M3 muscarinic receptor- and Ca2+ influx-mediated muscle contractions induced by croton oil in isolated rabbit jejunum

Hu, Jing; Gao, Wenyuan; Gao, Ying; Ling, Ning-Sheng; Huang, Lu-Qi; Liu, Changxiao

doi:10.1016/j.jep.2010.04.020

Cited by 29 publications

(32 citation statements)

References 20 publications

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“…A number of earlier investigators have shown that the antioxidant effect of plant products is mainly because of radicals scavenging activity of phenolic compounds such as flavonoids, polyphenols, tannins and phenolic terpenes (Hu et al 2010). These phytochemicals were found to be essential components of V. amygdalina (Adetutu, Oyewo & Adesokan 2013).…”

Section: Discussionmentioning

confidence: 99%

Antidiabetic and anti-oxidant activities of the methanol leaf extract of <i>Vernonia amygdalina</i> in alloxan-induced diabetes in Wistar rats

Adeoye

Oyagbemi

Adedapo

et al. 2017

JOMPED

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The methanolic leaf extract of Vernonia amygdalina (MLVA) was assessed to evaluate its antidiabetic potential in rats. Diabetes was induced in male Wistar rats by the administration of alloxan monohydrate at 100 mg/kg of body weight. After 48 h, rats with fasting blood glucose levels of 200 mg/dL and above were considered diabetic and used for the study. The experimental animals were grouped into five groups (A–E) of 10 animals each. Group A rats were non-diabetic normal control, Group B consisted of diabetic control rats that received no treatment, groups C, D and E rats were diabetic rats but treated with glibenclamide, 200 and 400 mg/kg doses of MLVA respectively. Blood samples were collected at days 14 and 28 after induction for haematological and serum biochemical indices such as triglycerides, LDL, cholesterols etc. The intestine was collected and intestinal homogenate was prepared for the antioxidant studies. The extract at 200 mg/kg and 400 mg/kg doses significantly (p < 0.05) reduced blood glucose levels in extract-treated diabetic rats and also significantly increased weight gain in these rats. Most haematological parameters in treated rats experienced, while platelets and neutrophils were decreased. Biochemical indices measured were reduced in MLVA-treated groups compared with diabetic control. Treatment with MLVA also produced significant (p < 0.05) decrease in markers of oxidative stress but increased levels of enzymic and non-enzymic antioxidant markers in intestinal homogenates of treated groups compared with diabetic control. This study showed that V. amygdalina has antihyperglycaemic and in vivo antioxidant effects.

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Section: Discussionmentioning

confidence: 99%

Antidiabetic and anti-oxidant activities of the methanol leaf extract of <i>Vernonia amygdalina</i> in alloxan-induced diabetes in Wistar rats

Adeoye

Oyagbemi

Adedapo

et al. 2017

JOMPED

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“…In several in vitro studies, the importance of the muscarinic receptor systems on the mechanism of action of plant extracts with the influence of Ca 2+ were discussed in details. For instance, Hu et al [21] studied M3 muscarinic receptor-and Ca 2+ influxmediated muscle contractions induced by croton oil in isolated rabbit jejenum. In the study of Elorriaga et al [22] muscarinic receptor-induced phasic contractions in the rat ileum were depended on the release of internal Ca 2+ entry from the extracellular space through voltage-dependent Ca 2+ channels.…”

Section: Discussionmentioning

confidence: 99%

Contractile Effects of Eryngium kotschyi Boiss. on Rat Isolated Ileum and Detrussor Muscle

Baydan¹,

Kartal²,

Yurdakok³

et al. 2014

Kafkas Univ Vet Fak Derg

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SummaryThe pharmacological activity of the aerial (EKA) and root (EKR) parts of the endemic plant, Eryngium kotschyi Boiss., on rat isolated ileum and detrusor muscle was investigated. Plant extracts alone and with the presence of agonist (acethylcholine) and antagonist (atropin, verapamile, oxybutinine-detrusor muscle, and papaverine-ileum) drugs, along with Ca 2+ applications on calcium-free medium, were applied. Plant extracts induced contraction in ileum and detrusor muscle where the contractions were concentration dependant for EKA and EKR single dose applications in detrusor muscle and concentration-free contractions were observed in cumulative applications for both tissues. Aerial and root parts of Eryngium extracts induced contractions in dose, tissue and protocol dependent manner where the contractions were affected by the tested antagonists, which could be attributed to non-specific pathways including calcium ions and calcium channel stimulations. Keywords: Eryngium kotschyi, Detrusor muscle, Bladder, Ileum, Motility, Rat Eryngium kotschyi Boiss.'in İzole Rat İleum ve İdrar Kesesi Düz Kasında Kastırıcı Etkisi ÖzetBu çalışmada, ülkemizdeki endemik bitkilerden Eryngium kotschyi Boiss. 'in toprak altı (EKTA) ve toprak üstü (EKTU) kısımlarının izole sıçan ileum ve idrar kesesi kasında farmakolojik etkinliği araştırıldı. Bitki ekstrelerinin dokulardaki etkinliği tek, agonist (asetilkolin) ve antagonist (atropin, verapamil, oksibutinin-idrar kesesi, papaverin-ileum) varlığında ve kalsiyumsuz ortamda Ca 2+ uygulamaları ile birlikte değerlendirildi. Bitkinin her iki kısmı doku türü, ekstre dozu ve uygulama protokolüne bağlı değişiklik gösterecek şekilde kontraksiyon oluştururken; bu kasılmaların EKTU ve EKTA tek uygulamalarında doza bağımlı, kümülatif uygulamalarında ise dozdan bağımsız olduğu görüldü. Oluşan kasılmaların test edilen antagonistler ile değiştiği; dolayısıyla kontraktil etkinliğin kalsiyum iyonu ve kalsiyum kanallarının uyarılması gibi nonspesifik yolaklara özellikle bağlı olabileceği görüşüne varıldı.

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“…Relative changes of drug-induced contractile responses to the basal levels (before treatment of drugs) were calculated as percentage. In addition, a standard spasmodic agent Ach (1 M) was used as a positive control and compared with the effect of MeOH extract and the fractions (Hu et al, 2010).…”

Section: Measurement Of Smooth Muscle Contractilitymentioning

confidence: 99%

“…Additionally, it has been reported that low concentrations of croton oil enhance the contractions of jejunal smooth muscle, while high concentrations reduce them (Hu et al, 2010). Moreover, low doses of croton oil increase the GI transit of charcoal and barium meal as well as the production of fecal pellets in mice, while high doses reduced them .…”

Section: Effect Of Meoh Extract and The Fractions On Smooth Muscle Comentioning

confidence: 99%

“…Croton oil, the fatty oil of Crotonis Fructus, has been reported to have a direct effect on guinea pig colonic smooth muscle cell, regulate the GI transit in mice, possesses both spasmogenic and spasmolytic properties, and affect inflammatory and immunological milieu (Wang et al, 2002Hu et al, 2010). Moreover, the aqueous decoction of Crotonis Fructus has also been reported to induce spasmogenic activities in both detrusor smooth muscle of guinea-pig bladder and sphincter of Oddi in rabbits, which may be mediated by intestinal proteins (Qiu et al, 1999;Zhu et al, 1999;Wang et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

M3 muscarinic receptor- and Ca2+ influx-mediated muscle contractions induced by croton oil in isolated rabbit jejunum

Cited by 29 publications

References 20 publications

Antidiabetic and anti-oxidant activities of the methanol leaf extract of <i>Vernonia amygdalina</i> in alloxan-induced diabetes in Wistar rats

Antidiabetic and anti-oxidant activities of the methanol leaf extract of <i>Vernonia amygdalina</i> in alloxan-induced diabetes in Wistar rats

Contractile Effects of Eryngium kotschyi Boiss. on Rat Isolated Ileum and Detrussor Muscle

Activation of M3 muscarinic receptor and Ca2+ influx by crude fraction from Crotonis Fructus in isolated rabbit jejunum

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