Lysosomal Hydrolases and Inflammatory Materials

Goldstein, Ira M.

doi:10.1007/978-1-4684-0745-7_3

Cited by 13 publications

(4 citation statements)

References 160 publications

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“…Thus human neutrophils contain granule-associated enzymes capable of hydrolyzing a wide variety of both natural and synthetic substrates, including simple and complex polysaccharides, oligopeptides, polypeptides, and phosphate esters. The roles played by these enzymes in maintaining normal host defense and in mediating inflammation have been the subject of several reviews (Goldstein, 1973(Goldstein, , 1976Babior, 1978;Weissmann, 1982).…”

Section: The Lysosomal System Of Neutrophilsmentioning

confidence: 98%

Neutrophil Degranulation

Goldstein

1984

Regulation of Leukocyte Function

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Section: The Lysosomal System Of Neutrophilsmentioning

confidence: 98%

Neutrophil Degranulation

Goldstein

1984

Regulation of Leukocyte Function

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“…Most ofthe injury is caused by the constituents of PMN lysosomes. These compounds are capable of digesting connective tissue components, increasing vascular permeability, and activating other mediators of inflammation such as kinins (10,22).…”

Section: Discussionmentioning

confidence: 99%

“…During phagocytosis, polymorphonuclear leukocytes (PMNs) release lysosomal constituents into the surrounding medium (12,15). These compounds have been implicated as mediators of the inflammatory response and may be involved in the ensuing tissue injury (10,15,22). Several studies (20; W. P. McArthur, N. S. Taichman, A. Stephenson, and R. Negron, J. Dent.…”

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confidence: 99%

Exocytosis of polymorphonuclear leukocyte lysosomal contents induced by dental plaque

White¹,

Montgomery²

1977

Infect Immun

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Rabbit polymorphonuclear leukocytes were incubated with a sonically treated suspension of pooled dental plaque to determine if the plaque would induce release of lysosomal enzymes from the polymorphonuclear leukocytes. Cells incubated with plaque at 37°C released significantly greater amounts of the lysosomal enzymes, 8-glucuronidase and lysozyme, than did cells incubated with plaque at 00C or without plaque at 37°C. This response was both dose and time dependent. Release of the cytoplasmic enzyme lactate dehydrogenase was minimal, and there were no significant differences in lactate dehydrogenase release between cells at 0 and 37°C, or without plaque. These results indicate that dental plaque can induce the selective release of lysosomal enzymes, which could be involved in the periodontal injury produced by dental plaque.

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“…Neutrophils play an essential role in the pathogenesis of inflammation (1). Lysosomal enzymes released from neutrophils stimulated at sites of inflammation are capable of degrading tissue (2).…”

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confidence: 99%

Effect of oxygen tension on human peripheral blood leukocytes: lysosomal enzyme release and metabolic responses during phagocytosis

Jl¹,

Dc²,

Nusinow³

et al. 1981

The Journal of Cell Biology

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We found that nonlethal lysosomal enzyme release from human peripheral blood leukocytes during phagocytosis of opsonized zymosan in vitro was modified by the oxygen tension under which the cells were incubated ; with decreasing PoZ, zymosan-induced release of lysosomal enzymes was potentiated. The effect on enzyme release could not be attributed secondarily to an effect on phagocytosis, because, as others have reported, PoZ had little effect on that response . Metabolic responses that accompany phagocytosis were also modified by oxygen tension . Stimulation of oxidation by way of the pentose cycle was further enhanced by increasing PoZ. Conversely, anaerobic glycolysis was promoted by decreasing oxygen tension . ATP levels fell as a function of time and concentration of phagocytic stimulus, mirroring lysosomal enzyme release as modified by PoZ . Cyclic AMP levels fell during phagocytosis and lysosomal enzyme release, a change that could act to facilitate lysosomal enzyme release. However, the fall in nucleotide level was greatest with highest PoZ (i .e ., when lysosomal enzyme release was least) . The inverse relationship between oxidative metabolism and enzyme release suggested that a product of oxidative metabolism might adversely influence enzyme release. Sulfhydryl antioxidants (cysteine, glutathione) and scavengers of oxygen-derived reactants (superoxide dismutase, catalase, benzoate, hypoxanthine, xanthine, histidine, azide) all potentiated zymosan-stimulated enzyme release. These findings are consistent with the interpretation that one or more factors (e .g ., superoxide anion, hydrogen peroxide, hydroxyl radical, singlet oxygen), generated in association with the burst of oxidative metabolism which accompanies phagocytosis, acts to inhibit lysosomal enzyme release.Neutrophils play an essential role in the pathogenesis of inflammation (1). Lysosomal enzymes released from neutrophils stimulated at sites of inflammation are capable of degrading tissue (2). Toxic by-products of oxidative metabolism may also damage cells and extracellular tissue components (3). Phagocytosis and release of lysosomal enzymes by neutrophils are associated with enhanced glucose oxidation by way of the pentose cycle and enhanced lactate production that results from anaerobic glycolysis (4). Results of experiments in which anaerobic glycolysis was suppressed by metabolic inhibitors have been interpreted as indicating that this pathway must be intact to support both phagocytosis (4) and release of lysosomal enzymes (5). To elucidate further the interrelationships between neutrophil function and metabolism, we have altered cell metabolism by incubating cells in media with varying oxygen tension (PoZ),' a technique that does not depend on the use of drugs or metabolic inhibitors . With this method, we 'Abbreviations used in this paper: H202, hydrogen peroxide; KRP, Krebs-Ringer phosphate buffer; LDH, lactate dehydrogenase; Oz , superoxide anion; OH ., hydroxyl radical; '02, singlet oxygen; Poz, partial pressure of oxygen ; SO...

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Lysosomal Hydrolases and Inflammatory Materials

Cited by 13 publications

References 160 publications

Neutrophil Degranulation

Neutrophil Degranulation

Exocytosis of polymorphonuclear leukocyte lysosomal contents induced by dental plaque

Effect of oxygen tension on human peripheral blood leukocytes: lysosomal enzyme release and metabolic responses during phagocytosis

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