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Abstract

Herbal medicines are gaining importance due to more advantages and less toxic effects. Withania coagulans is natural plant that possesses multiple activities. Its main constituents are withaferin and withanolide. The purpose of present study is to identify main constituent of Withania coagulans and preparation of extract loaded micro emulsions. Withania coagulans fruit extract in methanol/chloroform (1:1) was collected in semisolid form and LCMS was done to identify active compound, and then micro emulsions were prepared using Tween 80: Transcutol (1:1) Frankincense oil, and water to enhance its stability for topical application. Five formulations were prepared by Pseudo ternary phase diagram and evaluated for pH, conductivity, viscosity, drug contents, particle size analysis, and polydispersity. Withania coagulans extract was evaluated for anti-bacterial activity against (Staphylococcus aureus, E. coli, and S. typhi) and antifungal activity against (Candida albicans and Aspergillus niger). Anti-inflammatory activity was checked for both extract and Extract based micro emulsion. Among all five formulations F5 shows best physiochemical properties with small globule size, good stability and high anti-inflammatory activity. Based on these results it was concluded that Withania coagulans extract loaded micro emulsions can be used for topical application with promising anti-inflammatory activities. Data for in-vivo studies for checking the topical effect of Withania coagulans is provided elsewhere.

Crest to Trough Cellular Drifting of Green-Synthesized Zinc Oxide and Silver Nanoparticles

“…It is known for its ethnopharmacological application, with diverse biological activities, such as antioxidant, antidiabetic, anti-inflammatory, antihyperglycemic, 27 , 28 hemopoietin, antitumor, anticancer, antimicrobial, immunosuppressive, hepatoprotective, and antidepressant activities. 29 In addition, W. coagulans serves as a reducing agent for nanoparticle synthesis. The well-characterized flower-shaped ZnONPs and spherical AgNPs with a size of 35 and 25 nm, respectively, were used against Staphylococcus aureus and Pseudomonas aeruginosa for in vitro analysis at different concentrations, exhibiting 90.9 and 100% inhibition with ZnONPs and AgNPs with respect to the control.…”

“…This plant is a member of the family Solanaceae and is a traditional plant found in the east of the Mediterranean region expanding to South Asia, commonly being used for the synthesis of NPs. It is known for its ethnopharmacological application, with diverse biological activities, such as antioxidant, antidiabetic, anti-inflammatory, antihyperglycemic, , hemopoietin, antitumor, anticancer, antimicrobial, immunosuppressive, hepatoprotective, and antidepressant activities . In addition, W.…”