Arcobacter anaerophilus sp. nov., isolated from an estuarine sediment and emended description of the genus Arcobacter Two strains (JC83, JC84 T ) of obligately anaerobic, H 2 S-producing bacteria were isolated from estuarine sediment samples collected from Gangasagar, West Bengal, India. Cells were Gramstain-negative, non-motile rods. Both strains were positive for oxidase, negative for catalase, hydrolysed casein, reduced nitrate and utilized citrate. Both strains grew chemoorganoheterotrophically with optimal pH of 7-8 (range 7-10) and at 30 6C (range 25-37 6C). C 16 : 1 v7c, C 18 : 1 v7c, C 16 : 0 and C 12 : 0 were the major fatty acids of both strains with minor amounts of C 14 : 0 , C 12 : 0 3-OH and C 18 : 0 . Polar lipids of both strains included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, phosphatidylcholine, phosphatidylinositol, an unidentified aminolipid (AL2), an unidentified phospholipid (PL2) and an unidentified lipid (L3). MK-6 was the major respiratory quinone. The DNA G+C content of strains JC83 and JC84 T was 25.0 and 24.6 mol%, respectively. The strains showed DNA reassociation .85 % (86.0±0.5 %) (based on DNA-DNA hybridization). Based on 16S rRNA gene sequence analysis, both strains were identified as belonging to the family Campylobacteraceae of the class Epsilonproteobacteria with Arcobacter marinus CL-S1 T (95.4 % sequence similarity) as their closest phylogenetic neighbour. On the basis of morphological, physiological and chemotaxonomic characteristics as well as phylogenetic analysis, strains JC83 and JC84 T are considered to represent a novel species, for which the name Arcobacter anaerophilus sp. nov. is proposed. The type strain is JC84 T (5KCTC 15071 T 5MTCC 10956 T 5DSM 24636 T ). An emended description of the genus Arcobacter is provided.

No abstract

No abstract

A Gram-stain-positive, oxidase-negative, starch-hydrolysing, actinobacterium (strain JC82 T ) was isolated from a soda lake in Lonar, India. Based on 16S rRNA gene sequence similarity studies, strain JC82 T belonged to the genus Georgenia and was most closely related to Georgenia muralis 1A-C T (96.8 %) and other members of the genus Georgenia (,96.5 %). The DNA G+C content of strain JC82 T was 73.4 mol%. The cell-wall amino acids were alanine, glutamic acid and lysine with peptidoglycan type A4a. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides, an unidentified lipid (L1) and an unidentified glycolipid (GL3). The predominant isoprenoid quinone was menaquinone MK-8(H 4 ). Anteiso-C 15 : 0 was the predominant fatty acid and significant proportions of iso-C 14 : 0 , C 14 : 0 , C 16 : 0 , iso-C 15 : 0 and iso-C 16 : 0 were also detected. Strain JC82 T produced thermostable alkaline amylase. The results of physiological and biochemical tests allowed a clear phenotypic differentiation of strain JC82 T from all other members of the genus Georgenia. Based on these data, strain JC82 T represents a novel species of the genus Georgenia, for which the name Georgenia satyanarayanai sp. nov. is proposed. The type strain is JC82 T (5KCTC 19802 T 5NBRC 107612 T ).The alkaline Lonar Lake (19 u 589 360 N, 76 u 309 300 E) in India is a unique basaltic rock meteorite impact crater, ranking third in the world in terms of alkalinity. The lake is rich in bacterial diversity (Joshi et al., 2008;Wani et al., 2006), although at the time of writing, only two bacterial species isolated from the lake had validly published names (Indibacter alkaliphilus and Nitritalea halalkaliphila; Anil Kumar et al., 2010a, b). In this report, we describe a novel actinobacterium, strain JC82 T belonging to the genus Georgenia which was isolated from a sediment sample from Lonar Lake. The genus Georgenia was first proposed by Altenburger et al. (2002) with Georgenia muralis as the type species. At the time of writing, the genus Georgenia comprised six recognized species, including the recently described Georgenia daeguensis (Woo et al., 2012). The members of this genus are Gram-stainpositive, aerobic or facultative anaerobic, non-sporulating, oxidase-and catalase-positive, motile or non-motile bacteria with MK-8(H4) as the predominant menaquinone, anteiso-C 15 : 0 as the major fatty acid, lysine as the diagnostic cell wall amino acid and a polar lipid profile with diphosphatidylglycerol and phosphatidylglycerol as major components (Tang et al., 2010). Strain JC82 T was isolated from a sediment sample collected from Lonar Lake in Maharashtra, India, using nutrient agar (Hi-Media) under aerobic conditions. Pure cultures were lyophilized and preserved at 4 u C. Morphological properties such as cell shape, cell size and motility were observed by phase-contrast light microscopy (BH-2; Olympus). The pH and temperature ranges for growth were examined in nutrient broth. Salt tolerance tests...

Two bacterial strains (JC109 T and JC261) were isolated from a sediment sample collected from a shrimp cultivation pond in Tamil Nadu (India). Cells were Gram-stain-negative, motile rods. Both strains were positive for catalase and oxidase, hydrolysed Tween 80, and grew chemoorganoheterotrophically with an optimal pH of 6 (range pH 4-9) and at 30 6C (range 25-40 6C).