1974
DOI: 10.1084/jem.139.3.732
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Lymphocyte Proliferation in Vitro Induced by Hapten Autologous Protein Conjugates

Abstract: Lymphocyte proliferation provides an in vitro model of cellular immunity. Thus, in guinea pigs the time-course and the specificity characteristics of this in vitro reaction mimics the delayed type hypersensitivity (DTH) 1 reaction in vivo (1-3). Both responses are characterized by a high degree of specificity, e.g., in hapten-carrier systems DTH reactions are elicited most readily by the immunizing hapten-protein conjugate (4-6). If elicitation of a DTH reaction is attempted with the hapten conjugated to a pro… Show more

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Cited by 16 publications
(5 citation statements)
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“…These characteristics did not change as a function of time after in vivo immunization. Previously, we have shown that there was no change in the antigen dose requirement as a function of time after in vivo immunization for the in vitro proliferating T cell subset [26].…”
Section: Can Th Cell Activity Be Mediated Via Membrane Antigens Othermentioning
confidence: 90%
See 1 more Smart Citation
“…These characteristics did not change as a function of time after in vivo immunization. Previously, we have shown that there was no change in the antigen dose requirement as a function of time after in vivo immunization for the in vitro proliferating T cell subset [26].…”
Section: Can Th Cell Activity Be Mediated Via Membrane Antigens Othermentioning
confidence: 90%
“…Addition of exogenous IL 2 to cultures of TNP-LPS-primed B/M@ cells, TNP-conjugated mAb and the Th cell clones did not induce an augmented TNP-specific PFC response above that induced by IL2 alone (not shown). TcR is involved in this process [4,5,12,[22][23][24][25][26]. As a consequence of TcR triggering and Th-B cell contact, a number of nonspecific helper factors are produced.…”
Section: Can Th Cell Activity Be Mediated Via Membrane Antigens Othermentioning
confidence: 99%
“…We have shown that Tr cells with specificity for protein antigens like ovalbumin or human serum albumin could be retained on immunoglobulin (Ig) anti‐Ig bead columns as well as on antigen‐coated bead columns [3]. These data implied that protein‐specific Tr cells may have Fc receptors (or passively absorbed Ig) on their surface membrane, and that they did not behave like Th cells or CTL, which could not be absorbed on either type of immunoadsorbent [23]. Although the regulation in our hapten‐carrier system was dependent on Thy‐1‐positive cells, further attempts to characterize these cells failed because of our inability to detach the cells from the immunoadsorbent and to expand them either in vivo or in vitro .…”
Section: Antigen‐specific Tr Cellsmentioning
confidence: 99%
“…In contrast, the Vβ8.2Jβ2.7‐specific T‐cell lines proliferated specifically in the presence of the B3, B5 and p41–50 peptides, indicating that these peptides may associate with IA u and interact with CD4 + T cells. Such T cells may be implicated in the protection against EAE by some unknown mechanism [4–8, 23–28, 41], or they may serve as helpers for the CD8 + T cells with Vβ8.2 peptide specificity [41].…”
Section: Tr Cell Inductionmentioning
confidence: 99%
“…In parallel with in vivo results on the minor importance of macrophages in secondary responses [ 5-61 we found that the secondary DNA synthetic response of guinea pig lymph node cells in vitro was the same before or after the lymphocytes had been passed through Ig anti-Ig-coated columns. Such columns very efficiently deplete a lymphocyte population of Ig-positive cells, F c receptor-bearing cells and adherent cells [ 1, data are at variance with those of Rosenthal et al who reported that macrophages play an obligatory role in the antigen presentation to the T cells in vitro [ 10-161: Another feature of the secondary in vivo response is that it can be elicited by very small amounts of antigen even in the presence of rather high levels of persisting primary antibodies [17][18]. Therefore, we reinvestigated the importance of adherent cells, Fc receptor-bearing cells and membrane Ig-positive cells in our lymphoproliferative assay using more functional and morphological criteria to characterize these cells.…”
Section: Introductionmentioning
confidence: 99%