Lysobacter profundi sp. nov., isolated from freshwater sediment and reclassification of Lysobacter panaciterrae as Luteimonas panaciterrae comb. nov.

Jin, Chun-Zhi; Song, Xiuli; Sung, Yun Ju; Jin, Feng-Jie; Li, Taihua; Oh, Hee‐Mock; Lee, Hyung-Gwan; Jin, Long

doi:10.1099/ijsem.0.004253

“…The major components of the cellular fatty acids of strain SG-8 T included iso-C 15 : 0 (20.6 %), summed feature 9 (iso-C 17 : 1 ω 9 c and/or 10-methyl C 16 : 0 , 20.2 %), iso-C 16 : 0 (19.3 %), summed feature 3 (C 16 : 1 ω 6 c and/or C 16 : 1 ω 7 c ; 8.0 %), iso-C 17 : 0 (7.8 %), iso-C 11 : 0 3OH (6.6 %) and iso-C 11 : 0 (5.8 %). According to , the fatty acid profile of strain SG-8 T was similar to those of the four reference strains and also other Lysobacter species [15–17], indicating that strain SG-8 T belongs to the genus Lysobacter . Nevertheless, some fatty acid constituents of strain SG-8 T showed differences in specific proportions, such as less iso-C 16 : 0 and more iso-C 17 : 0 , summed feature 3 and summed feature 9, under synchronous experimental conditions.…”

Section: Full-textmentioning

confidence: 92%

Lysobacter penaei sp. nov., isolated from intestinal content of a Pacific white shrimp (Penaeus vannamei)

Xu

¹

,

Li

²

,

Zhang

³

et al. 2019

International Journal of Systematic and Evolutionary Microbiology

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The polyphasic taxonomic approach was used to characterize a novel bacteria strain, designated SG-8T, which was isolated from intestinal content of a Pacific white shrimp (Penaeus vannamei). Cells were Gram-stain-negative, aerobic, non-gliding rods. Growth occurred at 10–45 °C (optimum, 20–30 °C), pH 5.0–10.0 (optimum, 6.0–7.0) and in 0–6.0 % (w/v) NaCl (optimum, 0–4.0 %). The 16S rRNA gene sequence of strain SG-8T showed the highest sequence similarity to Lysobacter maris KMU-14T (98.6 %). On phylogenetic trees, strain SG-8T formed a stable cluster with Lysobacter maris KMU-14T, Lysobacter alkalisoli SJ-36T, Lysobacter spongiae 119BY6-57T and Lysobacter aestuarii S2-CT. The average nucleotide identity and digital DNA–DNA hybridization values between strain SG-8T and the four reference type strains listed above were 83.3, 82.3, 83.5, 83.3% and 22.8, 22.7, 22.7, 22.9 %, respectively. The major fatty acids (>5 %) were iso-C15 : 0, summed feature 9 (iso-C17 : 1 ω9c and/or 10-methyl C16 : 0), iso-C16 : 0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c), iso-C17 : 0, iso-C11 : 0 3OH and iso-C11 : 0. Ubiquinone-8 (Q-8) was the only respiratory quinone. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The DNA G+C content was 68.8 mol%. Based on the results of genomic, phylogenetic, phenotypic and chemotaxonomic analyses, strain SG-8T represents a novel species of the genus Lysobacter , for which the name Lysobacter penaei sp. nov. is proposed. The type strain is SG-8T (=GDMCC 1.1817T=KACC 21942T).

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“…[3] proposed the reclassification of Luteimonas tolerans under the genus Lysobacter . In addition, based on the phylogenetic relationships together with phenotypic characteristics and fatty acid compositions, Lysobacter panaciterrae was reclassified as a member of the genus Luteimonas [4]. At the time of writing, the genus Luteimonas includes 27 recognized species with validly published names (https://lpsn.dsmz.de/genus/luteimonas) [5], distributed in different environments such as soil [6, 7], seawater [8], stratum water [9], plant leaves [10], sediment samples [11, 12] and food waste [13], and show diverse environmental adaptability and unique biological functions.…”

Section: Introductionmentioning

confidence: 99%

Luteimonas galliterrae sp. nov., isolated from poultry farm soil

Shen

¹

,

An

²

,

He

³

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

0

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Strain SX5T was isolated from the soil of a poultry farm in Shanxi Province, PR China. The isolate was a Gram-stain-negative, rod-shaped, non-flagellated, and yellow bacterium. Growth occurred at 20–37 °C (optimum, 28 °C), pH 6.0–10.0 (optimum, pH 8.0) and 0–1 % NaCl (optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SX5T was related to members of the genus Luteimonas , and close to Luteimonas gilva H23T (97.9 %), Luteimonas cucumeris Y4T (97.9 %), Luteimonas aquatica RIB1-20T (96.8 %), Luteimonas notoginsengisoli SYP-B804T (96.4 %) and Luteimonas panaciterrae Gsoil 068T (96.1 %). The major cellular fatty acids of strain SX5T were iso-C16 : 0, iso-C17 : 1 ω9c, iso-C15 : 0 and iso-C11 : 0 3OH. The sole isoprenoid quinone was ubiquinone Q-8, and the major polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Genome analyses revealed that strain SX5T had a genome size of 3.6 Mbp with a G+C content of 65.7 mol% and contained abundant carbohydrate-active enzyme genes and three putative distinct biosynthetic gene clusters, suggesting that it may have great potential to degrade and utilize complex biological organic matter and produce special secondary metabolites. Comparative genomic analyses clearly separated strain SX5T from the known species of the genus Luteimonas based on average nucleotide identity and digital DNA–DNA hybridization values below the thresholds for species delineation. Based on its phenotypic, genotypic properties and phylogenetic inference, strain SX5T represents a novel species in the genus Luteimonas , for which the name Luteimonas galliterrae sp. nov. is proposed. The type strain is SX5T (=GDMCC 1.2162T=KCTC 82443T=JCM 34401T).

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“…The major fatty acids include iso-C 15 : 0 , iso-C 16 : 0 and summed feature 9 (iso-C 17 : 1 ω 9 c and/or C 16 : 0 10-methyl) [6, 7]. Phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol are the major polar lipids [2–9]. Members of the genus Lysobacter are isolated from a wide variety of habitats, including rhizosphere of pepper, plateau soil, saline-alkaline soil, lake sediment, oil-contaminated soil, iron-mined soil, deep-sea sponge, anaerobic granules, greenhouse soils, antarctic freshwater lake, marine sediment, activated sludge, fallow farmland soil and kaolin slurry [2–21].…”

Section: Introductionmentioning

confidence: 99%

“…Ubiquinone-8 is the predominant respiratory quinone. A high DNA G+C content with ranging from 61.7 to 71.5 mol% is described in the members of the genus Lysobacter [3–5]. The major fatty acids include iso-C 15 : 0 , iso-C 16 : 0 and summed feature 9 (iso-C 17 : 1 ω 9 c and/or C 16 : 0 10-methyl) [6, 7].…”

Section: Introductionmentioning

confidence: 99%

“…Phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol are the major polar lipids [2–9]. Members of the genus Lysobacter are isolated from a wide variety of habitats, including rhizosphere of pepper, plateau soil, saline-alkaline soil, lake sediment, oil-contaminated soil, iron-mined soil, deep-sea sponge, anaerobic granules, greenhouse soils, antarctic freshwater lake, marine sediment, activated sludge, fallow farmland soil and kaolin slurry [2–21]. Members of the genus Lysobacter produce a range of extracellular enzymes and other metabolites with bacteriostatic or bactericidal activity against bacteria, fungi, oomycetes and nematodes, which have been used to develop antifungal biocontrol against plant pathogens and antibiotics against human pathogens [22–28].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lysobacter selenitireducens sp. nov., isolated from river sediment

Mao

¹

,

Li

²

,

Guo

³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-negative, yellow-pigmented, motile, flagellated and rod-shaped bacterium, designated as 13AT, was isolated from a river sediment sample of Fuyang River in Hengshui City, Hebei Province, PR China. Strain 13AT grew at 10–37 °C (optimum, 30 °C), at pH 5.0–11.0 (optimum, pH 7.0) and at 0–7 % (w/v) NaCl concentration (optimum, 0 %). Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain 13AT belongs to the genus Lysobacter , and was most closely related to Lysobacter spongiicola DSM 21749T (97.8 %), Lysobacter concretionis DSM 16239T (97.5 %), Lysobacter daejeonensis GIM 1.690T (97.3 %) and Lysobacter arseniciresistens CGMCC 1.10752T (96.9 %). Meanwhile, the type species Lysobacter enzymogenes ATCC 29487T was selected as a reference strain (95.2 %). The genomic size of strain 13AT was 3.0 Mb and the DNA G+C content was 69.0 %. The average nucleotide identity values between strain 13AT and each of the reference type strains L. spongiicola DSM 21749T, L. concretionis DSM 16239T, L. daejeonensis GIM 1.690T, L. arseniciresistens CGMCC 1.10752T and L. enzymogenes ATCC 29487T were 75.9, 76.1, 77.7, 78.0 and 73.2 %, respectively. The digital DNA–DNA hybridization values between strain 13AT and each of the reference type strains were 21.7, 22.2, 21.9, 22.7 and 23.2 %, respectively. The average amino acid identity values between strain 13AT and each of the reference type strains were 72.5, 72.9, 72.3, 75.0 and 69.2 %, respectively. The major fatty acids were iso-C15 : 0, iso-C16 : 0 and summed feature 9 (iso-C17 : 1 ω9c and/or C16 : 0 10-methyl). The sole respiratory quinone was identified as ubiquinone-8. The polar lipid profile contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid, an unidentified lipid, four unidentified phospholipids and two unidentified glycolipids. Based on the phenotypic, physiological, phylogenetic and chemotaxonomic data, strain 13AT represents a novel species of the genus Lysobacter , for which the name Lysobacter selenitireducens sp. nov. is proposed. The type strain is 13AT (=JCM 34786T=GDMCC 1.2722T).

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Lysobacter profundi sp. nov., isolated from freshwater sediment and reclassification of Lysobacter panaciterrae as Luteimonas panaciterrae comb. nov.

Cited by 16 publications

References 41 publications

Lysobacter penaei sp. nov., isolated from intestinal content of a Pacific white shrimp (Penaeus vannamei)

Lysobacter penaei sp. nov., isolated from intestinal content of a Pacific white shrimp (Penaeus vannamei)

Luteimonas galliterrae sp. nov., isolated from poultry farm soil

Lysobacter selenitireducens sp. nov., isolated from river sediment

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