Lysine-doped polypyrrole/spider silk protein/poly( l -lactic) acid containing nerve growth factor composite fibers for neural application

Zhang, Hong; Wang, Kefeng; Xing, Yifei; Yu, Qian

doi:10.1016/j.msec.2015.06.024

Cited by 50 publications

(32 citation statements)

References 32 publications

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“…22, 23 The injection of NGF is a useful method to stimulate bone formation 14, 24, 25 NGF can also be delivered using various scaffolds, including agarose hydrogel, copolymers (PPy-NSE), poly-lactic-co-glycolic-acid (PLGA) and so on. 26, 27, 28, 29, 30, 31, 32 However, most of the carriers for NGF are hydrogels or porous polymers that can hardly achieve the functional reconstruction of large-scale defects. A combination of NGF with Ca-P ceramics, such as BCP, may hold promise in the therapy of extensively damaged bone.…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Guangxi cobra venom-derived NGF promotes the osteogenic and therapeutic effects of porous BCP ceramic

et al. 2017

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Neuro-osteological interactions have an important role in the regulation of bone metabolism and regeneration. Neuropeptides combined with porous biphasic calcium phosphates (BCP) using protein adsorption may contribute to the acceleration of bone formation. In the present study, we investigated the effect of BCP combined with nerve growth factor (NGF) on the growth of osteoblasts in vitro and the combinational therapeutic effect on the repair of calvarial defects in vivo. NGF was separated and purified from Chinese cobra venom using a simplified three-step chromatography method. BCP combined with NGF exerted a potent effect on osteoblast differentiation, as evidenced by enhanced cell proliferation, increased ALP activity and the up-regulated expression of osteogenesis-related genes and proteins. Further, combinational therapy with BCP and NGF improved calvarial regeneration, which was superior to treatment with therapy alone, as observed using imageological and morphological examination and histological and immunohistochemical staining. The results confirmed the effect of neuro-osteological interactions through combinatorial treatment with NGF and BCP to promote osteogenesis and bone formation, which may provide an effective and economical strategy for clinical application.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Guangxi cobra venom-derived NGF promotes the osteogenic and therapeutic effects of porous BCP ceramic

et al. 2017

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“…Engineered bionic conduit showed promising in vivo results [132]. Research has also reported the development of silk fibroin conduits loaded with nerve growth factors [133][134][135][136][137][138]. Despite the promising results, no silk conduit has yet been FDA approved.…”

Section: N-fibroinmentioning

confidence: 99%

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Pedrosa¹,

Caseiro²,

Santos³

et al. 2017

Scaffolds in Tissue Engineering - Materials, Technologies and Clinical Applications

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Human adult peripheral nerve injuries are a high incidence clinical problem that greatly affects patients' quality of life. Although peripheral nervous system has intrinsic regenerative capacity, this occurs in an incomplete or poorly functional manner. When a nerve fiber loses its continuity with consequent damage of the basal lamina tubes, axon spontaneous regeneration is disorganized and mismatched. These phenomena translate in an inadequate nerve functional recovery and consequent musculoskeletal incapacity. Nerve grafts still remain the gold standard in peripheral injuries treatment. However, this approach contains its disadvantages such as the necessity of primary surgery to harvest the autografts, loss of a functional nerve, donor site morbidity and longer surgery procedures. Therefore, biomaterials and tissue engineering can provide efficient resources and alternatives to nerve injury repair not only by the development of biocompatible structures but also, introducing neurotrophic factors and cellular systems to stimulate optimum clinical outcome. In this chapter, a comprehensive state-of-the art picture of tissue-engineered nerve grafts scaffolds, their application in nerve regeneration along with latest advances in peripheral nerve repair and future perspectives will be discussed, including our own large experience in this field of knowledge.

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“…Moreover, many applications could be achieved by preparation of fibers in other textile forms using knitting, weaving, or braiding since the textile mimics important mechanical characteristics of native tissue. For example, the bundle of parallel fibers could serve as a template in nerve regeneration, braided 3 D scaffold in tendon regeneration or meshed scaffold in cartilage tissue repair, where HA can be naturally found. Nanofibers from various materials are often used for the same applications as scaffolds mimicking more accurately the native tissue .…”

Section: Resultsmentioning

confidence: 99%

“…Scaffolds with a fibrous structure have been intended for engineering of neural tissue, wound healing, oesophageal tissue engineering, bone regeneration, ligament tears healing and osteoarthritis repair . Usually, natural polymers like polysaccharides and ECM derived polypeptides are prefered materials, because they mimic closely the characteristics of the natural ECM and can provide cells with anchoring sites and signals that are essential for the formation of functional tissue .…”

Section: Introductionmentioning

confidence: 99%

Optimization of cell growth on palmitoyl‐hyaluronan knitted scaffolds developed for tissue engineering applications

Sogorkova

Zapotocky

Čepa

et al. 2018

J Biomedical Materials Res

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Polysaccharides meet several criteria for a suitable biomaterial for tissue engineering, which include biocompatibility and ability to support the delivery and growth of cells. Nevertheless, most of these polysaccharides, for example dextran, alginate, and glycosaminoglycans, are highly soluble in aqueous solutions. Hyaluronic acid hydrophobized by palmitic acid and processed to the form of wet-spun fibers and the warp-knitted textile scaffold is water non-soluble, but biodegradable material, which could be used for the tissue engineering purpose. However, its surface quality does not allow cell attachment. To enhance the biocompatibility the surface of palmitoyl-hyaluronan was roughened by freeze drying and treated by different cell adhesive proteins (fibronectin, fibrinogen, laminin, methacrylated gelatin and collagen IV). Except for collagen IV, these proteins covered the fibers uniformly for an extended period of time and supported the adhesion and cultivation of dermal fibroblasts and mesenchymal stem cells. Interestingly, adipose stem cells cultivated on the fibronectin-modified scaffold secreted increasing amount of HGF, SDF-1, and VEGF, three key growth factors involved in cardiac regeneration. These results suggested that palmitoyl-hyaluronan scaffold may be a promising material for various applications in tissue regeneration, including cardiac tissue repair. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1488-1499, 2018.

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Lysine-doped polypyrrole/spider silk protein/poly( l -lactic) acid containing nerve growth factor composite fibers for neural application

Cited by 50 publications

References 32 publications

RETRACTED ARTICLE: Guangxi cobra venom-derived NGF promotes the osteogenic and therapeutic effects of porous BCP ceramic

RETRACTED ARTICLE: Guangxi cobra venom-derived NGF promotes the osteogenic and therapeutic effects of porous BCP ceramic

Scaffolds for Peripheral Nerve Regeneration, the Importance of In Vitro and In Vivo Studies for the Development of Cell-Based Therapies and Biomaterials: State of the Art

Optimization of cell growth on palmitoyl‐hyaluronan knitted scaffolds developed for tissue engineering applications

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