Lymphotropic Administration of Antibacterial Drugs – A Method of Rational Antibiotic Therapy

Abstract

The aim: To explore the possibilities of rationalizing antibacterial therapy by comparative analysis of the accumulation of ceftriaxone administered lymphotropically and intramuscularly. Materials and methods: The research used a method of studying the sensitivity of microorganisms to antibacterial drugs, which is based on the diffusion of antibacterial drugs from the carrier (homogenate of the test organ) in a dense nutrient medium, which, depending on the sensitivity of microorganisms, inhibits the growth of the studied culture and is accompanied by the formation of a growth inhibition zone (GIZ) in the nutrient medium. For control purposes, a commercial disk with ceftriaxone was used, which caused GIZ of microorganisms at the level of 27.05 ± 0.9 mm. Results: The homogenate of organ samples obtained from rabbits administered with the antibiotic lymphotropically, inhibited the growth of test cultures around the discs in all cases. The inhibition was slightly less than the control inoculation, but the result clearly indicated the presence of antibiotics in the test material in the quantity sufficient to inhibit the growth of the pathogen. Concurrently, after intramuscular administration of the antibiotic, biopsy specimens from various growth inhibition organs of test cultures were either not induced at all or were induced in small amounts, which was certainly insufficient for the inhibitory action of the drug administered by this method. Conclusions: The administration of the antibiotic lymphotropically promotes its accumulation in all organs in the quantity sufficient for antibacterial action, which allows us to recommend lymphotropic therapy as a rational method of antibiotic therapy.

Morphofunctional Changes in Lymph Nodes During Intralymphatic Immunostimulation With Various Drugs in a Rat Model

“…The lymphatic system is a major component of the immune system and acts as a secondary circulatory system to drain excess fluid, proteins, and waste products from the extracellular space into the vascular system [1][2][3][4][5]. Lymphatic vessels have been used as potential drug delivery vehicles because certain lipophilic compounds, such as long-chain fatty acids, triglycerides, cholesterol esters, fat-soluble vitamins, and some xenobiotics, are transported through these channels [6][7][8][9][10][11].…”