Lysophosphatidylcholine promotes intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 expression in human umbilical vein endothelial cells via an orphan G protein receptor 2-mediated signaling pathway

Zhang, Qian; Zhang, Wei; Liu, Jing; Yang, Haisen; Hu, Yuxia; Zhang, Mengdi; Bai, Tuya; Chang, Fuhou

doi:10.1080/21655979.2021.1956671

Cited by 11 publications

(6 citation statements)

References 55 publications

(62 reference statements)

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“…Icam1 (Inter cellular adhesion molecule-1) is an adhesion receptor found in leukocytes and endothelial cells. Previous reports have shown that upregulation of both Icam1 and Vcam1 had a positive correlation with the destruction of capillaries and tubular cells in kidney rejection ( Vitoria et al, 2019 ; Zhang et al, 2021 ). On one hand, adhesion molecules serve as infiltration mediators; on the other hand, adhesion molecules may also be a costimulatory signal for T-cell activation by antigen-presenting cells ( Wang et al, 2018 ).…”

Section: Discussionmentioning

confidence: 80%

Novel targets in renal fibrosis based on bioinformatic analysis

Yuan

Xiong

et al. 2022

Front. Genet.

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Background: Renal fibrosis is a widely used pathological indicator of progressive chronic kidney disease (CKD), and renal fibrosis mediates most progressive renal diseases as a final pathway. Nevertheless, the key genes related to the host response are still unclear. In this study, the potential gene network, signaling pathways, and key genes under unilateral ureteral obstruction (UUO) model in mouse kidneys were investigated by integrating two transcriptional data profiles.Methods: The mice were exposed to UUO surgery in two independent experiments. After 7 days, two datasets were sequenced from mice kidney tissues, respectively, and the transcriptome data were analyzed to identify the differentially expressed genes (DEGs). Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were executed. A Protein-Protein Interaction (PPI) network was constructed based on an online database STRING. Additionally, hub genes were identified and shown, and their expression levels were investigated in a public dataset and confirmed by quantitative real time-PCR (qRT-PCR) in vivo.Results: A total of 537 DEGs were shared by the two datasets. GO and the KEGG analysis showed that DEGs were typically enriched in seven pathways. Specifically, five hub genes (Bmp1, CD74, Fcer1g, Icam1, H2-Eb1) were identified by performing the 12 scoring methods in cytoHubba, and the receiver operating characteristic (ROC) curve indicated that the hub genes could be served as biomarkers.Conclusion: A gene network reflecting the transcriptome signature in CKD was established. The five hub genes identified in this study are potentially useful for the treatment and/or diagnosis CKD as biomarkers.

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Section: Discussionmentioning

confidence: 80%

Novel targets in renal fibrosis based on bioinformatic analysis

Yuan

Xiong

et al. 2022

Front. Genet.

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“…These cells were cultured and grown in 2% serum endothelial growth media (EGM2) (Lonza, Switzerland) supplied with FBS (10%, v/v) and antibiotics at 37°C in a humidified 5% CO 2 incubator. Cells were exposed to ox-LDL (100 µg/ml) (Sigma-Aldrich, USA) in the inclusion or exclusion 1 and 2 μM Rotigotine (Topscience, Shanghai, China) for another 24 hours [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

“…In ECs, ox-LDL causes endothelial adhesion genes implicated in atherogenesis and endothelial dysfunction such as ICAM-1, monocyte chemoattractant protein-1 (MCP-1), and VCAM-1 to be expressed [ 22 , 23 ]. Although macrophages normally express few receptors for normal LDL, they can take up ox-LDL by ways of scavenger receptors.…”

Section: Introductionmentioning

confidence: 99%

Rotigotine protects against oxidized low-density lipoprotein(ox-LDL)-induced damages in human umbilical vein endothelial cells(HUVECs)

Kang

Fan

et al. 2021

Bioengineered

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Rotigotine is a non-ergoline dopamine agonist that has been licensed for the treatment of Parkinson’s disease. Cardiovascular diseases are the world’s leading cause of death. Ox-LDL- induced endothelial damages are involved in the initiation and progression of cardiovascular diseases. In this study, we assessed the beneficial properties of Rotigotine on ox-LDL-induced insults to HUVECs to highlight its potential use in the treatment of cardiovascular diseases. Our findings show that Rotigotine suppresses the expressions of low-density lipoprotein receptor (LDL-R), proprotein convertase subtilisin/kexin type 9 (PCSK-9), and sterol regulatory element-binding protein (SREBP-2). It also inhibits ox-LDL-induced cholesterol accumulation in endothelial cells (ECs), improves U937 monocytes adhesion, and decreases the representation of NADPH oxidase (NOX-4) and generation of reactive oxygen species (ROS) in endothelial cells (ECs). Furthermore, Rotigotine inhibited the expressions of both vascular cellular adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in HUVECs and had anti-inflammatory efficacy in ox-LDL-induced cells by inhibiting the expressions of pro-inflammatory cytokines. Notably, Rotigotine inhibits the activation of nuclear factor-kappaB (NF-κB) by preventing nuclear translocation of NF-κB p65 and reducing the luciferase activity of NF-κB reporter. We, therefore, conclude that these effects of Rotigotine on HUVECs suggest that it may play a therapeutic role in cardiovascular diseases.

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“…no. sc-5203; Santa Cruz Biotechnology, Santa Cruz, CA, USA) were visualized through strengthened the chemiluminescence (ECL, Amersham Biosciences Corp, NJ, USA), and the analysis of relative band intensity was conducted with ImageJ version 1.46 (National Institutes of Health, Bethesda, MD, USA) [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

Protective effects of Schisandrin B against D-GalN-induced cell apoptosis in human hepatocyte (L02) cells via modulating Bcl-2 and Bax

et al. 2021

Bioengineered

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Schisandrin B is a dibenzocyclooctadiene derivative extracted fromSchisandra chinensis (Turcz.) Baill., that exhibits anti-oxidation, anti-inflammation, anti-tumor and hepatoprotective activities. To understand the hepatoprotective mechanism of schisandrin B, this study investigated the efficacy of schisandrin B on L02 cells after treatment with D-GalN. Following pretreatment with 40 μM schisandrin B, L02 cells were stimulated with 40 mM D-GalN. Cell viability, apoptosis, the expression levels of genes associated with apoptosis, and the intracellular oxidative stress indexes were measured. The viability of L02 cells was determined using MTT assay, and the Annexin V-FITC/PI assay kit was utilized for the assessment of apoptosis. The activities of GSH-Px and SOD, the level of MDA were assessed, separately, using relative detection kits. Moreover, RT-PCR as well as Western blot was applied to measure the mRNA and protein expression of Bax and Bcl-2. The results indicated that schisandrin B significantly prevented D-GalN-induced oxidative damage in L02 cells (P<0.05), decreased GSH-Px and SOD activities (P<0.05), increased MDA content (P<0.05). Furthermore, schisandrin B inhibited D-GalN-induced apoptosis in L02 cells (P<0.05), regulated the expression of Bax and Bcl-2 (P<0.05). The results indicated that schisandrin B decreased the D-GalN-induced intracellular oxidative stress indexes generation, and inhibited the down-regulation of Bcl-2 and up-regulation of Bax induced by D-GalN. In conclusion, schisandrin B was shown to exert protective effect against oxidative damage of L02 cells, which, in part, was achieved by regulating the mRNA and protein levels of Bax and Bcl-2.

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Lysophosphatidylcholine promotes intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 expression in human umbilical vein endothelial cells via an orphan G protein receptor 2-mediated signaling pathway

Abstract: Chang (2021) Lysophosphatidylcholine promotes intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 expression in human umbilical vein endothelial cells via an orphan G protein receptor 2-mediated signaling pathway, Bioengineered, 12:1, 4520-4535,

Cited by 11 publications

References 55 publications

Novel targets in renal fibrosis based on bioinformatic analysis

Novel targets in renal fibrosis based on bioinformatic analysis

Rotigotine protects against oxidized low-density lipoprotein(ox-LDL)-induced damages in human umbilical vein endothelial cells(HUVECs)

Protective effects of Schisandrin B against D-GalN-induced cell apoptosis in human hepatocyte (L02) cells via modulating Bcl-2 and Bax

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