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Koike, Toshio; Sakurai, Yoshihisa; Sakawa, Mitsuhiro; Matsuo, Teruo; Shimomura, Yasuto

doi:10.4144/rpsj1954.30.182

Cited by 1 publication

(1 citation statement)

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“…OGDH is a part of the OGDH complex, the mammalian OGDH enzyme complex that catalyzes oxidative decarboxylation of 2-oxoglutarate to succinyl-CoA and CO 2 [62][63][64]. The MDH is a 2nd candidate enzyme of the Krebs cycle that…”

Section: Tca-cycle and A-keto Acid Dehydrogenases Related Proteinsmentioning

confidence: 99%

Potential biomarkers for ischemic heart damage identified in mitochondrial proteins by comparative proteomics

et al. 2006

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We used proteomics to detect regional differences in protein expression levels from mitochondrial fractions of control, ischemia-reperfusion (IR), and ischemic preconditioned (IPC) rabbit hearts. Using 2-DE, we identified 25 mitochondrial proteins that were differentially expressed in the IR heart compared with the control and IPC hearts. For three of the spots, the expression patterns were confirmed by Western blotting analysis. These proteins included 3-hydroxybutyrate dehydrogenase, prohibitin, 2-oxoglutarate dehydrogenase, adenosine triphosphate synthases, the reduced form of nicotinamide adenine dinucleotide (NADH) oxidoreductase, translation elongation factor, actin alpha, malate dehydrogenase, NADH dehydrogenase, pyruvate dehydrogenase and the voltage-dependent anion channel. Interestingly, most of these proteins are associated with the mitochondrial respiratory chain and energy metabolism. The successful use of multiple techniques, including 2-DE, MALDI-TOF-MS and Western blotting analysis demonstrates that proteomic analysis provides appropriate means for identifying cardiac markers for detection of ischemia-induced cardiac injury.

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Section: Tca-cycle and A-keto Acid Dehydrogenases Related Proteinsmentioning

confidence: 99%