Lymphocyte Subsets Are Associated with Disease Status in Neuromyelitis Optica Spectrum Disorders

Hong, Yun–Chul; Liu, Wei; Wu, Yifan; Zhu, Dezhi; Shen, Xiafeng; Guan, Yangtai

doi:10.1159/000520745

Cited by 4 publications

(4 citation statements)

References 42 publications

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“…Since the release of AQP-4 antibody is mainly related to B cells ( Nie and Blair, 2022 ), research and treatment of NMOSD has mainly focused on targeting B cells. A study by Yang et al (2021) comprehensively assessed changes in peripheral blood lymphocyte subsets in patients with NMOSD. They found significantly higher levels of CD19 + B cells during active NMOSD than in healthy people.…”

Section: Discussionmentioning

confidence: 99%

Predictive role of blood-based indicators in neuromyelitis optica spectrum disorders

et al. 2023

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IntroductionThis study aimed to assess the predictive role of blood markers in neuromyelitis optica spectrum disorders (NMOSD).MethodsData from patients with NMOSD, multiple sclerosis (MS), and healthy individuals were retrospectively collected in a 1:1:1 ratio. The expanded disability status scale (EDSS) score was used to assess the severity of the NMOSD upon admission. Receiver operating characteristic (ROC) curve analysis was used to distinguish NMOSD patients from healthy individuals, and active NMOSD from remitting NMOSD patients. Binary logistic regression analysis was used to evaluate risk factors that could be used to predict disease recurrence. Finally, Wilcoxon signed-rank test or matched-sample t-test was used to analyze the differences between the indicators in the remission and active phases in the same NMOSD patient.ResultsAmong the 54 NMOSD patients, neutrophil count, neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and systemic immune-inflammation index (SII) (platelet × NLR) were significantly higher than those of MS patients and healthy individuals and positively correlated with the EDSS score of NMOSD patients at admission. PLR can be used to simultaneously distinguish between NMOSD patients in the active and remission phase. Eleven (20.4%) of the 54 patients had recurrence within 12 months. We found that monocyte-to-lymphocyte ratio (MLR) (AUC = 0.76, cut-off value = 0.34) could effectively predict NMOSD recurrence. Binary logistic regression analysis showed that a higher MLR at first admission was the only risk factor for recurrence (p = 0.027; OR = 1.173; 95% CI = 1.018–1.351). In patients in the relapsing phase, no significant changes in monocyte and lymphocyte count was observed from the first admission, whereas patients in remission had significantly higher levels than when they were first admitted.ConclusionHigh PLR is a characteristic marker of active NMOSD, while high MLR is a risk factor for disease recurrence. These inexpensive indicators should be widely used in the diagnosis, prognosis, and judgment of treatment efficacy in NMOSD.

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Section: Discussionmentioning

confidence: 99%

Predictive role of blood-based indicators in neuromyelitis optica spectrum disorders

et al. 2023

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“…Similar decreases in NK cell levels are also observed in other autoimmune diseases, such as Systemic sclerosis (SSc), Systemic lupus erythematosus (SLE), and Neuromyelitis optica spectrum disorder (NMOSD). [31][32][33] NK cell immunotherapy has been used for many diseases, including cancer and autoimmune diseases. [33] This nding may provide a new perspective for the treatment of VN.…”

Section: Discussionmentioning

confidence: 99%

“…[31][32][33] NK cell immunotherapy has been used for many diseases, including cancer and autoimmune diseases. [33] This nding may provide a new perspective for the treatment of VN.…”

Section: Discussionmentioning

confidence: 99%

Immune Alterations in Vestibular Neuritis: A Pilot Study on T and B Lymphocyte Profiles and Cytokine Levels

Song,

Ding,

Kohls

et al. 2024

Preprint

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Objectives Vestibular neuritis (VN) etiology remains elusive, with hypotheses suggesting viral infection, non-infectious inflammation, or immune responses. This study aims to assess the T and B lymphocyte subsets and cytokine expressions in the blood of vestibular neuritis patients. This study shed light on its pathogenesis and provided valuable hematological markers for clinical prognosis.Methods A retrospective analysis was conducted on 25 subjects diagnosed with acute vestibular neuritis at the Department of Neurology, Beijing Luhe Hospital, affiliated with Capital Medical University, from January 2022 to June 2023. Within 1–3 days of onset, all patients underwent blood tests and initial Dizziness Handicap Inventory (DHI) scale evaluations. A secondary DHI assessment was performed upon discharge, between days 7–10 post-onset. A group of 25 healthy individuals served as controls.Results Vestibular Neuritis (VN) patients exhibited elevated levels of total B lymphocytes (CD3-CD19+), helper/inducer (CD3 + CD4+) T cells, and the helper/suppressor (CD4+/CD8+) T cell ratio all of which were statistically significant compared to the control group. Conversely, there was a reduction in the levels of total T lymphocytes (CD3 + CD19-), suppressor/cytotoxic T cells (CD3 + CD8+), and natural killer (NK) cells. Elevated Interleukin-6 (IL-6) levels and decreased Interleukin-17A (IL-17A) levels were seen in the VN group compared to controls. There are differences in the levels of CD3 + CD4 + T cells and CD4+/CD8 + T cell ratio among patients in the three groups of mild, moderate, and severe, with the severe group significantly higher than the mild group. Admission levels of CD3 + CD4 + T cells showed a positive relationship with the DHI-Functional (DHI-F) score within 1–3 days. Admission levels of CD3 + CD4 + T cells and CD4+/CD8 + T cell ratio are positively correlated with all DHI scores (including the DHI Total Index and three DHI Subscales) at discharge 7–10 days post-onset.Conclusions Immunological perturbations are implicated in pathogenesis of vestibular neuritis. Evaluation of these immune markers could offer insights into prognostic outcomes for vestibular neuritis patients, leading to development of therapeutic strategies.

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“…Studies have shown that the CD4 + /CD8 + ratio is significantly decreased in patients with some cancers (such as breast cancer, gastric cancer, and lung cancer) ( Wang et al, 2013 ; Dias Rodrigues et al, 2017 ; Liu, 2022 ), HIV ( Castilho et al, 2019 ), and neuromyelitis optica spectrum disorders ( Yang et al, 2021 ), indicating that the immune function of patients may be reduced. The susceptibility of SCI patients to infection is related to immune disability, which is mainly manifested as disordered proportion of T cell subsets.…”

Section: Discussionmentioning

confidence: 99%

Relationship between gut microbiota and lymphocyte subsets in Chinese Han patients with spinal cord injury

Pang¹,

Wang²,

Xiong³

et al. 2022

Front. Microbiol.

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This study is to investigate the changes of lymphocyte subsets and the gut microbiota in Chinese Han patients with spinal cord injury (SCI). We enrolled 23 patients with SCI and 21 healthy controls. Blood and fecal samples were collected. The proportion of lymphocyte subsets was detected by flow cytometry. 16S rDNA sequencing of the V4 region was used to analyze the gut microbiota. The changes of the gut microbiota were analyzed by bioinformatics. Correlation analysis between gut microbiota and lymphocyte subsets was performed. CD4 + cells, CD4 + /CD8 + ratio and CD4 + CD8 + cells in peripheral blood of SCI patients were significantly lower than those of the control group (P < 0.05). There was no significant difference in B cells and CIK cells between the SCI group and the control group. The gut microbiota community diversity index of SCI patients was significantly higher than that of healthy controls. In SCI patients, the relative abundance of Lachnospiraceae (related to lymphocyte subset regulation), Ruminococcaceae (closely related to central nervous system diseases), and Escherichia-Shigella (closely related to intestinal infections) increased significantly, while the butyrate producing bacteria (Fusobacterium) that were beneficial to the gut were dramatically decreased. Correlation analysis showed that the five bacterial genera of SCI patients, including Lachnospiraceae UCG-008, Lachnoclostridium 12, Tyzzerella 3, Eubacterium eligens group, and Rumencocciucg-002, were correlated with T lymphocyte subsets and NK cells. In the SCI group, the flora Prevotella 9, Lachnospiraceae NC2004 group, Veillonella, and Sutterella were positively correlated with B cells. However, Fusobacterium and Akkermansia were negatively correlated with B cells. Moreover, Roseburia and Ruminococcaceae UCG-003 were positively correlated with CIK cells. Our results suggest that the gut microbiota of patients with SCI is associated with lymphocyte subsets. Therefore, it is possible to improve immune dysregulation in SCI patients by modulating gut microbiota, which may serve as a new therapeutic method for SCI.

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Lymphocyte Subsets Are Associated with Disease Status in Neuromyelitis Optica Spectrum Disorders

Cited by 4 publications

References 42 publications

Predictive role of blood-based indicators in neuromyelitis optica spectrum disorders

Predictive role of blood-based indicators in neuromyelitis optica spectrum disorders

Immune Alterations in Vestibular Neuritis: A Pilot Study on T and B Lymphocyte Profiles and Cytokine Levels

Relationship between gut microbiota and lymphocyte subsets in Chinese Han patients with spinal cord injury

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