through Down-Regulating MicroRNA-194 in PC-12 and SH-SY5Y Cells

“…Meanwhile, alterations of those proteins were mitigated by LBPs, which illustrated LBPs reduced apoptosis through repressing the intrinsic apoptosis pathway in PC-12 cells. The anti-apoptotic role of LBPs in H 2 O 2 -induced PC-12 cells was coincident with that in human lens epithelial cells [32].…”

Lycium barbarum polysaccharides promoted proliferation and differentiation in osteoblasts

“…12 A bunch of studies has shown that LBP has various bioactivities as well as antioxidant characteristics. LBP is also considered to have protective effects on lens epithelial cells 18 and myocardial cells. This effect is likely to be correlated with its antioxidant effect and we hypothesized that LBP treatment could protect the osteoblast from the stress.…”

Mir-22-3p Enhances the Chemosensitivity of Gastrointestinal Stromal Tumor Cell Lines to Cisplatin through PTEN/PI3K/Akt Pathway

“…After 5 h incubation at 37°C, Cis and Mir + Cis cells were exposed to 10 μM cisplatin for 24h, other cells did not expose to cisplatin. 14 Then the medium was removed and added 100 μL fresh medium containing 10% CCK-8 (Dojindo, Kumamoto, Japan) to each well. The plate was incubated for 1-4 hours, and the absorbance at 450 nm was determined using a microplate reader (Thermo Fisher Scientific, Waltham, MA, USA).…”