Lymphocyte subpopulations in high endothelial venules and lymphatic capillaries in rat Peyer's patches: An immunoelectron microscopic study.

Otsuki, Yoshinori; Magari, Sumiko

doi:10.1267/ahc.21.33

Cited by 7 publications

(18 citation statements)

References 12 publications

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“…The percentage of lymphocyte subsets was characteristic in MALT. The major lymphocyte subsets were IgG-and IgM-positive cells in mouse mediastinal milky spots, T cells, IgA-and IgM-positive cells in rat GALT (OTSUKI et al, 1988), pan T cells in rat BALT (OTSUKI et al, 1989a), and suppressor T cells in human fallopian tube-and uterus-associated lymphoid tissues (MORRIS et al, 1986;OTSUKI et al, 1989b;OTSUKI et al, 1990). It remains to be determined if the variety of major lymphocyte subsets among these lymphoid tissues is caused by different exogenous antigens coming to the surface of the lymphoid tissues, or by organ-specificity or species difference.…”

Section: Discussionmentioning

confidence: 99%

“…Gut-associated lymphoid tissues (GALT) and Table 1. Percentages of cells (mean+standard deviation) labeled with various antibodies in the mediastinal milky spots of mice bronchus-associated lymphoid tissues (BALT) are always exposed to the exogenous antigens through the epithelium, and the structure of these tissues are in some respects similar to that of lymph nodes: the presence of a germinal center, HEV in the T cell area and lymphatic capillaries around the lymphoid tissues (SMINIA and PLESCH, 1982;BREEL et al, 1988;OTSUKI et al, 1988OTSUKI et al, ,1989a. On the other hand, mediastinal milky spots as well as fallopian tube- (OTSUKi et al, 1989b) and uterus-associated lymphoid tissues (OTSUKI et al, 1990) are normally under aseptic conditions and possess common structures such as the absence of a germinal center, HEV and lymphatic capillaries, the presence of only a few plasma cells and many lymphocytes and macrophages migrating between epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

“…These recirculating cells are considered to pass between mesothelial cells into the pleural cavity, then enter lymphatic capillaries via macula cribriformlike structures which are located in the intercostal spaces (NAKAI, 1963). However, further studies are needed to delineate the afferent migratory route of lymphocytes and macrophages into the mediastinal milky spots, because mediastinal milky spots possess no HEV or lymphatic capillaries as did the migratory route observed in GALT (OTSUKI et al, 1988) and BALT (OTSUKI et al, 1989a). It is also rare to encounter monocytes and lymphocytes passing through the blood capillaries within the lymphoid tissues.…”

Section: Discussionmentioning

confidence: 99%

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Lymphocyte Subpopulations in Mediastinal Milky Spots of Mice: Light- and Electron-Microscopic Immunohistochemical Observations.

Inoue

Otsuki

1992

Archives of Histology and Cytology

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Summary. The structure and distribution of cellular components in the mediastinal milky spots of C57BL/6 mice were studied by electron microscopy and immunohistochemistry. The mediastinal milky spots were generally covered with plump mesothelial cells with hemidesmosome-like structures in small projections of the cytoplasm, and consisted mainly of clusters of lymphocytes, macrophages and fibroblasts. Macrophages and lymphocytes were often seen passing between mesothelial cells and migrating into the pleural cavity. Neither high endothelial venules (HEY) nor lymphatics were present in the mediastinal milky spots. Thy-1(CD5)-, L3/T4(CD4)-and Lyt-2(CD8)-positive cells were located predominantly at the basal area adjacent to fat cells under the lymphoid tissue. IgG-and Ia-positive cells dominated throughout the lymphoid tissue, whereas IgM-positive cells were observed in the lymphoid tissue other than the basal area. Mediastinal milky spots showed a specific distribution of lymphocyte subsets: the T cell area comprised the basal area, and the B cell area was mainly other areas.The mean percentages of CD4-, CD8-, IgM-, IgG-and IgA-positive cells in the mediastinal milky spots were 16.5%, 20.1%, 28.8%, 37.3% and 2.1%, respectively; the percentage of B cells was higher than that of T cells. IgG-and IgM-positive cells were the main lymphocyte subsets in the mediastinal milky spots. The most characteristic finding was that IgA-positive cells were a very minor cellular component.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Lymphocyte Subpopulations in Mediastinal Milky Spots of Mice: Light- and Electron-Microscopic Immunohistochemical Observations.

Inoue

Otsuki

1992

Archives of Histology and Cytology

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“…However, with the light microscope, lymphatics are often mistaken for artifactual splits in the tissue. We have already reported that the specific characteristics of lymphatics that distinguish them from blood vessels are revealed only by transmission electron microscopy (Otsuki et al, 1986). Our light and electron microscopic observations show that in the fallopian tube of the rabbit lymphatic capillaries arise in the lamina propria mucosa or the periphery of follicles with sparse distribution, run through the muscular layer, and form a dense network in the subserosa.…”

Section: Discussionmentioning

confidence: 66%

“…The details of the two-stage indirect immunoperoxidase procedure are described elsewhere (Otsuki and Magari, 1988). Briefly, fixed materials were immersed in 0.1 M phosphate buffer (PB) for 3 hr and cut into serial sections 40-60 pm thick on a Dosaka microslicer at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Lymphatics and lymphoid tissue of the fallopian tube: Immunoelectronmicroscopic study

et al. 1989

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Lymphoid tissue of the human fallopian tube consists of follicles, lymphoepithelium, and lymphatic and blood capillaries and is located consistently in the interstitial part of the human fallopian tube. Using an immunoelectronmicroscopic technique, we have elucidated the ultrastructure of the lymphoid tissue of the human fallopian tube and the fine distribution and ultrastructure of the lymphatics associated with the rabbit fallopian tube. Lymphatic capillaries arise in the lamina propria mucosa and the periphery of follicles, where they are sparsely distributed, run through the muscular layer, and form a dense network in the subserosa. Characteristic features of the ultrastructure are aggregations of smooth muscle cells, alternating areas of densely and sparsely distributed collagen fibers, and unmyelinated nerve fibers beneath the lymphatic endothelium. Immunoelectronmicroscopic analysis has demonstrated an obvious difference in the distribution of T- and B-lymphocytes in the lymphoid tissue of the human fallopian tube. Many T-lymphocytes are present in the follicles and epithelium, but B-lymphocytes are either absent or rarely found. T-lymphocytes sometimes infiltrate into the basal lamina of the epithelium lying in close contact with the follicles. We conclude that the lymphoid tissue is constantly located in the interstitial part of the human fallopian tube and that intraepithelial lymphocytes, mainly T-lymphocytes, migrate via the basal lamina of the epithelium from follicles. Lymphatic capillaries in the fallopian tube may be the main migratory route of intraepithelial lymphocytes. The intraepithelial lymphocytes and epithelial cells of the fallopian tube have attracted considerable interest as a result of immunological studies of the recognition of spermatozoal antigens and the fertilized ovum.(ABSTRACT TRUNCATED AT 250 WORDS)

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Lymphocyte subpopulations in high endothelial venules and lymphatic capillaries of bronchus‐associated lymphoid tissue (BALT) in the rat

Otsuki¹,

Ito²,

Magari³

1989

Am. J. Anat.

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The subpopulations of lymphocytes and non-lymphoid cells in high endothelial venules (HEV) and in lymphatic capillaries surrounding lymphoid follicles in bronchus-associated lymphoid tissue (BALT) were examined by electron microscopy after preembedding the tissue and staining with an immunoperoxidase technique. The results were compared with those obtained in gut-associated lymphoid tissue (GALT) reported previously. Monoclonal mouse-anti-rat T cell, IgG, IgM, IgA, and Ia antisera were used. Plasma cells that were reactive to anti-IgG, anti-IgM, and anti-IgA were detected as cells in which the 3',3'-diaminobenzidine tetrahydroxychloride reaction product was localized in rough endoplasmic reticulum and perinuclear spaces but not on plasma membranes. These plasma cells did not occur in either lymphatic capillaries or HEV in BALT as they did in GALT. Cells with surface Ig (sIg cells), T-cell antigen (T cells), and Ia antigen (Ia cells) were present in BALT. T cells were located predominantly in the follicular area opposite the bronchial epithelium; IgM- and IgG-reactive cells were found in the follicular area adjacent to the bronchial epithelium; and IgA-positive cells were found in the lateral part of the area where the T cells were localized (T-cell area). Ia cells were abundant throughout BALT and in moderate numbers in the epithelium. A striking observation was the presence of "nurse-cell"-like structures in the periphery of BALT. The percentages of T, sIgG, sIgM, and sIgA cells in the HEV were 54.7%, 2.4%, 28.9%, and 27.3%, respectively, and in the lymphatic capillaries, 41.2%, 3.8%, 38.2%, and 21.2%, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

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Lymphocyte subpopulations in high endothelial venules and lymphatic capillaries in rat Peyer's patches: An immunoelectron microscopic study.

Cited by 7 publications

References 12 publications

Lymphocyte Subpopulations in Mediastinal Milky Spots of Mice: Light- and Electron-Microscopic Immunohistochemical Observations.

Lymphocyte Subpopulations in Mediastinal Milky Spots of Mice: Light- and Electron-Microscopic Immunohistochemical Observations.

Lymphatics and lymphoid tissue of the fallopian tube: Immunoelectronmicroscopic study

Lymphocyte subpopulations in high endothelial venules and lymphatic capillaries of bronchus‐associated lymphoid tissue (BALT) in the rat

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