Lysophosphatidylcholine produced by the phospholipase A2 isolated from Lachesis muta snake venom modulates natural killer activity as a protein kinase C effector

Fuly, André Lopes; Machado, Alexandre Légora; Castro, Priscila da Silva; Abrahão, Agessandro; Redner, Paulo; Lopes, Ulisses Gazos; Guimarães, Jorge A.; Koatz, Vera Lúcia Gonçalves

doi:10.1016/j.toxicon.2007.04.008

Cited by 22 publications

(18 citation statements)

References 39 publications

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“…7), and lyso-PC (C18:1) (No. 8) are glycerophospholipids, which are important components of all cell membranes (Fuly et al, 2007). Phospholipase A2 can be activated during the breakdown of the membrane, which results in further decreases in the generation of lyso-PCs (Glukhova et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Metabolomics Study of the Biochemical Changes in the Plasma of Myocardial Infarction Patients

et al. 2018

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Myocardial infarction (MI) is a common and multifactorial disease that has the highest morbidity and mortality in the world. Although a number of physiological, pathological, and functional parameters have been investigated, only scarce information regarding the changes of small metabolites in the plasma has been reported, and this lack of information may cause poor MI diagnosis and treatment. In the present study, we aimed to investigate the metabolic profiles of plasma samples from MI patients to identify potential disease biomarkers and to study the pathology of MI. Metabolic profiles of the plasma of 30 MI patients and 30 controls were obtained using ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry. The resulting data were processed using pattern recognition approaches, including principal component analysis and partial least squares-discriminant analysis, to identify the metabolites that differed between the groups. Significant differences in the plasma levels of the following 10 metabolites were observed in the MI patients compared with the controls: phosphatidylserine, C16-sphingosine, N-methyl arachidonic amide, N-(2-methoxyethyl) arachidonic amide, linoleamidoglycerophosphate choline, lyso-PC (C18:2), lyso-PC (C16:0), lyso-PC (C18:1), arachidonic acid, and linoleic acid. The changes in these 10 biomarkers indicated perturbations of energy metabolism, phospholipid metabolism, and fatty acid metabolism in the MI patients. These findings hold promise to advance the treatment, diagnosis, and prevention of MI.

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Section: Discussionmentioning

confidence: 99%

Metabolomics Study of the Biochemical Changes in the Plasma of Myocardial Infarction Patients

et al. 2018

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“…Among these hyaluronidase is responsible for the spreading of poison (da Silveira et al, 2007a) and phospholipase D, referred to in the literature as sphingomyelinase D or phosphodiesterase D due to its ability to hydrolyze sphingomyelin (da Silva et al, 2004) are the main enzymes described. In this work it was shown that the venom of L. intermedia presents sphingomyelinase activity in high concentrations, but does not show phospholipase A 2 activity, as demonstrated for venoms of different species of snakes (Fuly et al, 2007;Strauch et al, 2013). We would have expected a more linear response, but the sphingomyelinase D activity observed only in the highest concentration (100ug/mL) can be explained by the use of venom pool of different spiders Loxosceles intermedia diluted in physiological saline, being this concentration related to the total protein, it is not possible to affirm the exact concentration of the enzyme sphingomyelinase in relation to protein concentration tested.…”

Section: Discussionmentioning

confidence: 64%

Pattern of inflammatory response to Loxosceles intermedia venom in distinct mouse strains: A key element to understand skin lesions and dermonecrosis by poisoning

Ribeiro

Oliveira

Monteiro-Machado

et al. 2015

Toxicon

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“…Moreover, the toxic action of lysolecithin formed by the action of PLA 2 s from other sources of venoms has also been investigated. A PLA 2 isolated from L. muta venom modulates natural killer activity by the protein kinase C pathway [ 53 ]. B. jararaca venom contains several isoenzymes of PLA 2 , serine proteases and metalloproteases that cause edema, coagulation and hemorrhage, respectively.…”

Section: Discussionmentioning

confidence: 99%

The red seaweed Plocamium brasiliense shows anti-snake venom toxic effects

Silva¹,

Domingos²,

Fonseca³

et al. 2015

J Venom Anim Toxins incl Trop Dis

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BackgroundSnakebite is considered a neglected tropical disease by the World Health Organization. In Brazil, about 70% of the envenomation cases are caused by Bothrops snakes. Its venom may provoke hemorrhage, pain, necrosis, hemolysis, renal or cardiac failure and even death in victims. Since commercial antivenom does not efficiently neutralize the local toxic effects of venoms, natural products have been tested in order to provide alternative or complementary treatment to serum therapy. Therefore, the present study aimed to evaluate the ability of the seaweed Plocamium brasiliense and its active derivatives to neutralize hemorrhagic, edematogenic, hemolytic, coagulant and proteolytic activities of B. jararaca venom.MethodsSpecimens of P. brasiliense were collected in Rio de Janeiro state, Brazil, dried and submitted to oil extraction using four solvents of increasing polarities, n-hexane (HEX), dichloromethane (DCM), ethyl acetate (ETA) and hydroalcoholic solution (HYD). The solvents were evaporated, yielding HEX, DCM, ETA and HYD extracts. Further, all extracts were dissolved in dimethylsulfoxide. In addition, two monoterpenes (8-bromo-3,4,7-trichloro-3,7-dimethyl-1E, 5E-octadiene and 1,8-dibromo-3,4,7-trichloro-3,7-dimethyl-1E, 5E-octadiene) and a cholesterol fraction were isolated from the extract of P. brasiliense prepared in hexane. Algal samples were incubated for 30 minutes with B. jararaca venom, and then tested for lethality; hemorrhagic, edematogenic, hemolytic, coagulant and proteolytic effects.ResultsMost of the algal extracts inhibited the toxic effects with different potencies. The DCM extract was the most effective, since it inhibited all types of toxic activity. On the other hand, the HYD extract failed to inhibit any effect. Moreover, the isolated products inhibited proteolysis and protected mice from hemorrhage in 30% of the cases, whereas 8-bromo-3,4,7-trichloro-3,7-dimethyl-1E, 5E-octadiene inhibited 100% and 20% of the hemorrhagic and proteolytic activities, respectively. None of the algal products were toxic to mice.ConclusionSeaweeds may be a promising source of inhibitors against toxic effects caused by B. jararaca envenomation, which may contribute to antivenom treatment.

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Lysophosphatidylcholine produced by the phospholipase A2 isolated from Lachesis muta snake venom modulates natural killer activity as a protein kinase C effector

Cited by 22 publications

References 39 publications

Metabolomics Study of the Biochemical Changes in the Plasma of Myocardial Infarction Patients

Metabolomics Study of the Biochemical Changes in the Plasma of Myocardial Infarction Patients

Pattern of inflammatory response to Loxosceles intermedia venom in distinct mouse strains: A key element to understand skin lesions and dermonecrosis by poisoning

The red seaweed Plocamium brasiliense shows anti-snake venom toxic effects

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