Lycopene increases the proportion of slow-twitch muscle fiber by AMPK signaling to improve muscle anti-fatigue ability

Wen, Wanxue; Chen, Xiaoling; Huang, Zhiqing; Chen, Shuai; Yu, Bing; He, Jun; Zheng, Ping; Luo, Yuheng; Yan, Hui; Yu, Jie

doi:10.1016/j.jnutbio.2021.108750

Cited by 19 publications

(19 citation statements)

References 38 publications

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“…Some studies have suggested that PGC-1a stimulates fiber-type conversion in muscles. 5,[39][40][41] However, in our study, the expression levels of MyHC I and PGC-1⊍ were inconsistent. This may be because an increase in PGC-1⊍ expression is not the only factor that promotes muscle fiber conversion.…”

Section: Discussioncontrasting

confidence: 61%

Balanced branched‐chain amino acids modulate meat quality by adjusting muscle fiber type conversion and intramuscular fat deposition in finishing pigs

Zhang

Guo

et al. 2022

J Sci Food Agric

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BACKGROUND Pork is an important food for humans and improving the quality of pork is closely related to human health. This study was designed to investigate the effects of balanced branched‐chain amino acid (BCAA)‐supplemented protein‐restricted diets on meat quality, muscle fiber types, and intramuscular fat (IMF) in finishing pigs. RESULTS The results showed that, compared with the normal protein diet (160 g kg−1 crude protein), the reduced‐protein diet (120 g kg−1 crude protein) supplemented with BCAAs to the ratio of 2:1:2 not only had higher average daily gain (P < 0.05) and carcass weight (P < 0.05) but also improved meat tenderness and juiciness by decreasing shear force (P < 0.05) and increasing water‐holding capacity (P < 0.05). In particular, this treatment showed higher (P < 0.05) levels of phospho‐acetyl‐CoA carboxylase (P‐ACC) and peroxisome proliferation‐activated receptor‐γ (PPARγ), and lower (P < 0.05) levels of P‐adenosine 5‘‐monophosphate (AMP)‐activated protein kinase (P‐AMPK), increasing the composition of IMF and MyHC I (P < 0.05) in the longissimus dorsi muscle (LDM). In terms of health, this group increased eicosapentaenoic acid (EPA) (P < 0.01) and desirable hypocholesterolemic fatty acids (DHFA) (P < 0.05), and decreased atherogenicity (AI) (P < 0.01) and hypercholesterolemic saturated fatty acids (HSFA) (P < 0.05). CONCLUSION Our findings suggest a novel role for a balanced BCAA‐supplemented restricted protein (RP) diet in the epigenetic regulation of more tender and healthier pork by increasing IMF deposition and fiber type conversion, providing a cross‐regulatory molecular basis for revealing the nutritional regulation network of meat quality. © 2021 Society of Chemical Industry.

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Section: Discussioncontrasting

confidence: 61%

Balanced branched‐chain amino acids modulate meat quality by adjusting muscle fiber type conversion and intramuscular fat deposition in finishing pigs

Zhang

Guo

et al. 2022

J Sci Food Agric

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“…To further explore the direct effect of CLA on skeletal muscle, C2C12 myoblasts were used to study the effect of t10, c12-CLA. A large body of evidence has shown that changes on C2C12 myoblasts can well reflect the changes in skeletal muscle . Our results showed that t10, c12-CLA directly changed muscle fiber type and oxidative metabolism in C2C12 myoblasts.…”

Section: Discussionsupporting

confidence: 56%

trans 10, cis 12, but Not cis 9, trans 11 Conjugated Linoleic Acid Isomer Enhances Exercise Endurance by Increasing Oxidative Skeletal Muscle Fiber Type via Toll-like Receptor 4 Signaling in Mice

Duan

Yin

et al. 2021

J. Agric. Food Chem.

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Conjugated linoleic acid (CLA) has been implicated in regulating muscle fiber. However, which isomer elicits this effect and the underlying mechanisms remain unclear. Here, male C57BL6/J mice and C2C12 cells were treated with two CLA isomers, and the exercise endurance, skeletal muscle fiber type, and involvement of Toll-like receptor 4 (TLR4) signaling were assessed. The results demonstrated that dietary t10, c12, but not c9, t11-CLA isomer enhanced exercise endurance of mice (from 115.88 ± 11.21 to 130.00 ± 15.84 min, P < 0.05) and promoted the formation of oxidative muscle fiber type of gastrocnemius muscle (from 0.15 ± 0.04 to 0.24 ± 0.05, P < 0.05). Consistently, t10, c12-CLA isomer increased the mRNA expression of oxidative muscle fiber type in C2C12 myotubes (from 1.00 ± 0.08 to 2.65 ± 1.77, P < 0.05). In addition, t10, c12-CLA isomer increased TLR4 signaling expression in skeletal muscle and C2C12 myotubes. More importantly, knockdown of TLR4 eliminated the t10, c12-CLA isomer-induced enhancement of exercise endurance in mice and elevation of oxidative muscle fiber type in C2C12 myotubes and gastrocnemius muscle. Together, these findings showed that t10, c12, but not c9, t11-CLA isomer enhances exercise endurance by increasing oxidative skeletal muscle fiber type via TLR4 signaling.

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“…Through mechanism studies, it was found that citrus tangeretin increased the phosphorylation of LKB1 kinase Ser428 through energy regulation, and then activated the mitochondrial biogenesis AMPK-PGC1α-NRF1-TFAM signaling pathway, which played an active role in regulating mitochondrial biogenesis and delayed the occurrence of fatigue ( 60 ). Lycopene activates the protein expression of skeletal muscle metabolic regulators (AMPK, NRF1, and PGC-1α) and promotes mitochondrial biosynthesis, thereby enhancing exercise endurance ( 61 ). These results suggest that LCBP can improve physical performance, and the occurrence of antagonistic fatigue is closely related to the activation of the skeletal muscle AMPK-PGC1α-NRF1-TFAM signal axis and the enhancement of mitochondrial biogenesis in skeletal muscle.…”

Section: Discussionmentioning

confidence: 99%

Lonicera caerulea Berry Polyphenols Extract Alleviates Exercise Fatigue in Mice by Reducing Oxidative Stress, Inflammation, Skeletal Muscle Cell Apoptosis, and by Increasing Cell Proliferation

et al. 2022

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Exercise fatigue can exert deleterious effects on the body. This study evaluated the effects and mechanisms by which Lonicera caerulea berry polyphenols extract (LCBP) improved the treadmill endurance of mice. Comparison was performed between the effects at 25°C and low temperatures (-5°C). Energy storage, product metabolism, and other biochemical indices were determined using vitamin C (VC) as a positive control. Co-immunoprecipitation was performed to detect the interaction between different proteins. Dietary supplementation with LCBP significantly prolonged the exhaustion time during treadmill exercise by 20.4% (25 °C) and 27.4% (-5 °C). LCBP significantly regulated the expression of antioxidant and inflammatory proteins, Bcl-2 /Bax apoptosis proteins, and the PKCα -NOx2 / Nox4 pathway proteins, and activated the expression of AMPK-PGC1α -NRF1-TFAM proteins in skeletal muscle mitochondria. The gene and protein expression of miRNA-133a/IGF-1/PI3K/Akt/mTOR in skeletal muscle cells was also activated. Molecular docking confirmed that the main components of LCBP such as cyanidin-3-glucoside, catechin, and chlorogenic acid, have strong binding affinity toward AMPKα. LCBP alleviates exercise fatigue in mice by reducing oxidative stress, inflammation, and apoptosis of skeletal muscle cells, enhances mitochondrial biosynthesis and cell proliferation, reduces fatigue, and enhances performance. These effects are also significant in a low-temperature environment (Graphical Abstract). Consequently, these results provide novel insights into the anti- fatigue roles of LCBP in exercise fatigue.

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Lycopene increases the proportion of slow-twitch muscle fiber by AMPK signaling to improve muscle anti-fatigue ability

Cited by 19 publications

References 38 publications

Balanced branched‐chain amino acids modulate meat quality by adjusting muscle fiber type conversion and intramuscular fat deposition in finishing pigs

Balanced branched‐chain amino acids modulate meat quality by adjusting muscle fiber type conversion and intramuscular fat deposition in finishing pigs

trans 10, cis 12, but Not cis 9, trans 11 Conjugated Linoleic Acid Isomer Enhances Exercise Endurance by Increasing Oxidative Skeletal Muscle Fiber Type via Toll-like Receptor 4 Signaling in Mice

Lonicera caerulea Berry Polyphenols Extract Alleviates Exercise Fatigue in Mice by Reducing Oxidative Stress, Inflammation, Skeletal Muscle Cell Apoptosis, and by Increasing Cell Proliferation

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