Application of a polyelectrolyte complex coacervation method to improve seeding efficiency of bone marrow stromal cells in a 3D culture system

Toh, Yi‐Chin; Ho, Saey Tuan Barnabas; Zhou, Yi; Hutmacher, Dietmar W.; Yu, Hanry

doi:10.1016/j.biomaterials.2004.10.033

Cited by 41 publications

(35 citation statements)

References 32 publications

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“…Currently, the Andrade and Hlady model is one of the most accepted models for protein adsorption 15 and describes an initially weak interaction, followed by protein denaturation that promotes stronger adhesion. The mechanisms governing the protein-polymer interactions could be polyelectrolyte absorption, 16,17 hydrophobic 18 and ionic interactions, 19 depending on the substrate and type of proteins involved. Importantly, it depicts multilayer adsorption with the outer layers weakly and reversibly adsorbed.…”

Section: Introductionmentioning

confidence: 99%

Protein adsorption onto polyester surfaces: Is there a need for surface activation?

Atthoff¹,

Hilborn²

2006

J Biomed Mater Res

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Surface hydrolysis of polyester scaffolds is a convenient technique suggested to promote protein adsorption for improving cell attachment. We have, therefore, investigated the effect of hydrolysis of polyester surfaces for protein adsorption to clarify the conditions needed. Three polyesters, poly(ethylene terephthalate) (PET), poly(lactic acid) (PLA), and poly(glycolic acid) (PGA), were selected. Adsorption was investigated by X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and quartz crystal microbalance (QCM). Hydrolyzed PET adsorbed significantly more proteins than nonhydrolyzed. Degradable polymers adsorbed at higher rates when the polymers were hydrolyzed prior to adsorption, but the same amount as nonhydrolyzed, suggesting spontaneous hydrolysis during the adsorption. XPS shows that hydrolysis prior to absorption for PET results in a surface nitrogen composition of ϳ14%, similar to pure protein (16%). Nonhydrolyzed PET surfaces showed only ϳ7% nitrogen, indicating protein layers thinner than ϳ10 nm. Adsorption to PLA and PGA shows nitrogen contents of 14 -15% in both cases. SEM revealed striking differences in morphology of the protein coating. Hydrolyzed or spontaneously hydrolyzable surfaces display a pronounced fibrous structure while nonhydrolyzed surfaces give smooth structures. In combination, the results show that surface hydrolysis increase adsorption rate, but not the amount of proteins on polyesters that degrades in vivo. Surface treatment of nondegradable polyester increases the total amount of proteins and induces the formation of fibrous protein structures. Post hydrolysis treatment by acetic acid, replacing the counter-ion to a proton, further enhances protein attachment. Finally, cell attachment experiments verifies that protein adsorption increase the cell attachment to polyester surfaces.

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Section: Introductionmentioning

confidence: 99%

Protein adsorption onto polyester surfaces: Is there a need for surface activation?

Atthoff¹,

Hilborn²

2006

J Biomed Mater Res

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“…The porous tantalum implant was embedded in the hyaluronic acid and the surface area of the implant was increased, so the implant was finally embedded in the membranes after the electrostatic assembly among hyaluronic acid, methylated collagen, and terpolymer of HEMA-MMA-MAA. This was different from the study carried out by Toh et al, 23 which used methylated collagen and terpolymer to macroencapsulate a whole implant.…”

Section: Characterization Of Surface Modification By Semmentioning

confidence: 78%

Electrostatic self-assembly of multilayer copolymeric membranes on the surface of porous tantalum implants for sustained release of doxorubicin

Chen¹

2011

IJN

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Many studies in recent years have focused on surface engineering of implant materials in order to improve their biocompatibility and other performance. Porous tantalum implants have increasingly been used in implant surgeries, due to their biocompatibility, physical stability, and good mechanical strength. In this study we functionalized the porous tantalum implant for sustained drug delivery capability via electrostatic self-assembly of polyelectrolytes of hyaluronic acid, methylated collagen, and terpolymer on the surface of a porous tantalum implant. The anticancer drug doxorubicin was encapsulated into the multilayer copolymer membranes on the porous tantalum implants. Results showed the sustained released of doxorubicin from the functionalized porous tantalum implants for up to 1 month. The drug release solutions in 1 month all had inhibitory effects on the proliferation of chondrosarcoma cell line SW1353. These results suggest that this functionalized implant could be used in reconstructive surgery for the treatment of bone tumor as a local, sustained drug delivery system.

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“…Many polymer-coated particles have been used in the biological field, e.g., microencapsulated systems [85][86][87], polyelectrolyte complex coacervates [88][89][90], or polymer excipient-drug particles. A full account of the applications of nanoparticles for in vitro applications, either for diagnosis (e.g., labelling for MRI or scanners) or therapy (drug delivery, targeted thermotherapy), would in itself require a full review.…”

Section: Biological Applicationsmentioning

confidence: 99%

Polymeric coatings on micro- and nanometric particles for bioapplications

2011

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Particles and notably magnetic particles are used more and more extensively in bioanalytical processes today owing to their ability to considerably facilitate the development of protocols (e.g., easy rinsing and exchange of buffers) and allow miniaturization. Such protocols largely combine the advantages of heterogeneous and homogeneous assays. Applications of these particles, e.g., in protein or nucleic acids purification, immunoassays, or enzymatic microreactors, are developing rapidly, as reflected by the number of commercial kits that are regularly launched. The particles are also increasingly used in microfluidics, in which their potential for miniaturization is at its best. For optimal applications, however, and notably for the analysis and biomolecules and biological samples that are often complex, mastering the interactions between various moleculesparticularly biopolymers such as nucleic acids and proteins and the particle surface-and the grafting of active compounds onto the particles is critical and is indeed the major bottleneck to success. These surface interaction properties are responsible for the direct performance of the particles, such as load capacity and kinetics. Most often, however, the bottlenecks to development and key features for performance lie in mastering the surface properties of the grafted active compounds. These properties are responsible for the intrinsic performance, such as load capacity or reaction kinetics, and are also crucial for the control of spurious effects such as nonspecific adsorption or loss of colloidal stability. By providing an intermediate layer between the particle and its environment, that can be tailored both physically and chemically, polymer coatings offer a very powerful tool for controlling the surface properties of micro-and nanoparticles and for their biofunctionalization. Intense research is being performed in this area, stimulated by the strong potential applications mentioned above, and by the recent development of numerous new chemical routes that simplify the synthesis/ grafting of polymers, such as controlled radical polymerization methods or click chemistry. We provide here a general review of the field. We first give a general overview of the main features and properties of micro-and nanoparticles that are important for bioapplications, such as specific surface area, surface charge, zeta potential, and colloidal stability. We then summarize the different particle morphologies and the configurations that polymers can adopt on their surfaces, from a structural and physical point of view. We also describe the different strategies that can be used to cover particles with polymers, including physisorption, covalent grafting, or direct polymerization onto the particle. This is followed by a section dedicated to the different characterization methods that can be used to master the development of coating protocols, and to control the final properties. We end with a description of a few typical applications, in which the consequences and importance of th...

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Application of a polyelectrolyte complex coacervation method to improve seeding efficiency of bone marrow stromal cells in a 3D culture system

Cited by 41 publications

References 32 publications

Protein adsorption onto polyester surfaces: Is there a need for surface activation?

Protein adsorption onto polyester surfaces: Is there a need for surface activation?

Electrostatic self-assembly of multilayer copolymeric membranes on the surface of porous tantalum implants for sustained release of doxorubicin

Polymeric coatings on micro- and nanometric particles for bioapplications

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