Lymphocyte traffic and homing in autoimmune thyroid disorders

Marazuela, Mónica

doi:10.1530/eje.0.1400287

Cited by 32 publications

(23 citation statements)

References 45 publications

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“…Therefore, the analysis of peripheral lymphocytes could be the easiest way to study the immunological status of the patients with thyroid autoimmunity. Indeed, most studies have found an activated phenotype in peripheral blood from HT patients, suggesting a generalised immune disregulation in such diseases (30,31). According to this finding, our patients showed a peripheral intra-lymphocyte cytokine pattern that was different from the healthy subjects, suggesting the existence in the peripheral compartment of markers of the autoimmune process.…”

Section: Discussionsupporting

confidence: 54%

“…Cellular immune responses have been studied in circulating (6,12,18) and infiltrating lymphocytes (6 -10) of the patients with thyroid autoimmune disease. Since in the organ-specific diseases the reliable reflection of the autoimmune state lies in the target organ, the analysis of the intra-thyroid lymphocytes is probably the best way to investigate the immunological status in the patients with thyroid autoimmunity (30). Actually, as thyroid fine-needle aspiration is not frequently performed in HT patients, the data available are mostly obtained from surgical tissue that probably does not reflect the situation present in vivo (30).…”

Section: Discussionmentioning

confidence: 99%

“…Since in the organ-specific diseases the reliable reflection of the autoimmune state lies in the target organ, the analysis of the intra-thyroid lymphocytes is probably the best way to investigate the immunological status in the patients with thyroid autoimmunity (30). Actually, as thyroid fine-needle aspiration is not frequently performed in HT patients, the data available are mostly obtained from surgical tissue that probably does not reflect the situation present in vivo (30). Therefore, the analysis of peripheral lymphocytes could be the easiest way to study the immunological status of the patients with thyroid autoimmunity.…”

Section: Discussionmentioning

confidence: 99%

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Type-1 response in peripheral CD4+ and CD8+ T cells from patients with Hashimoto's thyroiditis

Mazziotti

Sorvillo

Naclerio

et al. 2003

European Journal of Endocrinology

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Objective: In this study we performed single-cell analysis of the intracellular cytokine expression in peripheral CD4+ and CD8 + lymphocytes from patients with Hashimoto's thyroiditis (HT) to investigate the type-1 response separately for the two lymphocyte sub-populations. Design and methods: Twenty-nine patients affected by HT and 20 healthy subjects, matched for sex and age, were enrolled. After the analysis of the lymphocyte sub-populations, the intracellular content of interferon-g (IFN-g) in phorbolmyristate acetate (PMA)-stimulated CD4 + and CD8 + lymphocytes was assayed. Moreover, the CD4 + lymphocytes were also evaluated for the intracellular expression of IL-4. Results: No significant differences in CD3 + , CD4 + and CD8 + lymphocytes were found between HT patients and control subjects. However, the HT patients showed higher numbers of CD4 + IFN-g + , CD4+ IL-4 + and CD8 + IFN-g + ðt-test; P # 0:001Þ cells than the control subjects. Analysing the intracellular expression of IFN-g and IL-4 in relation to thyroid function, we found that the euthyroid patients (18 cases) showed more expression of IL-4 in CD4 + lymphocytes than the control subjects, without any significant modification of IFN-g expression in CD4+ and CD8 + lymphocytes. However, the hypothyroid patients (11 cases) showed an increase of IFN-g expression in both CD4 + and CD8 + lymphocytes with respect to the control subjects and the euthyroid patients. Moreover, the expression of IL-4 in CD4 + cells from hypothyroid patients was significantly lower than that seen in the euthyroid cases and comparable to that found in the control subjects. Conclusions: Our study has demonstrated that the peripheral CD4 + and CD8 + T lymphocytes from the HT patients show a type-1 activation strictly correlated to the occurrence of hypothyroidism. Further studies will be needed to clarify the exact role of peripheral lymphocytes in HT and whether they could provide a reliable marker of thyroid immune involvement.

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Type-1 response in peripheral CD4+ and CD8+ T cells from patients with Hashimoto's thyroiditis

Mazziotti

Sorvillo

Naclerio

et al. 2003

European Journal of Endocrinology

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“…We conclude that the alterations in the expression of adhesion molecules on peripheral blood mononuclear cells might suggest their role in the development of GD. GD is an AITD characterized by lymphocytic infiltration of the thyroid gland and the production of TRAb, symporter Na ϩ /I Ϫ , thyroglobulin, and thyroperoxidase antigen (1)(2)(3). In addition, literature data underline the role of cytokines and adhesion molecules as new unspecific markers of the autoimmune process in GD (4 -7).…”

Section: And Cd54mentioning

confidence: 99%

Expression of Very Late Antigen-4 and Lymphocyte Function-Associated Antigen-1 on Peripheral Blood Lymphocytes from Patients with Graves Disease

BOSSOWSKI¹

2002

Pediatric Research

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“…GD is an AITD characterized by lymphocytic infiltration of the thyroid gland and the production of TRAb, symporter Na ϩ /I Ϫ , thyroglobulin, and thyroperoxidase antigen (1)(2)(3). In addition, literature data underline the role of cytokines and adhesion molecules as new unspecific markers of the autoimmune process in GD (4 -7).…”

mentioning

confidence: 99%

Expression of Very Late Antigen-4 and Lymphocyte Function-Associated Antigen-1 on Peripheral Blood Lymphocytes from Patients with Graves Disease

et al. 2002

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The aim of the study was to estimate the expression of ␤1 integrin CD49d (very late antigen-4), ␤2 integrin CD11a (lymphocyte function-associated antigen 1 alpha), L-selectin (CD62L), and intercellular adhesion molecule-1 [ICAM-1 (CD54)] on peripheral blood mononuclear cells in patients with Graves disease (GD) (n ϭ 45, mean age 15.9 Ϯ 5.9 y), in patients with nontoxic nodular goiter (NTNG) (n ϭ 25, mean age 15.2 Ϯ 5.7 y), and in sex-and age-matched healthy control subjects (n ϭ 25, mean age 15.9 Ϯ 2.4 y). The expression of the lymphocyte adhesion molecules was analyzed by the three-color flow cytometry. Decreased percentages of CD49d ϩ and CD11a ϩ lymphocytes were observed in untreated Graves' patients in comparison to healthy controls (p Ͻ 0.007, p Ͻ 0.036) and non-toxic nodular goiter patients (p Ͻ 0.006, p Ͻ 0.019). The analysis of CD62L ϩ and CD54 ϩ antigen expression on peripheral blood lymphocytes revealed increased percentages of L-selectin-and ICAM-1-positive cells in patients with GD (p Ͻ 0.005 for CD62L, p Ͻ 0.008 for ICAM-1) before methimazole therapy, compared with healthy controls. These abnormalities were absent in children with NTNG. In patients with untreated GD, a positive correlation was found between serum levels of free thyroxine and the percentage of CD54 ϩ lymphocytes (r ϭ 0.47, p Ͻ 0.036). GD is an AITD characterized by lymphocytic infiltration of the thyroid gland and the production of TRAb, symporter Na ϩ /I Ϫ , thyroglobulin, and thyroperoxidase antigen (1-3). In addition, literature data underline the role of cytokines and adhesion molecules as new unspecific markers of the autoimmune process in GD (4 -7).Adhesion molecules are the cellular membrane proteins expressed on activated lymphocytes, found to play a pivotal role in the initiation, localization, and perpetuation of thyroid autoimmune disease. They belong to three structurally related families: integrin, Ig supergene, and selectin. Integrins constitute a family of widespread ␣-␤ heterodimeric adhesion receptors that mediate cell attachment to extracellular matrix proteins (fibronectin, laminin, collagen) and cell-cell interactions, which have been grouped in four distinct subfamilies based on ␤-subunit utilization (8

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Lymphocyte traffic and homing in autoimmune thyroid disorders

Cited by 32 publications

References 45 publications

Type-1 response in peripheral CD4+ and CD8+ T cells from patients with Hashimoto's thyroiditis

Type-1 response in peripheral CD4+ and CD8+ T cells from patients with Hashimoto's thyroiditis

Expression of Very Late Antigen-4 and Lymphocyte Function-Associated Antigen-1 on Peripheral Blood Lymphocytes from Patients with Graves Disease

Expression of Very Late Antigen-4 and Lymphocyte Function-Associated Antigen-1 on Peripheral Blood Lymphocytes from Patients with Graves Disease

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