Lysozyme Activity in Cerebrospinal Fluid

Hansen, Niels Ebbe; Karle, Hans; Jensen, Anette; Rock, Elisabeth

doi:10.1111/j.1600-0404.1977.tb05660.x

Cited by 29 publications

(6 citation statements)

References 9 publications

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“… 20 Correlations between soluble MAC components and BBB dysfunction is known from patients with traumatic brain injury, 45 and the positive correlation between complement C9 and age has previously been observed in AD 46 . Earlier studies have reported increased levels of CSF lysozyme as part of an inflammatory reaction to a variety of CNS pathologies 47 . Passage of lysozyme from blood through an impaired BBB as well as local production by microglia within the CNS, have been suggested as the underlying mechanisms.…”

Section: Discussionmentioning

confidence: 94%

Endo‐lysosomal protein concentrations in CSF from patients with frontotemporal dementia caused by CHMP2B mutation

Toft

Sjödin

Simonsen

et al. 2023

Alz & Dem Diag Ass & Dis Mo

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Introduction: Increasing evidence implicates proteostatic dysfunction as an early event in the development of frontotemporal dementia (FTD). This study aimed to explore potential cerebrospinal fluid (CSF) biomarkers associated with the proteolytic systems in genetic FTD caused by CHMP2B mutation. Methods: Combining solid-phase extraction and parallel reaction monitoring mass spectrometry, a panel of 47 peptides derived from 20 proteins was analyzed in CSF from 31 members of the Danish CHMP2B-FTD family.Results: Compared with family controls, mutation carriers had significantly higher levels of complement C9, lysozyme and transcobalamin II, and lower levels of ubiquitin, cathepsin B, and amyloid precursor protein.Discussion: Lower CSF ubiquitin concentrations in CHMP2B mutation carriers indicate that ubiquitin levels relate to the specific disease pathology, rather than all-cause neurodegeneration. Increased lysozyme and complement proteins may indicate innate immune activation. Altered levels of amyloid precursor protein and cathepsins have previously been associated with impaired lysosomal proteolysis in FTD.

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Section: Discussionmentioning

confidence: 94%

Endo‐lysosomal protein concentrations in CSF from patients with frontotemporal dementia caused by CHMP2B mutation

Toft

Sjödin

Simonsen

et al. 2023

Alz & Dem Diag Ass & Dis Mo

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“…As stressed by others (Delpech & Lichtblau 1972, Ganrot & Laurel1 1974, Tourtelotte 1970) the ratios S-AlbumidSp-Albumin and Sp-IgG X S-AlbumidS-IgG x Sp-Albumin have the advantage over the determination of total protein, albumin or IgG in cerebrospinal fluid that variance of the serum concentrations of IgG and albumin is corrected for; hence, lquantitative information is obtained on the size of the barrier for the passage of albumin from blood to cerebrospinal fluid and on the synthesis of IgG in the central nervous system. Though informative, the use of these ratios in diagnostic delineation is hampered by the fact that values outside the reference intervals occur in a variety of neurological diseases (Hansen et al 1977, Olsson & Petterson 1976.…”

Section: Discussionmentioning

confidence: 99%

“…Though informative, the use of these ratios in diagnostic delineation is hampered by the fact that values outside the reference intervals occur in a variety of neurological diseases (Hansen et al 1977, Olsson & Petterson 1976.…”

Section: Patient Data On S-alburnin/sp-albumin and Sp-lgc )< S-4ibummentioning

confidence: 99%

IgG subclasses, barrier function for albumin and production of immunoglobulin G in the central nervous system

Al-Kassab

Dittmann

Olesen

2009

Acta Neurologica Scandinavica

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Cerebrospinal fluids from patients with neurological diseases have been studied. It appeared that the detectability of immunoglobulin G subclasses 1, 2, 3, 4, and the occurrence of oligoclonal bands in the IgG region increased with increasing concentration of IgG in the spinal fluid. No specific pattern emerged in the different disease groups. The reference intervals were determined for: Sp-Albumin(66,OOO): 0.08-4.4 pmol/l; Sp-IgG(160,OOO): 0.06-0.26 pmoV1; S-Albumin : 120413, Sp-AlbuminSp-IgG X S-Albumin within the reference interval, while S-IgG Sp-Albumin was increased. Sp: spinal fluid S-AlbuminSp-IgG X S-Albumin Sp-Albumin S-IgG X Sp-Albumin In these ratios the variability of the concentration of albumin and IgG in serum is taken into account and corrected for.Reference intervals and the values obtained in a variety of neurological diseases for these parameters are presented. and MATERIAL Reference interval samplesCerebrospinal fluid specimens with Sp-Protein (ref. int. 0.15-0.50 g/l), Sp-Erythrocytes and Sp-Leucocytes (ref. int. 0-3 X 106/1) within the reference interval were used to determine the reference intervals for Sp-Albumin(66,000) Sp-Immunoglobulin G S-Albumin Sp-IgG X S-Albumin (160,000), Sp-Albumin and S-IgG Sp-Albumin according to Delpech & Lichtblau (1972) Spinal fluid controlsA pool of 60 reference interval samples of spinal fluid was prepared. The Sp-IgG was 0.15 pmol/l and the Sp-Albumin was 3 pmolll. Part of the pool was concentrated 10 times by dialysis against Carbowax 20 M, and nonconcentrated as well as 10 times concentrated pool was used as control. The pools were stored at -20°C in portions of 1 ml.

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“…-der Qualität und Richtigkeit in Abhängigkeit von den zugrunde liegenden Substraten und Meßver-fahren -sowie in den Angaben der katalytischen Aktivität in Einheiten oder Gewicht pro Völumeneinheit zu unterschiedlichen Ergebnissen geführt (13,7,9 …”

Section: Methodenunclassified

Diagnostische Wertigkeit der Lysozymaktivität bei neurologischen Erkrankungen in Liquor und Serum

Kauerz¹,

Weisner²

1983

CCLM

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Zusammenfassung: Die Lysozymaktivität wurde an Hand der Lyse von Bakterienzellwänden bei Micrococcus lysodeicticus mit der Lysoplate-Methode und mit der Turbidimetrie bestimmt. Beide Methoden wurden miteinander verglichen.Die Lysozymaktivität in Liquor und Serum wurde bei Referenzpersonen (n = 57) und bei Patienten mit bakteriellen Meningoencephalitiden (n = 20), akuten viralen Meningoencephalitiden (n = 2.9), multipler Sklerose (n = 19), chronischen Entzündungen (n = 29), Subarachnoidalblutungen (n = 4), Diskusprotrusionen (n = 12), Polyneuropathien (n = 8), Neoplasmen (n = 28), cerebralen Gefäßprozessen (n = 2), Schädel-Hirn-Traumen (n = 3), metabolischen Myelopathien (n = 7), Hydrocephali (n = 2) bestimmt.

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Lysozyme Activity in Cerebrospinal Fluid

Cited by 29 publications

References 9 publications

Endo‐lysosomal protein concentrations in CSF from patients with frontotemporal dementia caused by CHMP2B mutation

Endo‐lysosomal protein concentrations in CSF from patients with frontotemporal dementia caused by CHMP2B mutation

IgG subclasses, barrier function for albumin and production of immunoglobulin G in the central nervous system

Diagnostische Wertigkeit der Lysozymaktivität bei neurologischen Erkrankungen in Liquor und Serum

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