Lyophilization of
            <i>Brucella melitensis</i>

Heckly, Robert J.; Faunce, Kenneth; Elberg, Sanford S.

doi:10.1128/am.8.1.52-54.1960

Cited by 4 publications

(1 citation statement)

References 3 publications

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“…Long-term preservation was done by lyophilization, in order to obtain safe and stable stock culture. Enterococcus strain was frozen at −20 • C and −80 • C in MRS supplemented with 15% (v/v) glycerol [8].…”

Section: Materials Sugars and Culture Mediamentioning

confidence: 99%

Purification of an l-arabinose isomerase from Enterococcus faecium DBFIQ E36 employing a biospecific affinity strategy

Torres

Manzo

Rubiolo

et al. 2014

Journal of Molecular Catalysis B: Enzymatic

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Section: Materials Sugars and Culture Mediamentioning

confidence: 99%

Purification of an l-arabinose isomerase from Enterococcus faecium DBFIQ E36 employing a biospecific affinity strategy

Torres

Manzo

Rubiolo

et al. 2014

Journal of Molecular Catalysis B: Enzymatic

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Viability and Estimation of Shelf-Life of Bacterial Populations

et al. 1962

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Mathematical concepts associated with the exponential and probit models are developed, and the similarity of the two methods is discussed. Because of its greater flexibility in design, the probit method was used to estimate the shelf-life for four bacterial populations, wet and dry spores of Bacillus anthracis and wet and dry cells of Pasteurella tularensis . On the basis of data gained by storing these organisms at high temperature, the probit method was used to predict the time at which 50% viability would occur for cells stored at 3 C. A plane passing through a three-space showing change in percentage viability of bacteria was formulated by a multiple regression method. With this functional technique, the percentage viability, expressed as a probit, was linearily related to a logarithm of storage time and storage temperature. The use of this method to study the effect of controlled variables on the viability of cells is demonstrated by comparing the effect of viability associated with three additives used prior to drying. The results of the test gave shelf-life estimates which were too low for all additives; however, the order of stability was ranked properly as confirmed by long-term tests.

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Viability of Bacillus popilliae after Lyophilization of Liquid Nitrogen Frozen Cells

1967

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The per cent viability of Bacillus popilliae after lyophilization of liquid nitrogen frozen cells was determined. Lyophilization of 9- to 12-hr cells which had been suspended in 5% sodium glutamate plus 0.5% gum tragacanth, frozen in liquid nitrogen vapor, and dried 4 to 5 hr with the ampoules exposed to room temperature resulted in survival of 64.6% of the original cells. After storage of these lyophilized preparations for 6 months at room temperature, 10.5% of the original cells were still viable.

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Lyophilization of Brucella melitensis

Cited by 4 publications

References 3 publications

Purification of an l-arabinose isomerase from Enterococcus faecium DBFIQ E36 employing a biospecific affinity strategy

Purification of an l-arabinose isomerase from Enterococcus faecium DBFIQ E36 employing a biospecific affinity strategy

Viability and Estimation of Shelf-Life of Bacterial Populations

Viability of Bacillus popilliae after Lyophilization of Liquid Nitrogen Frozen Cells

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