LYMPHOCYTE PROLIFERATION ON HYPERSENSITIVITY OF Balb/C MICE AFTER GIVEN ETHANOL EXTRACT TUBER OF Dioscorea alata L.

Abstract

Purple yam (Dioscorea alata L.) is a source nutritious tubers that has not been used optimally. Nutrients content in Dioscorea species are starch, essentials amino acid, minerals, polyphenol, glycoprotein, purin derivates such as allantoin, and steroid saponin which have biological activity such as immunomodulatory and antiallergic. The purpose of this research was to assess the absolute number of lymphocyte on hypersensitivity of mice after treated with ethanol extract of D. alata. Twenty one male Balb/C mice were used which were divided into seven groups: control (C), negative control (NC), treatments with ethanol extract of D. alata 0.17 g/kg, 2.01 g/kg, 10.04 g/kg (T I -T III), treatment with antihistamine drug 0,4 mg/mice/day (T IV) and treatment with diosgenin 200 mg/kg (T V). For 17 consecutive days the T I -T III groups were treated with ethanol extract of D. alata correspond to their doses, T IV group were treated with antihistamine drugs, and T V group were treated with diosgenin. On day 15, NC and T I -T V groups of mice were induced by ovalbumin 0,0483 mg/mice. Mice were sacrificed on day 18, and the lymphocyte was isolated from spleen, and the absolute number of lymphocyte was counted with Haemocytometer. Results showed that the absolute number of lymphocyte on mice hypersensitivity after treated with ethanol extract of D. alata L. were the lowest, while the highest absolute number of lymphocyte was found in the group treated with antihistamine drugs followed by the group treated with diosgenin on 200 mg/kg BW, negative control group and control group, respectively. Keywords: Balb/C mice, Dioscorea alata, ovalbumin, hypersensitivity, lymphocyte INTRODUCTIONPurple yam (Dioscorea alata L.) is a source of nutritious tubers that has not been used optimally. Nutrients content in Dioscorea species are starch, essentials amino Acid, mineral, polyphenol, mucilage (glycoprotein), purin derivates (such as allantoin) and steroid saponin (Wanasundera & Ravindran, 1994;Lape & Treche, 1994; Agbor-Egbe & Treche, 1995;Hikino et al.,1986, He et al., 1994Hu et al., 1996;Hu et al.,1997;Yang et al., 2003;Wang et al., 2011;Yoon et al., 2008). D. alata L. contains diosgenin (Cheng et al., 2007), a main steroidal saponin steroid aglicon as intermediate steroidal in pharmaceutical manufacture. Steroidal saponin is the most important bioactive compound due to its biological functions such as anticarcinogenic, antithrombotic, antiviral, hemolitic, hypocholesterolemic, hypoglichemic, immunostimulatory, antitumorogenic, antimutagenic, immunomodulatory and antiinflammatory (He et al., 1994;Hu et al., 1996;Hu et al., 1997;Yang et al.,2003). Results of previous studies showed that steroidal saponin has antiallergic activity (Zhang et al., 2012). The purpose of the research was to assess the absolute number of lymphocyte on hypersensitivity of mice after treated with ethanol extract of Dioscorea alata L. tubers.

Steroidal Saponin Isolated from Dioscorea alata L. Extract Improve Digestive Tract Allergy in Balb/C Mice Induced by Ovalbumin

“…However, it has not been much utilized optimally (Christina & Rifa'i, 2014). Our previous research proved that an ethanol extract of D. alata had hypo-allergenic and immuno-modulatory activity (Makiyah, 2018;Makiyah et al, 2014Makiyah et al, , 2016Makiyah et al, , 2015. Steroidal saponin is the most crucial compounds contained in Dioscorea species because it has some biological functions such as immunomodulatory and antiinflammatory depend on its structure (Dong et al, 2008;Kaur et al, 2021;Wang et al, 2011).…”

In Silico Toxicity Prediction of Bioactive Compounds of Dioscorea alata L.

“…In addition, research on D. alata L. is concerned with the activity of scavenging reactive oxygen species, antioxidants, antidiabetics, anticlastogenic, antiosteoporotic, hypoallergenic, and immunomodulatory activities. [1][2][3][4][5][6][7] However, D. alata L. is used as food and traditional medicine empirically from generation to generation. Based on the development of knowledge and research, it is necessary to know the safety of traditional medicine to avoid unwanted harmful or toxic effects, such as toxicity effect.…”