Lysinibacillus contaminans sp. nov., isolated from surface water

Abstract

A Gram-positive-staining, aerobic, endospore-forming bacterium, isolated as a contamination from an enrichment of enteric bacteria from surface water, was studied using a polyphasic taxonomic approach. 16S rRNA gene sequence similarity comparisons revealed that strain FSt3AT was grouped in the genus Lysinibacillus, most closely related to Lysinibacillus xylanilyticus XDB9 T (98.1 %), Lysinibacillus parviboronicapiens BAM-582 T and Lysinibacillus sphaericus DSM 28 T (both 98.0 %). The 16S rRNA gene sequence similarity to other species of the genus Lysinibacillus was ,97.5 %. The allocation to the genus Lysinibacillus was supported by a detailed chemotaxonomic characterization revealing a cell wall containing alanine, glutamic acid, aspartic acid and the diagnostic diamino acid lysine in a molar ratio of 1.6 : 1 : 0.9 : 0.8 (peptidoglycan type A4a), the major menaquinones MK-7 and MK-6, and polar lipids consisting of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unknown phospholipids, one unknown aminophospholipid and one unidentified aminolipid. The major fatty acids were iso-and anteiso-branched fatty acids.

Lysinibacillus alkaliphilus sp. nov., an extremely alkaliphilic bacterium, and emended description of genus Lysinibacillus

“…At the time of writing, the genus Lysinibacillus consisted of eighteen species with validly published names (http://www.bacterio.net/lysinibacillus.html) with Lysinibacillus boronitolerans as the type species. Members of this genus have been isolated from environments such as different types of soil (Ahmed et al , 2007; Miwa et al , 2009; Liu et al , 2013), surface water (Kämpfer et al , 2013), fermented soybean food (Kim et al , 2013), and inner tissues of plants and human beings (Glazunova et al , 2006; Duan et al , 2013). In this study, during an investigation of the diversity of bacterial population of typical sandy loam soil under long-term fertilization, an endospore-forming, Gram-staining-positive bacterium, strain OMN17 T , was isolated and found to have morphological properties consistent with the genus Lysinibacillus .…”

Lysinibacillus antri sp. nov., isolated from cave soil

“…The fatty acid composition of strain SYSU K30002 T varied when compared with L. endophyticus CGMCC 1.15291 T and L. sinduriensis KCTC 13296 T , especially the low amounts of anteiso-C 15 : 0 and anteiso-C 17 : 0 in SYSU K30002 T ( Table 2). Although anteiso-C 15 : 0 was reported as a major fatty acid constituent in most members of the genus Lysinibacillus [3,4] but in L.sphaericus its portion was < 5% [7].…”

“…The genus Lysinibacillus belongs to the family Bacillaceae of the phylum Firmicutes, and was proposed by Ahmed et al [1]. At the time of writing, the genus consisted of 26 recognized species [2] reported from a wide range of environments, such as plants [3], fermented food [4], different soil types [5,6] and contaminant from surface water [7]. During our microbial diversity analysis of cave soil samples in Xingyi county, China, many novel candidate bacteria were isolated, and reported [8].…”

Lysinibacillus acetophenoni sp. nov., a solvent-tolerant bacterium isolated from acetophenone

“…However, some species of the genus Lysinibacillus do not contain phosphatidylethanolamine, but unidentified glycolipids are present as major polar lipids (Jung et al , 2012). To date, there are 21 species (with validly/effectively published names) in the genus Lysinibacillus , with Lysinibacillus varians (Zhu et al , 2014), L. halotolerans (Kong et al , 2014), L. composti (Hayat et al , 2014), ‘ L. fluoroglycofenilyticus ’ (Cheng et al , 2015), L. tabacifolii (Duan et al , 2013), L. chungkukjangi (Kim et al , 2013a), L. contaminans (Kämpfer et al , 2013), ‘ L. jejuensis ’ (Kim et al , 2013b) and L. manganicus (Liu et al , 2013) being recent additions. The names ‘ L. jejuensis ’ and ‘ L. fluoroglycofenilyticus ’ have not yet been validly published.…”