Lyotropic behavior of Gelucire 50/13 by XRD, Raman and IR spectroscopies according to hydration

Hadri, M. El; Achahbar, A.; Khamkhami, J. El; Khelifa, B.; Faivre, Vincent; Abbas, Ouissam; Bresson, Serge

doi:10.1016/j.chemphyslip.2016.05.005

Cited by 14 publications

(8 citation statements)

References 29 publications

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“…As per Rozenberg et al and Hadri et al, the wavenumbers 1104 cm –1 (assigned to the ν(C–O) trans ) and 1121 cm –1 (ν(C–O) gauche ) correspond to the trans and gauche form of the ether bond. Thus, the ratio of the intensities of I 1104 and I 1121 of different MDG samples can be used to represent the change in the relative amount of gauche to trans forms upon aging ( I 1104 / I 1121 = I trans / I gauche ). − The ratio of trans to gauche was found to be higher in the case of the oldest lot compared to the young lots. As shown in Table , a continuous increase in the trans form with higher stability was observed, suggesting an increase in the stable polymorphic form (β form) in MDG samples with aging (B4 to B1).…”

Section: Resultsmentioning

confidence: 99%

Interplay of Aging and Lot-to-Lot Variability on the Physical and Chemical Properties of Excipients: A Case Study of Mono- and Diglycerides

et al. 2021

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The present study investigates the chemical composition governing the physical properties of mono- and diglycerides (MDGs) at the microstructural level, as a function of aging and lot-to-lot variability. The physical structure of the MDG plays a vital role in ameliorating the emulsion stability and is widely explored in diverse research horizons related to the pharmaceutical, cosmetic, and food industries. In an effort to understand the mechanism of emulsion stabilization, physical properties were extensively evaluated in selective commercial lots to determine if there is a correlation between the chemical composition of MDG and physical properties. The solid state of the MDG samples with different aging profiles was characterized using X-ray scattering, differential scanning calorimetry, attenuated total reflection-Fourier transform infrared spectroscopy, and NMR relaxometry. Moreover, the kinetic aspect of solid-state transformation was also evaluated via treating MDG samples with a heat–cool cycle. The chemical composition of MDGs was quantified using a quantitative NMR (qNMR) method. Interestingly, the X-ray scattering results demonstrated a change in the MDG polymorphic form and an increase in the %β content as a function of aging. The increase in the %β content led to the formation of rigid crystal structures of MDG, as evident from the NMR relaxometry. Chemical quantification of isomeric composition revealed chemical composition change as a potentially critical factor responsible for the altered physical structures of MDG with respect to aging and lot-to-lot variability. The findings correlated the solid-state transformation with the change in the chemical composition of the MDG as a combined effect of aging and lot-to-lot variability. This work serves as a basis to better understand the interdependency of the physicochemical properties of MDG. Furthermore, the present work can also be used as guidance for setting up the specifications of MDG, as per the required polymorphic form for a multitude of applications.

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Section: Resultsmentioning

confidence: 99%

Interplay of Aging and Lot-to-Lot Variability on the Physical and Chemical Properties of Excipients: A Case Study of Mono- and Diglycerides

et al. 2021

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“…Multiple studies of the solid-state of GSE-SLN were carried out to gain a deeper insight into their organization. With regard to FT-IR spectra (Figure 3), the peaks at 3435 cm −1 and 1738-1739 cm −1 (Figure 3a-c), and 1732-1737 cm −1 (Figure 3b,d) could be assigned to partially hydrated Gelucire ® 50/13 [14,15], evidencing the external localization of such lipids in the SLN structure.…”

Section: Solid-state Studiesmentioning

confidence: 93%

Solid Lipid Nanoparticles Administering Antioxidant Grape Seed-Derived Polyphenol Compounds: A Potential Application in Aquaculture

et al. 2022

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The supply of nutrients, such as antioxidant agents, to fish cells still represents a challenge in aquaculture. In this context, we investigated solid lipid nanoparticles (SLN) composed of a combination of Gelucire® 50/13 and Precirol® ATO5 to administer a grape seed extract (GSE) mixture containing several antioxidant compounds. The combination of the two lipids for the SLN formation resulted in colloids exhibiting mean particle sizes in the range 139–283 nm and zeta potential values in the range +25.6–43.4 mV. Raman spectra and X-ray diffraction evidenced structural differences between the free GSE and GSE-loaded SLN, leading to the conclusion that GSE alters the structure of the lipid nanocarriers. From a biological viewpoint, cell lines from gilthead seabream and European sea bass were exposed to different concentrations of GSE-SLN for 24 h. In general, at appropriate concentrations, GSE-SLN increased the viability of the fish cells. Furthermore, regarding the gene expression in those cells, the expression of antioxidant genes was upregulated, whereas the expression of hsp70 and other genes related to the cytoskeleton was downregulated. Hence, an SLN formulation containing Gelucire® 50/13/Precirol® ATO5 and GSE may represent a compelling platform for improving the viability and antioxidant properties of fish cells.

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“…confirming that the raw drug was in the stable polymorphic form. Unloaded particles showed bands typical of Gelucire: 3100-3600 cm -1 (broad, stretching of free OH groups), 1738.5 cm -1 (stretching C=O group), 1469.5 cm -1 (C-H deformation of alkyl group), 1113.7 cm -1 (-C-O stretching), and 963.3 cm -1 (double band, characteristic of the polyethylene glycol groups) [24] [27] [28]. MPs and Phy mix present FT-IR spectra similar to the unloaded particles but with some extra band that can be ascribed to the presence of drug, although with lower intensity due to the limited content of drug in the sample (10%).…”

Section: Solid State Characterization Of Ind-loaded Mpsmentioning

confidence: 99%

Exploring the use of spray congealing to produce solid dispersions with enhanced indomethacin bioavailability: In vitro characterization and in vivo study

Bertoni

Albertini

Ferraro

et al. 2019

European Journal of Pharmaceutics and Biopharmaceutics

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Lyotropic behavior of Gelucire 50/13 by XRD, Raman and IR spectroscopies according to hydration

Cited by 14 publications

References 29 publications

Interplay of Aging and Lot-to-Lot Variability on the Physical and Chemical Properties of Excipients: A Case Study of Mono- and Diglycerides

Interplay of Aging and Lot-to-Lot Variability on the Physical and Chemical Properties of Excipients: A Case Study of Mono- and Diglycerides

Solid Lipid Nanoparticles Administering Antioxidant Grape Seed-Derived Polyphenol Compounds: A Potential Application in Aquaculture

Exploring the use of spray congealing to produce solid dispersions with enhanced indomethacin bioavailability: In vitro characterization and in vivo study

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