Lymphoepithelial Cyst of the Salivary Gland in a Small Ruminant Lentivirus-Positive Goat

Dolka, I.; Tomaszewski, Marek; Wola, Daria; Czopowicz, Michał; Kaba, Jarosław

doi:10.3390/ani10091545

Cited by 3 publications

(2 citation statements)

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“…CIP is the leading pathology in the course of maedi-visna disease [29] and its severity correlates with peripheral provirus levels [30]. Although progressive arthritis is the main clinical manifestation of CAE, several studies and case reports have shown that interstitial pneumonia also develops in SRLV-infected goats [16][17][18][19][20]31]. Nevertheless, its prevalence in SRLV-infected goats has never been investigated in any large-scale disease survey.…”

Section: Discussionmentioning

confidence: 99%

“…After dewaxing in xylene and rehydration in alcohols, the slides were microwaved in 0.02 M citrate buffer, pH 6.0, at 600 Watt for 12 min and then treated with 3% hydrogen dioxide solution for 10 min. The sections were incubated with the 1:20-diluted anti-CAEV (primary) antibody (MAb CAEV-63, CAEV-Co IgG1, CAEP10A1, VMRD, Pullman, WA, USA) overnight at 4 • C in a humid chamber [31]. After washing in TRIS-buffered saline, the sections were incubated with the 1:150-diluted polyclonal goat anti-mouse immunoglobulins conjugated with horseradish peroxidase (secondary antibody) (Gt a Mo immunoglobulins/HRP, P044701-2, Dako, Agilent Tech, Santa Clara, CA, USA), and then visualized with 3,3 -diaminobenzidine (DAB) (Liquid DAB+, K346811-2, Dako, Agilent Tech., Santa Clara, CA, USA).…”

Section: Histopathological Examinationmentioning

confidence: 99%

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The Prevalence of Histopathological Features of Pneumonia in Goats with Symptomatic Caprine Arthritis-Encephalitis

et al. 2022

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Chronic interstitial pneumonia (CIP) is a main pathology of sheep infected with small ruminant lentivirus (SRLV). Caprine arthritis-encephalitis (CAE) is caused by the same pathogen; however, the presence of CIP has been only occasionally reported in SRLV-infected goats. We carried out a cross-sectional study to determine the prevalence of histopathological lesions indicative of CIP in goats with symptomatic CAE, and to investigate whether CIP was associated with a higher prevalence of other types of pneumonia (purulent bronchopneumonia, fibrinous pleuropneumonia) or bacterial infections. Lung specimens and bronchial swabs were collected for histopathological and bacteriological examination, respectively, from 116 goats from a CAE-affected herd. All goats were euthanized due to severe clinical signs of CAE. The goats were seropositive for SRLV infection in two different ELISAs and the presence of SRLV antigen in the lung tissue was confirmed by immunohistochemistry. Histopathologically, pneumonia of any type was confirmed in 82 goats (70.7%) and CIP was present in 67 goats (57.8%). In most goats, the severity of the histopathological features of pneumonia was mild. Bacteria were detected in bronchial swabs from 73 goats (62.9%). CIP proved to be significantly positively linked to the occurrence of purulent bronchopneumonia (p < 0.001), fibrinous pleuropneumonia (p = 0.001), and of the infection of lungs with bacteria capable of causing pneumonia (p = 0.050). The causal character of these associations should be considered and warrants further investigation.

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Section: Discussionmentioning

confidence: 99%

Section: Histopathological Examinationmentioning

confidence: 99%

The Prevalence of Histopathological Features of Pneumonia in Goats with Symptomatic Caprine Arthritis-Encephalitis

et al. 2022

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Differential gene expression and immune cell infiltration in maedi-visna virus-infected lung tissues

Shi,

Zhang,

Chen

et al. 2024

BMC Genomics

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Background Maedi-visna virus (MVV) is a lentivirus that infects monocyte/macrophage lineage cells in sheep, goats, and wild ruminants and causes pneumonia, mastitis, arthritis, and encephalitis. The immune response to MVV infection is complex, and a complete understanding of its infection and pathogenesis is lacking. This study investigated the in vivo transcriptomic patterns of lung tissues in sheep exposed to MVV using the RNA sequencing technology. Result The results indicated that 2,739 genes were significantly differentially expressed, with 1,643 downregulated genes and 1,096 upregulated genes. Many variables that could be unique to MVV infections were discovered. Gene Ontology analysis revealed that a significant proportion of genes was enriched in terms directly related to the immune system and biological responses to viral infections. Kyoto Encyclopedia of Genes and Genomes analysis revealed that the most enriched pathways were related to virus-host cell interactions and inflammatory responses. Numerous immune-related genes, including those encoding several cytokines and interferon regulatory factors, were identified in the protein-protein interaction network of differentially expressed genes (DEGs). The expression of DEGs was evaluated using real-time polymerase chain reaction and western blot analysis. CXCL13, CXCL6, CXCL11, CCR1, CXCL8, CXCL9, CXCL10, TNFSF8, TNFRSF8, IL7R, IFN-γ, CCL2, and MMP9 were upregulated. Immunohistochemical analysis was performed to identify the types of immune cells that infiltrated MVV-infected tissues. B cells, CD4+ and CD8+ T cells, and macrophages were the most prevalent immune cells correlated with MVV infection in the lungs. Conclusion Overall, the findings of this study provide a comprehensive understanding of the in vivo host response to MVV infection and offer new perspectives on the gene regulatory networks that underlie pathogenesis in natural hosts.

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First Molecular Characterization of Small Ruminant Lentiviruses in Hungarian Goat Population

Ózsvári,

Bárdos,

Moroz-Fik

et al. 2024

Pathogens

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In 2023, a molecular study was conducted on the Hungarian goat population to determine genotypes and subtypes of small ruminant lentiviruses (SRLV) infecting these herds. Ten goat herds seropositive for SRLV infection according to a serosurvey conducted earlier in Hungary were selected, and 135 adult goats (>1 year old) were blood sampled. The two-stage nested real-time PCR (nRT-PCR) was used to detect proviral DNA of SRLV and distinguish between two main viral genotypes (A and B). PCR products were submitted for Sanger dideoxy sequencing, and phylogenetic and molecular evolutionary analyses were conducted on the 200–250 bp-long proviral DNA sequences from the end of long terminal repeat (LTR) region and beginning of gag gene using the MEGA11 software. Reference strains included strains most identical to Hungarian sequences according to the Standard Nucleotide BLAST and prototypic strains for the relevant genotypes and subtypes. Proviral DNA of SRLV was detected in goats from all ten tested herds. A single SRLV genotype was detected in 6 herds—genotype A in three herds and B also in three herds. In four herds, mixed infection with genotypes A and B was confirmed. In total, 110/135 seropositive goats tested positive in the nRT-PCR (81.5%): 49/110 goats (44.5%) for genotype A, 54/110 goats (49.1%) for genotype B, and 7/110 goats (6.4%) for both genotypes. Hungarian sequences belonged to subtypes A1/A18, A2, and subtype B1. This is the first study which shows that Hungarian goats are infected by SRLV belonging to both genotypes A and B.

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Lymphoepithelial Cyst of the Salivary Gland in a Small Ruminant Lentivirus-Positive Goat

Cited by 3 publications

References 24 publications

The Prevalence of Histopathological Features of Pneumonia in Goats with Symptomatic Caprine Arthritis-Encephalitis

The Prevalence of Histopathological Features of Pneumonia in Goats with Symptomatic Caprine Arthritis-Encephalitis

Differential gene expression and immune cell infiltration in maedi-visna virus-infected lung tissues

First Molecular Characterization of Small Ruminant Lentiviruses in Hungarian Goat Population

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