Lytic enzymes as selectivity means for label-free, microfluidic and impedimetric detection of whole-cell bacteria using ALD-Al2O3 passivated microelectrodes

Couniot, Numa; Vanzieleghem, Thomas; Rasson, Jonathan; Overstraeten-Schlögel, Nancy Van; Poncelet, Olivier; Mahillon, Jacques; Francis, Laurent; Flandre, Denis

doi:10.1016/j.bios.2014.07.084

Cited by 31 publications

(49 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These micrometer dimensions enhance the sensitivity, thanks to the larger active area for bacteria binding and the more confined electric field around adherent bacteria [3,15]. Sinusoidal voltage amplitudes applied on these microelectrodes are typically smaller than the thermal voltage ( 26 mV) to ensure the system linearity [23], which enables the extraction of the complex impedance by a phasorial analysis.…”

Section: Analytical Modelmentioning

confidence: 99%

“…To provide high sensitivity to bacteria, the transducer typically consists of IDEs with gap and width of similar size to bacteria [3]. These micrometer dimensions enhance the sensitivity, thanks to the larger active area for bacteria binding and the more confined electric field around adherent bacteria [3,15].…”

Section: Analytical Modelmentioning

confidence: 99%

“…2a). It is therefore recommended to describe the sensor response in terms of the normalized admittance modulus Y/ω instead of the capacitance [3]. The sensitivity to adherent bacterial cells is then defined as the relative variation of Y/ω , in percent:…”

Section: Analytical Estimation Of the Sensitivitymentioning

confidence: 99%

“…The DL is then screened by the insulator layer for electrolyte ionic strengths C ions > 0.1 mM, since D decreases at higher ionic strength making C DL /C ins larger than 12. The medium conductance G sol R −1 sol and capacitance C sol can be expressed as the sum of the Bacterial impedance ∼d bact , dcyt, t pl , t wall , r,cyt , r,pl , r,wall , cyt , wall -a Al/Al2O3 microelectrodes parameters [3]. b PBS 1:1000 buffer parameters from datasheet and measurements.…”

Section: Electrolyte Without Bacterial Cellsmentioning

confidence: 99%

“…Its variation is related to the amount of biological species that specifically bind on bioreceptors grafted to the sensor surface. Using micrometer-sized interdigitated electrodes (IDEs) also called interdigitated array microelectrodes (IDAM), capacitive biosensors have demonstrated large sensitivity to bacterial cells [2,3] and capability to be easily co-integrated with a CMOS readout interface [4][5][6][7][8], providing miniaturization, system integration, affordability and portability, which are key features for point-ofcare (PoC) diagnosis tools [9].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Capacitive biosensing of bacterial cells: Analytical model and numerical simulations

Couniot

Afzalian

Overstraeten

et al. 2015

Sensors and Actuators B: Chemical

Self Cite

View full text Add to dashboard Cite

Section: Analytical Modelmentioning

confidence: 99%

Section: Analytical Modelmentioning

confidence: 99%

Section: Analytical Estimation Of the Sensitivitymentioning

confidence: 99%

Section: Electrolyte Without Bacterial Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Capacitive biosensing of bacterial cells: Analytical model and numerical simulations

Couniot

Afzalian

Overstraeten

et al. 2015

Sensors and Actuators B: Chemical

Self Cite

View full text Add to dashboard Cite

Weaving Off‐The‐Shelf Yarns into Textile Micro Total Analysis Systems (μTAS)

Månsson

Piper

Hamedi

2020

Macromolecular Bioscience

View full text Add to dashboard Cite

Textile based biosensors have garnered much interest in recent years. Devices woven out of yarns have the ability to be incorporated into clothing and bandages. Most woven devices reported in the literature require yarns that are not available on an industrial scale or that require modifications which are not possible in large scale manufacturing. In this work, commercially produced yarns are taken without any modification or cleaning, and developed woven textile diagnostic devices out of them. The yarn properties that are important to their function within the device have been characterised and discussed. The wicking ability and analyte retention of Coolmax yarns, developed to wick sweat in mass produced sportswear, are determined. The electrochemistry and functionalizability of Au coated multifilament yarns are investigated with no cleaning or treatment and are found to have as good a thiolate self‐assembled monolayer (SAM) coverage as cleaned Au disk electrodes. The feasibility of using these yarns is established off the shelf, with no cleaning, to make woven capillary force driven microfluidic devices and three electrode sensing devices. A proof of principle three electrode system capable of detecting clinically relevant concentrations of glucose in human sweat is reported.

show abstract

Towards a biosensor to monitor the sterilisation efficiency of aseptic filling machines

Oberländer

Bromm

Wendeler

et al. 2015

Physica Status Solidi (a)

View full text Add to dashboard Cite

Sterilisation processes are compulsory in medicine, pharmacy, and food industries to prevent infections of consumers and microbiological contaminations of products. Monitoring the sterilisation by conventional microbiological methods is time‐ and lab‐consuming. To overcome this problem, in this work a novel biosensor has been proposed. The sensor enables a fast method to evaluate sterilisation processes. By means of thin‐film technology the sensor's transducer structures in form of IDEs (interdigitated electrodes) have been fabricated on a silicon substrate. Physical characterisation of the developed sensor was done by AFM, SEM, and profilometry. Impedance analyses were conducted for the electrical characterisation. As microbiological layer spores of B. atrophaeus have been immobilised on the sensing structure; spores of this type are a well‐known sterilisation test organism. Impedance measurements at a fixed frequency over time were performed to monitor the immobilisation process. A sterilisation process according to aseptic filling machines was applied to demonstrate the sensor functionality. After both, immobilisation and sterilisation, a change in impedance could successfully be detected.

show abstract

Lytic enzymes as selectivity means for label-free, microfluidic and impedimetric detection of whole-cell bacteria using ALD-Al2O3 passivated microelectrodes

Cited by 31 publications

References 18 publications

Capacitive biosensing of bacterial cells: Analytical model and numerical simulations

Capacitive biosensing of bacterial cells: Analytical model and numerical simulations

Weaving Off‐The‐Shelf Yarns into Textile Micro Total Analysis Systems (μTAS)

Towards a biosensor to monitor the sterilisation efficiency of aseptic filling machines

Contact Info

Product

Resources

About