Lymphatic absorption of choline plasmalogen is much higher than that of ethanolamine plasmalogen in rats

Abstract

Lymphatic absorption of PlsCho is much higher than that of PlsEtn in rats, and plasmalogens are re-esterified as 20:4-rich forms in the small intestine.

Serum choline plasmalogens, particularly those with oleic acid in sn-2, are associated with proatherogenic state

“…Previously, we performed an advanced comprehensive analysis of serum ether glycerophospholipids, including Pls, by LC/MS/MS, which has allowed the accurate quantifi cation of each molecular species ( 16,17 ). The present study provides the reference data from 428 Japanese asymptomatic subjects on their serum levels of individual ether glycerophospholipid species.…”

“…LC/MS/MS was conducted following a slightly modifi ed method described previously ( 16 ). LC separation was performed using an Accela UPLC system (Thermo Fisher Scientifi c, Inc., Waltham, MA) with a BEH C8 column (1.7 m, 100 mm × 2.1 mm; Waters Corp., Milford, MA) at 60°C and a flow rate of 450 l/min.…”

Plasma/Serum Plasmalogens

“…In fact, we established the analytical method for PlsCho molecular species with UPLC-MS/MS [38]. MeOH containing 1,2-dimyristoyl-phosphatidylcholine (DMPC) and phosphatidylethanolamine (DMPE) as internal standard (IS) and dibutyl hydroxytoluene (BHT) as antioxidant was added to the freeze-dried material and shaken (30 s).…”

The Proportion of Nervonic Acid in Serum Lipids is Associated with Serum Plasmalogen Levels and Metabolic Syndrome

“…Liquid chromatography-tandem mass spectrometry was conducted following a slightly modified method described previously 26 . Liquid chromatography separation was performed using an Accela UPLC system Thermo Fisher Scientific Inc., Waltham, MA, USA with a BEH C8 column 1.7 μm, 100 mm 2.1 mm i.d.…”