Lymph vessels visualization from optical coherence tomography data using depth‐resolved attenuation coefficient calculation

Moiseev, Alexander A.; Sirotkina, Marina A.; Potapov, Arseniy L.; Matveev, Lev A.; Vagapova, Nailya N.; Kuznetsova, Irina A.; Gladkova, Natalia D.

doi:10.1002/jbio.202100055

Cited by 8 publications

(8 citation statements)

References 44 publications

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“…OCT angiography is based on analysis of speckle structure [10,11]. OCT lymphangiography is based on analysis of the three-dimensional distribution of the attenuation coefficient with depth resolution [12]. 3D OCT data were obtained from the inner surface of the labia minora, which was then marked with methylene blue for targeted biopsy.…”

Section: Multimodal Optical Coherence Tomographymentioning

confidence: 99%

Noninvasive assessment of morphological and vascular features of the normal vulvar skin using multimodal OCT

Potapov¹,

Bychkova

Saidova

et al. 2023

Photonics in Dermatology and Plastic Surgery 2023

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The vulva is a specific topographic region of the skin with complex morphology and a variety of functions. The combination of the urinary, reproductive and gastrointestinal systems in the same area makes the vulva susceptible to a wide range of diseases that require a multidisciplinary approach. As a result of these anatomical and physiological features, vulvar diseases are difficult to diagnose and insufficiently recognized. Multimodal optical coherence tomography (MM OCT) is an optical tool that allows real-time assessment of the structure of vulvar tissues with a resolution of 10-15 μm, and also provides label-free visualization of blood and lymphatic vessels. The advantages of the MM OCT method are its non-invasiveness, the possibility of examining a large tissue area, as well as obtaining data from various parts of the organ under study. In this paper, we use cross-polarization OCT, angiography, and lymphangiography with parallel histological examination to describe the structure of normal hairless vulvar skin. Two variants of the structure of vulvar skin were identified: skin with epidermal ridges, skin without epidermal ridges. For the first time, a change in the architecture of the microvasculature in response to a change in the structure of the epidermis has been described. Knowledge and understanding of the normal processes of change in the skin of the vulva are the main study of any pathology that occurs in this organ.

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Section: Multimodal Optical Coherence Tomographymentioning

confidence: 99%

Noninvasive assessment of morphological and vascular features of the normal vulvar skin using multimodal OCT

Potapov¹,

Bychkova

Saidova

et al. 2023

Photonics in Dermatology and Plastic Surgery 2023

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“…Optical coherence tomography (OCT) is a non-invasive, contrast-agent-free, volumetric imaging technique that has already shown promise toward in-vivo lymphography, 8 – 10 and neurography 11 , 12 . Owing to the aforementioned transparency of these structures, most OCT approaches rely on negative-contrast intensity-thresholding 9 , 13 – 15 for detection, 8 , 9 whereby the absence of a signal in a region surrounded by an otherwise signal-rich region indicates a lymphatic vessel. However, as the signal-noise-ratio (SNR) drops with increasing imaging depth, it becomes progressively harder to differentiate absence-of-signal lymphatics from background noise.…”

Section: Introductionmentioning

confidence: 99%

“…9,14,15 Additionally, lymphatics have been detected utilizing vesselness models based on Hessian filters applied to B-scans, albeit with limited resolution and imaging depth challenges similar to negative-contrast methods. 15,16 Lastly, another method applies depth-resolved attenuation coefficient distributions to visualize lymphatic networks with improved contrast and resolution when compared to intensity thresholding and Hessian filtering techniques, 6,13,17 but the chances of falsepositive lymphatic detections are higher. Utilizing OCT for non-invasive nerve visualization is a field yet to be thoroughly investigated, although there have been promising initial feasibility reports on OCT peripheral nerve identification.…”

Section: Introductionmentioning

confidence: 99%

Detection and differentiation of semi-transparent materials simulating biological structures using optical coherence tomography: a phantom study

et al. 2022

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Significance: Lymphatic and peripheral nervous system imaging is of prime importance for monitoring various important pathologic processes including cancer development and metastasis, and response to therapy.Aim: Optical coherence tomography (OCT) is a promising approach for this imaging task but is challenged by the near-transparent nature of these structures. Our aim is to detect and differentiate semi-transparent materials using OCT texture analysis, toward label-free neurography and lymphography. Approach:We have recently demonstrated an innovative OCT texture analysis-based approach that used speckle statistics to image lymphatics and nerves in-vivo that does not rely on negative contrast. However, these two near-transparent structures could not be easily differentiated from each other in the texture analysis parameter space. Here, we perform a rigorous follow-up study to improve upon this differentiation in controlled phantoms mimicking the optical properties of these tissues. Results:The results of the three-parameter Rayleigh distribution fit to the OCT images of six types of tissue-mimicking materials varying in transparency and biophysical properties demonstrate clear differences between them, suggesting routes for improved lymphatics-nerves differentiation. Conclusions:We demonstrate a novel OCT texture analysis-based lymphatics-nerves differentiation methodology in tissue-simulating phantoms. Future work will focus on longitudinal in-vivo lymphangiography and neurography in response to cancer therapeutics toward adaptive personalized medicine.

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“…The purpose of this study is the description of polymorphic changes in connective tissue of VLS at different lesion degrees using MPM. Our research group plans to use the efforts we have made to isolate the degrees of dermal remodeling in VLS in the future for the development of an intravital high-resolution imaging method-multimodal optical coherence tomography [35][36][37] and its use in the diagnosis and monitoring of VLS treatment.…”

mentioning

confidence: 99%

Multiphoton microscopy assessment of the structure and variability changes of dermal connective tissue in vulvar lichen sclerosus: A pilot study

Potapov

Sirotkina

Matveev

et al. 2022

Journal of Biophotonics

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In this article, we offer a novel classification of progressive changes in the connective tissue of dermis in vulvar lichen sclerosus (VLS) relying on quantitative assessment of the second harmonic generation (SHG) signal received from formalin fixed and deparaffinized tissue sections. We formulate criteria for distinguishing four degrees of VLS development: Initial‐Mild‐Moderate‐Severe. Five quantitative characteristics (length and thickness type I Collagen fibers, Mean SHG signal intensity, Skewness and Coherence SHG signal) are used to describe the sequential degradation of connective tissue (changes in the structure, orientation, shape and density of collagen fibers) up to the formation of specific homogeneous masses. Each of the degrees has a characteristic set of quantitatively expressed features. We focus on the identification and description of early, initial changes of the dermis as the least specific. The results obtained by us and the proposed classification of the degrees of the disease can be used to objectify the dynamics of tissue changes during treatment.

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Lymph vessels visualization from optical coherence tomography data using depth‐resolved attenuation coefficient calculation

Cited by 8 publications

References 44 publications

Noninvasive assessment of morphological and vascular features of the normal vulvar skin using multimodal OCT

Noninvasive assessment of morphological and vascular features of the normal vulvar skin using multimodal OCT

Detection and differentiation of semi-transparent materials simulating biological structures using optical coherence tomography: a phantom study

Multiphoton microscopy assessment of the structure and variability changes of dermal connective tissue in vulvar lichen sclerosus: A pilot study

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