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Akbulut, Bilal; Çakmak, Eyüp; Özel, Osman Tolga; Dulger, Nafiye

doi:10.4194/1303-2712-v12_3_15

Cited by 10 publications

(1 citation statement)

References 40 publications

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“…On the other hand, for Siberian sturgeon juveniles, substantially lower concentrations of clove oil (60 to 90 mg/L) were proposed as an appropriate dose range to minimize stress responses [38]. Another previous study on Siberian sturgeon fingerlings has indicated that the effective concentration of clove oil should be 330–381 mg/L [39]. However, in contrast to previous studies, our findings suggest that clove oil is not a suitable anesthetic for Siberian sturgeon prolarvae, due to prolonged recovery times (>8 min to >26 min with test doses 50 to 400 mg/L).…”

Section: Discussionmentioning

confidence: 99%

Anesthetic protocol for microinjection-related handling of Siberian sturgeon (Acipenser baerii; Acipenseriformes) prolarvae

Kim

Nam

2018

PLoS ONE

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An anesthetic protocol was optimized for microinjection-related handling of Siberian sturgeon (Acipenser baerii; Acipenseriformes) prolarvae, an extant primitive fish species commonly grown in aquaculture. Comparative examinations of three selected anesthetics (clove oil, lidocaine, and MS-222) with a dosage regime of 50, 100, 200, and 400 mg/L indicated that MS-222 was the most efficient agent for Siberian sturgeon prolarvae, as evidenced by the fast induction of anesthesia with quick and uniform recovery. Meanwhile, clove oil should be avoided, due to prolonged recovery times varying widely between individuals. None of the tested anesthetics significantly affected prolarval viability at any of the dosage regimes tested in this study. Based on an analysis of the duration of an unconscious state in air, we recommend a dose of 200 mg/L MS-222 for microinjection. Recovery time after use of this dose was influenced by the prolarval age and the development of gills, in which prolarvae older than 3 days after hatching required longer recovery times than did younger prolarvae. Post-recovery behavioral assessment showed no apparent difference between MS-222-anesthetized and non-anesthetized prolarvae in their swimming behavior and phototactic responses. Applicability of currently developed anesthetic protocol using MS-222 in larval microinjection was demonstrated with the injection of a visible dye to the anesthetized prolarvae, followed by the analysis of post-recovery viability. Taken together, the present anesthetic protocol based on 200 mg/L of MS-222 could provide researchers with practical usefulness with good safety margins for the micromanipulation and other related handlings of Siberian sturgeon prolarvae.

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Section: Discussionmentioning

confidence: 99%