Lysis of fld‐3 friend erythroleukemia cellsin vitroandin vivo: Effect of⁸⁹sr treatment and friend virus infection

Abstract

The effector cells from non-immunized mice capable of lysing 51Cr-labelled FLD-3 BALB/c Friend virus-induced erythroleukemia cells in vitro and cells capable of clearing FLD-3 cells labelled with 5-iodo-2'-deoxyuridine-125I (125IdUrd) from the lungs in vivo were characterized and compared with natural killer (NK) cells reactive against YAC-I lymphoma cells. Unlike NK cells, the cells capable of lysing FLD-3 cells in vitro were insensitive to antibodies directed against NK-2.1 or Thy-1.2 antigens (plus complement) and to pretreatment of mice in vivo with silica particles, 89Sr or estradiol. Heat-killed C. parvum organism stimulated anti-FLD-3 effector cells without changing the slow rate (24 h) of lysis in vitro. The ability to clear FLD-3 and YAC-1 cells from the lung was normal and defective, respectively, in C57BL/6-bg/bg(beige) mice and in mice pretreated with 89Sr or estradiol. We conclude that natural cytotoxic (NC) cells lyse FLD-3 cells, Fv-2, which regulates resistance to leukemia induction by Friend virus, does not regulate NC(FLD-3) activity, and the virus does not affect NC(FLD-3) activity during the first several days of infection of normal genetically susceptible mice. However, infection of 89Sr-treated mice inhibits NC(FLD-3) function owing to the activation of suppressor cells. These data suggest (but do not prove) that effector cells similar or identical to NC(FLD-3) cells may function in vivo to resist the proliferation/survival of certain leukemia cells.

The Effect of Immunization with Killed Tumor Cells, with/Without Feeding ofEchinacea purpureain an Erythroleukemic Mouse Model

“…During the early period after tumor onset (9 days) in immunized mice, splenic NK cell numbers increased even more profoundly to 40-60 times those found in unimmunized, leukemic mice (Currier and Miller, 2001;Miller et al, 1992), supporting the well-established fact that erythroleukemia antigens are powerful stimulants for NK cells (Afifi et al, 1986;Hagner, 1984;Iorio et al, 1986;Lust et al, 1984;Neri et al, 1989;Thurlow et al, 1986). In the bone marrow, however, NK cells increased only twofold, again demonstrating the resistance of bone marrow based cells (T, B, or NK) to the systemic presence of foreign antigens.…”

Identification of a subset of murine natural killer cells that mediates rejection of Hh-1d but not Hh-1b bone marrow grafts.

“…In addition to reactivity to allogeneic stem cells, NK cells are known to interact with autologous stem cells both in vitro and in vivo (23) . Thomson et al (24) reported that NK cells are capable of eliminating syngeneic stem cells in vivo after certain viral infections .…”

Influence of an Interferon Inducer on Bone Marrow Transplant Reconstitution in Irradiated, Leukemic Mice: Elevated Natural Killer Cell Numbers and Improved Life Span

“…However, the leukemia, which is the subject of the present study, is known to be NK sensitive and T cell insensitive [47][48][49]. The tumor is non-responsive to the immunostimulants generated in vivo in the presence of poly I:C and, in fact, this leukemia appears to proliferate only in response to internal autocrine mechanisms [50,51], involving only epo and epoR.…”