Macroencapsulation of human cartilage implants: pilot study with polyelectrolyte complex membrane encapsulation

Haisch, Andreas; Gröger, Andreas; Radke, Cornelia; Ebmeyer, Jörg; Sudhoff, Holger; Grasnick, Gerd; Jähnke, V.; Burmester, Gerd R.; Sittinger, Michael

doi:10.1016/s0142-9612(00)00038-7

Cited by 41 publications

(18 citation statements)

References 30 publications

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“…Tissue engineering of cartilage has enabled the formation of artificial cartilage tissue replacements combining cells, artificial matrices and bioresorbable polymers, in recent years, previous studies have demonstrated promising results for the in vivo and in vitro maturation of tissue-engineered grafts in a variety of cartilage structures, including articular, auricular and nasal septal cartilage transplants (WAKITANI et al, 1989;RODRIGUEZ et al, 1999;HAISCH et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Human platelet supernatant promotes proliferation but not differentiation of articular chondrocytes

Kaps

Loch

Haisch

et al. 2002

Med. Biol. Eng. Comput.

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The objective of the study was to evaluate the growth-promoting activity of human platelet supernatant on primary chondrocytes in comparison with fetal calf serum (FCS) supplemented cell culture medium. Furthermore, the differentiation potential of platelet supernatant was determined in three-dimensional artificial cartilage tissues of bovine articular chondrocytes. Proliferation of articular and nasal septal chondrocytes was assayed by incorporation of BrdU upon stimulation with ten different batches of human platelet supernatant. On bovine articular chondrocytes, all these batches were at least as growth-promoting as FCS. On nasal septal chondrocytes, nine out of ten batches revealed increased or equivalent mitogenic stimulation compared with medium supplemented with FCS. Three-dimensional culture and subsequent histological analysis of matrix formation were used to determine the differentiation properties of platelet supernatant on articular chondrocytes. Human platelet supernatant failed to induce the deposition of typical cartilage matrix components, whereas differentiation and matrix formation were apparent upon cultivation of articular chondrocytes with FCS. Proliferation assays demonstrated that human platelet supernatant stimulates growth of articular and nasal septal chondrocytes; however, platelet supernatant failed to stimulate articular chondrocytes to redifferentiate in three-dimensional chondrocyte cultures. Therefore platelet lysate may be suitable for chondrocyte expansion, but not for maturation of tissue-engineered cartilage.

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Section: Discussionmentioning

confidence: 99%

Human platelet supernatant promotes proliferation but not differentiation of articular chondrocytes

Kaps

Loch

Haisch

et al. 2002

Med. Biol. Eng. Comput.

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“…Chondrogenesis has been extensively studied in murine models by subcutaneous, 13,14 intramuscular, 15 and intraarticular implantations [16][17][18][19][20] of various biomaterials and cells. Rodent joints, however, are small with thin cartilage (Fig.…”

Section: Rodentsmentioning

confidence: 99%

Animal Models for Cartilage Regeneration and Repair

Chu

Szczodry

Bruno

2010

Tissue Engineering Part B: Reviews

460

451

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cartilage injury and degeneration are leading causes of disability. Animal studies are critically important to developing effective treatments for cartilage injuries. This review focuses on the use of animal models for the study of the repair and regeneration of focal cartilage defects. Animals commonly used in cartilage repair studies include murine, lapine, canine, caprine, porcine, and equine models. There are advantages and disadvantages to each model. Small animal rodent and lapine models are cost effective, easy to house, and useful for pilot and proof-of-concept studies. The availability of transgenic and knockout mice provide opportunities for mechanistic in vivo study. Athymic mice and rats are additionally useful for evaluating the cartilage repair potential of human cells and tissues. Their small joint size, thin cartilage, and greater potential for intrinsic healing than humans, however, limit the translational value of small animal models. Large animal models with thicker articular cartilage permit study of both partial thickness and full thickness chondral repair, as well as osteochondral repair. Joint size and cartilage thickness for canine, caprine, and mini-pig models remain significantly smaller than that of humans. The repair and regeneration of chondral and osteochondral defects of size and volume comparable to that of clinically significant human lesions can be reliably studied primarily in equine models. While larger animals may more closely approximate the human clinical situation, they carry greater logistical, financial, and ethical considerations. A multifactorial analysis of each animal model should be carried out when planning in vivo studies. Ultimately, the scientific goals of the study will be critical in determining the appropriate animal model.

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“…The concept of macroencapsulation by polyelectrolyte complex coacervation has been traditionally developed to provide immunoisolation for tissue-engineered constructs [25,26]. Here, we apply this concept to achieve a finer degree of control over the immobilization of cells in 3D scaffolds.…”

Section: Article In Pressmentioning

confidence: 99%

Application of a polyelectrolyte complex coacervation method to improve seeding efficiency of bone marrow stromal cells in a 3D culture system

Toh

Zhou

et al. 2005

Biomaterials

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Macroencapsulation of human cartilage implants: pilot study with polyelectrolyte complex membrane encapsulation

Cited by 41 publications

References 30 publications

Human platelet supernatant promotes proliferation but not differentiation of articular chondrocytes

Human platelet supernatant promotes proliferation but not differentiation of articular chondrocytes

Animal Models for Cartilage Regeneration and Repair

Application of a polyelectrolyte complex coacervation method to improve seeding efficiency of bone marrow stromal cells in a 3D culture system

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